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Rabbit Recombinant Monoclonal Decorin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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Images

Flow Cytometry - Anti-Decorin antibody [EPR24097-105] (AB277636), expandable thumbnail
  • Western blot - Anti-Decorin antibody [EPR24097-105] (AB277636), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (AB277636), expandable thumbnail
  • Western blot - Anti-Decorin antibody [EPR24097-105] (AB277636), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (AB277636), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Expected
Tested
Not recommended
Rat
Not recommended
Not recommended
Expected
Tested
Not recommended

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

May affect the rate of fibrils formation.

Alternative names

SLRR1B, DCN, Decorin, Bone proteoglycan II, PG-S2, PG40

Recommended products

Rabbit Recombinant Monoclonal Decorin antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24097-105
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Flow Cytometry - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Flow Cytometry - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MG-63 (human osteosarcoma fibroblast) cells labelling Decorin with ab277636 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Lower expression tissue: liver (PMID:1611907).

    Exposure time: Lane 1: 8 seconds; Lanes 2-4: 48 seconds.

    All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1: Human uterus tissue lysate at 20 µg

    Lane 2: Human pancreas tissue lysate at 20 µg

    Lane 3: Human placenta tissue lysate at 20 µg

    Lane 4: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 40 kDa

    Observed band size: 90-140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human colon (PMID:32824864). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

    Lane 2: Mouse uterus tissue lysate at 20 µg

    Lane 3: Rat uterus tissue lysate at 20 µg

    Lane 4: Mouse colon tissue lysate at 20 µg

    Lane 5: Rat colon tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 40 kDa

    Observed band size: 90-140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human esophagus (PMID: 19212830). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Negative control: HT-1080 (PMID:8702652).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1: Human pancreas tissue lysate at 20 µg

    Lane 2: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 40 kDa

    Observed band size: 90-140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human bladder cancer. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).

    Exposure time: 15 seconds.

    All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (ab277636) at 1/1000 dilution

    Lane 1: Human colon tissue treated with PNGase F lysate at 20 µg

    Lane 2: Human colon tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 40 kDa

    Observed band size: 90-140 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] (ab277636)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human liver. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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