Rabbit Recombinant Monoclonal Decorin antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--flow-cytometry-img27546.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)")
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img23819.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123768.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)")
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img12110.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127277.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Expected | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Expected | Tested | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
Alternative names
SLRR1B, DCN, Decorin, Bone proteoglycan II, PG-S2, PG40
Recommended products
Rabbit Recombinant Monoclonal Decorin antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24097-105
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab277643 is the carrier-free version of Anti-Decorin antibody [EPR24097-105] ab277636.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--flow-cytometry-img27546.jpg "Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MG-63 (human osteosarcoma fibroblast) cells labelling Decorin with Anti-Decorin antibody [EPR24097-105] ab277636 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img23819.jpg "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).
Lower expression tissue: liver (PMID:1611907).
Exposure time: Lane 1: 8 seconds; Lanes 2-4: 48 seconds.
All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (Anti-Decorin antibody [EPR24097-105] ab277636) at 1/1000 dilution
Lane 1: Human uterus tissue lysate at 20 µg
Lane 2: Human pancreas tissue lysate at 20 µg
Lane 3: Human placenta tissue lysate at 20 µg
Lane 4: Human liver tissue lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 90-140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img123768.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Decorin with Anti-Decorin antibody [EPR24097-105] ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human colon (PMID:32824864). The section was incubated with Anti-Decorin antibody [EPR24097-105] ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img12110.jpg "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).
Exposure time: 48 seconds
All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (Anti-Decorin antibody [EPR24097-105] ab277636) at 1/1000 dilution
Lane 1: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg
Lane 2: Mouse uterus tissue lysate at 20 µg
Lane 3: Rat uterus tissue lysate at 20 µg
Lane 4: Mouse colon tissue lysate at 20 µg
Lane 5: Rat colon tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 40 kDa
Observed band size: 90-140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127277.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling Decorin with Anti-Decorin antibody [EPR24097-105] ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human esophagus (PMID: 19212830). The section was incubated with Anti-Decorin antibody [EPR24097-105] ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img37007.jpg "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).
Negative control: HT-1080 (PMID:8702652).
Exposure time: 3 minutes
All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (Anti-Decorin antibody [EPR24097-105] ab277636) at 1/1000 dilution
Lane 1: Human pancreas tissue lysate at 20 µg
Lane 2: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 40 kDa
Observed band size: 90-140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130220.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Decorin with Anti-Decorin antibody [EPR24097-105] ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human bladder cancer. The section was incubated with Anti-Decorin antibody [EPR24097-105] ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img11172.jpg "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The observed MW is consistent with what has been described in the literature (PMID:12601001, 29561836, 8702652, 12623288).
Exposure time: 15 seconds.
All lanes: Western blot - Anti-Decorin antibody [EPR24097-105] (Anti-Decorin antibody [EPR24097-105] ab277636) at 1/1000 dilution
Lane 1: Human colon tissue treated with PNGase F lysate at 20 µg
Lane 2: Human colon tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 90-140 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82624.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)
This data was developed using Anti-Decorin antibody [EPR24097-105] ab277636, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Decorin with Anti-Decorin antibody [EPR24097-105] ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on stromal cells of human liver. The section was incubated with Anti-Decorin antibody [EPR24097-105] ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img37007.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130220.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
![Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--western-blot-img11172.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643), expandable thumbnail](https://content.abcam.com/products/images/decorin-antibody-epr24097-105-bsa-and-azide-free-ab277643--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82624.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (ab277643)")
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