Anti-Decorin antibody [EPR24097-105] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human liver. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized MG-63 (human osteosarcoma fibroblast) cells labelling Decorin with ab277636 at 1/50 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human bladder cancer. The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human esophagus tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human esophagus (PMID : 19212830). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Decorin with ab277636 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on stromal cells of human colon (PMID : 32824864). The section was incubated with ab277636 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Lab

Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID : 12601001, 29561836, 8702652, 12623288).

Exposure time : 48 seconds

All lanes:

Western blot - Anti-Decorin antibody [EPR24097-105] (<a href='/en-us/products/primary-antibodies/decorin-antibody-epr24097-105-ab277636'>ab277636</a>) at 1/1000 dilution

Lane 1:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Lane 2:

Mouse uterus tissue lysate at 20 µg

Lane 3:

Rat uterus tissue lysate at 20 µg

Lane 4:

Mouse colon tissue lysate at 20 µg

Lane 5:

Rat colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 40 kDa

Observed band size: 90-140 kDa

false

• WB

Lab

Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW is consistent with what has been described in the literature (PMID : 12601001, 29561836, 8702652, 12623288).

Lower expression tissue : liver (PMID : 1611907).

Exposure time : Lane 1 : 8 seconds; Lanes 2-4 : 48 seconds.

All lanes:

Western blot - Anti-Decorin antibody [EPR24097-105] (<a href='/en-us/products/primary-antibodies/decorin-antibody-epr24097-105-ab277636'>ab277636</a>) at 1/1000 dilution

Lane 1:

Human uterus tissue lysate at 20 µg

Lane 2:

Human pancreas tissue lysate at 20 µg

Lane 3:

Human placenta tissue lysate at 20 µg

Lane 4:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 90-140 kDa

false

• WB

Lab

Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID : 12601001, 29561836, 8702652, 12623288).

Negative control : HT-1080 (PMID : 8702652).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Decorin antibody [EPR24097-105] (<a href='/en-us/products/primary-antibodies/decorin-antibody-epr24097-105-ab277636'>ab277636</a>) at 1/1000 dilution

Lane 1:

Human pancreas tissue lysate at 20 µg

Lane 2:

HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 90-140 kDa

false

• WB

Lab

Western blot - Anti-Decorin antibody [EPR24097-105] - BSA and Azide free (AB277643)

This data was developed using ab277636, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The observed MW is consistent with what has been described in the literature (PMID : 12601001, 29561836, 8702652, 12623288).

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-Decorin antibody [EPR24097-105] (<a href='/en-us/products/primary-antibodies/decorin-antibody-epr24097-105-ab277636'>ab277636</a>) at 1/1000 dilution

Lane 1:

Human colon tissue treated with PNGase F lysate at 20 µg

Lane 2:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 40 kDa

Observed band size: 90-140 kDa

false