AB300497

Anti-Dectin-1 antibody [EPR25360-19]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
What is this?

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(3 Publications)

Anti-Dectin-1 antibody [EPR25360-19] is a Rabbit Monoclonal antibody that is used in Dectin-1 IHC-P. Suitable for Mouse samples.

Dectin-1, a C-type lectin receptor, plays a dual role by modulating both pro-tumor and anti-tumor immune responses. It enhances immune responses against tumors like melanoma and breast cancer by activating innate and adaptive immunity. However, in cancers such as pancreatic ductal adenocarcinoma and colorectal cancer, Dectin-1 activation can suppress T cell immunity, facilitating tumor progression. This dual functionality makes Dectin-1 a complex but promising target for cancer immunotherapy.

View Alternative Names

CD369, Bgr, Clecsf12, Dectin1, Clec7a, C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemical analysis of paraffin-embedded Mouse lung cancer tissue labeling Dectin-1 with ab300497 at 1/500 (1.06 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse lung cancer. The section was incubated with ab300497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Dectin-1 with ab300497 at 1/500 (1.06 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control : No staining on mouse cerebrum. The section was incubated with ab300497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Dectin-1 with ab300497 at 1/500 (1.06 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse spleen (PMID : 15107454). The section was incubated with ab300497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Dectin-1 with ab300497 at 1/500 (1.06 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse lung. The section was incubated with ab300497 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Carrier free

Anti-Dectin-1 antibody [EPR25360-19] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25360-19

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed : 11544516, PubMed : 15213161, PubMed : 17159984). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B : upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed : 12719479, PubMed : 15731053, PubMed : 16825490, PubMed : 32358020). Enhances cytokine production in macrophages and dendritic cells (PubMed : 15845454). Mediates production of reactive oxygen species in the cell (PubMed : 12719479, PubMed : 15731053, PubMed : 16825490). Mediates phagocytosis of C.albicans conidia (PubMed : 15729357, PubMed : 16825490). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation (PubMed : 10779524). Stimulates T-cell proliferation (PubMed : 10779524). Induces phosphorylation of SCIMP after binding beta-glucans (PubMed : 27288407).

See full target information Clec7a

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The journal of headache and pain 26:158 PubMed40640733

2025

Spi1 aggravates neuropathic pain by modulating Clec7a-mediated neuroinflammation and microglial phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Xu,Xinli Liu,Hui Chen,Yun Zhao,Yubai Zhao,Yu Xie,Jiahui Pang,Hui Zeng,Yanyan Zeng,Weiwei Peng,Manxu Zheng,Wen Wu

Advanced materials (Deerfield Beach, Fla.) 36:e2304333 PubMed38096399

2023

Inflammation-Targeting Mesenchymal Stem Cells Combined with Photothermal Treatment Attenuate Severe Joint Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jun Shin,Jun-Young Park,Jun Young Park,Su Hyun Lim,Hyoungsub Lim,Jin Kyeong Choi,Chul-Kyu Park,Youn Joo Kang,Dongwoo Khang

Science advances 9:eade0293 PubMed37235655

2023

Secreted endogenous macrosomes reduce Aβ burden and ameliorate Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Cunli Wang,Yiming Yang,Xiaoyu Zhang,Zhenqiang Shi,Huiling Gao,Manli Zhong,Yonggang Fan,Hongyan Zhang,Bo Liu,Guangyan Qing

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dectin-1 antibody [EPR25360-19]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dectin-1 antibody [EPR25360-19] (AB300497)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise