Rabbit Recombinant Monoclonal Dectin-1 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Dectin-1 antibody [EPR28032-27] (AB311845), expandable thumbnail](https://content.abcam.com/products/images/dectin-1-antibody-epr28032-27-ab311845--immunocytochemistry-immunofluorescence-img416952.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dectin-1 antibody [EPR28032-27] (AB311845)")
![Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (AB311845), expandable thumbnail](https://content.abcam.com/products/images/dectin-1-antibody-epr28032-27-ab311845--flow-cytometry-img416986.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (AB311845)")
![Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (AB311845), expandable thumbnail](https://content.abcam.com/products/images/dectin-1-antibody-epr28032-27-ab311845--flow-cytometry-img416985.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (AB311845)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed:11567029, PubMed:12423684). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (By similarity). Enhances cytokine production in macrophages and dendritic cells (By similarity). Mediates production of reactive oxygen species in the cell (By similarity). Mediates phagocytosis of C.albicans conidia (PubMed:17230442). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation. Stimulates T-cell proliferation. Induces phosphorylation of SCIMP after binding beta-glucans (By similarity).
Alternative names
CD369, BGR, CLECSF12, DECTIN1, UNQ539/PRO1082, CLEC7A, C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1
Recommended products
Rabbit Recombinant Monoclonal Dectin-1 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28032-27
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Dectin-1 antibody [EPR28032-27] (ab311845), expandable thumbnail](https://content.abcam.com/products/images/dectin-1-antibody-epr28032-27-ab311845--immunocytochemistry-immunofluorescence-img416952.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dectin-1 antibody [EPR28032-27] (ab311845)")
Immunocytochemistry/ Immunofluorescence - Anti-Dectin-1 antibody [EPR28032-27] (ab311845)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7(human breast adenocarcinoma epithelial cell) cells labelling Dectin-1 with ab311845 at 1/500 (1.008 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in MCF7 cell line, no staining was observed in Jurkat cell line.Negative control: Jurkat (PMID:33803858).Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (ab311845), expandable thumbnail](https://content.abcam.com/products/images/dectin-1-antibody-epr28032-27-ab311845--flow-cytometry-img416986.jpg "Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (ab311845)")
Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (ab311845)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Dectin-1 with ab311845 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cell co-stained with CD3 conjugated to A647. Gated on viable cells.
![Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (ab311845), expandable thumbnail](https://content.abcam.com/products/images/dectin-1-antibody-epr28032-27-ab311845--flow-cytometry-img416985.jpg "Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (ab311845)")
Flow Cytometry - Anti-Dectin-1 antibody [EPR28032-27] (ab311845)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling Dectin-1 with ab311845 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cell co-stained with CD14 conjugated to A647. Gated on viable cells.
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