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AB324238

Anti-Dectin-1 antibody [EPR29712-507]

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Rabbit Recombinant Monoclonal Dectin-1 antibody. Suitable for WB and reacts with Mouse samples.

View Alternative Names

CD369, Bgr, Clecsf12, Dectin1, Clec7a, C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1

2 Images
Western blot - Anti-Dectin-1 antibody [EPR29712-507] (AB324238)
  • WB

Supplier Data

Western blot - Anti-Dectin-1 antibody [EPR29712-507] (AB324238)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Neuro-2a (PMID : 10779524)

Low expression : liver, skin (PMID : 10779524)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10779524).

The identity of the band at approximately 15 kDa in lane 1 is likely to be N-Term target fragments..

The identity of the band at approximately 100 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 59 seconds; Lanes 3-5 : 114 seconds

All lanes:

Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238) at 1/1000 dilution

Lane 1:

N9 (mouse microglia cell) whole cell lysate at 40 µg

Lane 2:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

Lane 3:

Mouse spleen tissue lysate at 40 µg

Lane 4:

Mouse liver tissue lysate at 40 µg

Lane 5:

Mouse skin tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25-40 kDa,36 kDa

false

Western blot - Anti-Dectin-1 antibody [EPR29712-507] (AB324238)
  • WB

Supplier Data

Western blot - Anti-Dectin-1 antibody [EPR29712-507] (AB324238)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : Neuro-2a (PMID : 10779524)

Dectin-1 is a glycoprotein of approximately 25-40 kDa and detected as 20-35 kDa band after treated with Deglycosylation Peptide : N-glycosidase F (PNGase F).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238) at 1/1000 dilution

Lane 1:

Untreated N9 (mouse microglia cell) whole cell lysate at 20 µg

Lane 2:

N9 whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Lane 3:

Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

Neuro-2a whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 25-40 kDa,36 kDa

false

Exposure time: 125s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29712-507

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB309390

Human Dectin-1 ELISA Kit

Sandwich ELISA - Human Dectin-1 ELISA Kit (AB309390)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed : 11544516, PubMed : 15213161, PubMed : 17159984). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B : upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed : 12719479, PubMed : 15731053, PubMed : 16825490, PubMed : 32358020). Enhances cytokine production in macrophages and dendritic cells (PubMed : 15845454). Mediates production of reactive oxygen species in the cell (PubMed : 12719479, PubMed : 15731053, PubMed : 16825490). Mediates phagocytosis of C.albicans conidia (PubMed : 15729357, PubMed : 16825490). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation (PubMed : 10779524). Stimulates T-cell proliferation (PubMed : 10779524). Induces phosphorylation of SCIMP after binding beta-glucans (PubMed : 27288407).
See full target information Clec7a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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