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Rabbit Recombinant Monoclonal Dectin-1 antibody. Suitable for WB and reacts with Mouse samples.

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Images

Western blot - Anti-Dectin-1 antibody [EPR29712-507] (AB324238), expandable thumbnail
  • Western blot - Anti-Dectin-1 antibody [EPR29712-507] (AB324238), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Target data

Function

Lectin that functions as a pattern recognizing receptor (PRR) specific for beta-1,3-linked and beta-1,6-linked glucans, which constitute cell wall constituents from pathogenic bacteria and fungi (PubMed:11544516, PubMed:15213161, PubMed:17159984). Necessary for the TLR2-mediated inflammatory response and activation of NF-kappa-B: upon beta-glucan binding, recruits SYK via its ITAM motif and promotes a signaling cascade that activates some CARD domain-BCL10-MALT1 (CBM) signalosomes, leading to the activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:12719479, PubMed:15731053, PubMed:16825490, PubMed:32358020). Enhances cytokine production in macrophages and dendritic cells (PubMed:15845454). Mediates production of reactive oxygen species in the cell (PubMed:12719479, PubMed:15731053, PubMed:16825490). Mediates phagocytosis of C.albicans conidia (PubMed:15729357, PubMed:16825490). Binds T-cells in a way that does not involve their surface glycans and plays a role in T-cell activation (PubMed:10779524). Stimulates T-cell proliferation (PubMed:10779524). Induces phosphorylation of SCIMP after binding beta-glucans (PubMed:27288407).

Alternative names

CD369, Bgr, Clecsf12, Dectin1, Clec7a, C-type lectin domain family 7 member A, Beta-glucan receptor, C-type lectin superfamily member 12, Dendritic cell-associated C-type lectin 1, DC-associated C-type lectin 1, Dectin-1

Rabbit Recombinant Monoclonal Dectin-1 antibody. Suitable for WB and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29712-507
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238), expandable thumbnail

    Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238)

    Dectin-1 Western blot staining using rabbit Anti-Dectin-1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Neuro-2a (PMID: 10779524)

    Low expression: liver, skin (PMID: 10779524)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 10779524).

    The identity of the band at approximately 15 kDa in lane 1 is likely to be N-Term target fragments..

    The identity of the band at approximately 100 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 59 seconds; Lanes 3-5: 114 seconds

    All lanes: Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238) at 1/1000 dilution

    Lane 1: N9 (mouse microglia cell) whole cell lysate at 40 µg

    Lane 2: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 40 µg

    Lane 3: Mouse spleen tissue lysate at 40 µg

    Lane 4: Mouse liver tissue lysate at 40 µg

    Lane 5: Mouse skin tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 25-40 kDa, 36 kDa

  • Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238), expandable thumbnail

    Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238)

    Dectin-1 Western blot staining using rabbit Anti-Dectin-1 antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: Neuro-2a (PMID: 10779524)

    Dectin-1 is a glycoprotein of approximately 25-40 kDa and detected as 20-35 kDa band after treated with Deglycosylation Peptide:N-glycosidase F (PNGase F).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Dectin-1 antibody [EPR29712-507] (ab324238) at 1/1000 dilution

    Lane 1: Untreated N9 (mouse microglia cell) whole cell lysate at 20 µg

    Lane 2: N9 whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

    Lane 3: Untreated Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 4: Neuro-2a whole cell lysate treated with Protein Deglycosylation Peptide:N-glycosidase F (PNGase F) at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 25-40 kDa, 36 kDa

    Exposure time: 125s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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