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AB325354

Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free

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Rabbit Recombinant Monoclonal DECTIN 2 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

Clec4n, Clecsf10, Dectin2, Clec6a, C-type lectin domain family 6 member A, C-type lectin superfamily member 10, Dendritic cell-associated C-type lectin 2, DC-associated C-type lectin 2, Dectin-2

3 Images
Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free (AB325354)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free (AB325354)

This data was developed using ab325347, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow cells labelling DECTIN 2 with ab325347 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®488.

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free (AB325354)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free (AB325354)

This data was developed using ab325347, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow cells labelling DECTIN 2 with ab325347 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free (AB325354)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] - BSA and Azide free (AB325354)

This data was developed using ab325347, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse bone marrow derived Dendritic cells induced by 20ng/ml GM-CSF for 13 days and 50ng/ml IL4 for 7 days (Lower left and right) Mouse bone marrow (Upper left and right) cells labelling DECTIN 2 with ab325347 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells. Cells were co-stained with anti-CD11c conjugated to Brilliant Violet 421.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30285-562

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab325354 is the carrier-free version of ab325347

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system : specifically recognizes and binds alpha-mannans on C.albicans hypheas (PubMed : 17050534, PubMed : 19703985, PubMed : 20493731). Binding of C.albicans alpha-mannans to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (PubMed : 17050534, PubMed : 19703985, PubMed : 20493731, PubMed : 32358020). Recognizes also, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production (PubMed : 19124755). Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses (PubMed : 21059925).
See full target information Clec6a
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com