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Rabbit Recombinant Monoclonal DEK antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (AB166624), expandable thumbnail
  • Western blot - Anti-DEK antibody [EPR11034] (AB166624), expandable thumbnail
  • Immunoprecipitation - Anti-DEK antibody [EPR11034] (AB166624), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] (AB166624), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (AB166624), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/250
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Involved in chromatin organization.

Alternative names

Protein DEK, DEK

Recommended products

Rabbit Recombinant Monoclonal DEK antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR11034
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

DEK is a nuclear phosphoprotein with an approximate mass of 45 kDa. Researchers often study DEK in the context of its function in chromatin organization and transcription regulation as it serves as an architectural chromatin protein. DEK expression is ubiquitous found in various tissues with high levels in actively dividing cells. It is essential in processes that require dynamic chromatin remodeling.

Biological function summary

DEK plays a role in maintaining genomic integrity and regulating transcription. It operates within a complex that interacts with histone proteins to modulate chromatin structure. DEK also affects mRNA splicing by interacting with the spliceosomal complex further underlining its role in transcription control. As a multifunctional protein DEK helps coordinate several essential cellular activities.

Pathways

DEK integrates into the p53 and Wnt signaling pathways. It influences cell cycle control and apoptosis within the p53 pathway interacting with proteins like MDM2 to affect p53 stability. Within the Wnt pathway DEK contributes to cell proliferation and differentiation by affecting beta-catenin levels and interacting with transcription factors involved in Wnt target gene expression.

Associated diseases and disorders

DEK has strong connections to various types of cancers and autoimmune diseases. DEK overexpression is often associated with tumorigenesis particularly in acute myeloid leukemia where it collaborates with NUP214 fusion partners. In autoimmune disorders like systemic lupus erythematosus DEK may become an autoantigen and its interaction with proteins such as nucleolin could contribute to disease pathogenesis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

    Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DEK with ab166624 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Western blot - Anti-DEK antibody [EPR11034] (ab166624)

    All lanes: Western blot - Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution

    Lane 1: 293T cell lysates at 10 µg

    Lane 2: HeLa cell lysates at 10 µg

    Lane 3: Jurkat cell lysates at 10 µg

    Lane 4: K562 cell lysates at 10 µg

    Predicted band size: 42 kDa

  • Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624)

    Immunoprecipitation of DEK in pellet from HeLa cell lysate using ab166624 at 1/10 dilution, followed by WB detection of DEK using as secondary an HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

    All lanes: Immunoprecipitation - Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution

    All lanes: immunoprecipitation pellet from HeLa cell lysate at 10 µg

    Predicted band size: 42 kDa

  • Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] (ab166624)

    Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DEK (red) with ab166624 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-DEK antibody [EPR11034] (ab166624)

    Immunofluorescence analysis of 293T cells labeling DEK with ab166624 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEK antibody [EPR11034] (ab166624)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DEK with ab166624 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-DEK antibody [EPR11034] (ab166624), expandable thumbnail

    Western blot - Anti-DEK antibody [EPR11034] (ab166624)

    DEK Western blot staining using rabbit Anti-DEK antibody

    Western blot: Rabbit Monoclonal[EPR11034] to DEK ab166624 staining at 1/2000 dilution, shown in green; Mouse anti-CANX (Anti-Calnexin antibody [CANX/1543] ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 50 kDa in Wild-type HeLa cell lysates with no signal observed at this size in DEK knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

    All lanes: Western blot - Anti-DEK antibody [EPR11034] (ab166624) at 1/2000 dilution

    Lane 1: Wild-type HeLa at 20 µg

    Lane 2: Human DEK knockout HeLa cell line at 20 µg

    Lane 3: T-47D at 20 µg

    Lane 4: K562 at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 43 kDa

    Observed band size: 50 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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