Anti-delta 1 Catenin/CAS antibody [EPR357(2)]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

Paraffin embedded human breast carcinoma tissue (panel 1) and human colonic adenocarcinoma tissue (panel 2) labeled with unpurified ab92514 at 1/100 dilution.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian cancer tissue sections labeling delta 1 Catenin/CAS with purified ab92514 at 1 : 50 dilution (1.84 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

A431 (Human epidermoid carcinoma cell line) cells stained for delta 1 Catenin/CAS using unpurified ab92514 at a dilution of 1/100 in ICC/IF.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling delta 1 Catenin/CAS with unpurified ab92514 at 1/20 dilution (10μg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab92514 at 1 : 50 dilution (2.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Lab

Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

The multiple bands are isoforms caused by alternatively splicing as was described in PMID 9653641 and are consistent with what has been descirbed in PMID 15077190.

All lanes:

Western blot - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (ab92514) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysates at 15 µg

Lane 3:

RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg

Lane 4:

C2C12 (Mouse myoblasts myoblast) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 108 kDa

Observed band size: 70-120 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-delta 1 Catenin/CAS antibody [EPR357(2)] (AB92514)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.