Rabbit Recombinant Monoclonal delta 1 Catenin/CAS antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Human samples.
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
IHC-P | Flow Cyt (Intra) | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Chicken | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Primary antibody incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Chicken, Cow, Pig | Dilution info - | Notes - |
Select an associated product type
Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability (PubMed:14610055, PubMed:20371349). Promotes localization and retention of DSG3 at cell-cell junctions, via its interaction with DSG3 (PubMed:18343367). Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics (PubMed:10207085, PubMed:20371349). Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors (PubMed:17344476).
KIAA0384, CTNND1, Catenin delta-1, Cadherin-associated Src substrate, p120 catenin, p120(cas), CAS, p120(ctn)
Rabbit Recombinant Monoclonal delta 1 Catenin/CAS antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF and reacts with Human samples.
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Purified from TCS by protein A/G.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Delta 1 Catenin also known as CAS or δ-catenin is a member of the p120-catenin subfamily weighing around 122 kDa. It is predominantly expressed in the central nervous system where it localizes at the synapses. Apart from its neural distribution delta 1 Catenin also appears in epithelial tissues. This protein plays an important role in cell-cell adhesion by interacting with cadherins at the cytoplasmic side of the plasma membrane thereby facilitating the formation and maintenance of adherens junctions.
Delta 1 Catenin participates in the regulation of synaptic stability and plasticity. As part of a protein complex it binds to the juxtamembrane domain of cadherins influencing actin cytoskeletal dynamics. Through this interaction delta 1 Catenin modulates signal transduction pathways that are essential for neuronal differentiation and synapse formation. In epithelial cells it is also involved in creating cell shapes and promoting tissue integrity.
Delta 1 Catenin is essential in the Wnt signaling and Rho GTPase pathways. Within these pathways its interaction with E-cadherin and other catenins influences gene expression and cellular behaviors like migration and proliferation. The Wnt signaling pathway includes proteins such as β-catenin which works alongside delta 1 Catenin to regulate transcriptional activities pivotal for developmental processes and cancer progression.
Delta 1 Catenin has associations with neurodegenerative diseases and certain cancers. In Alzheimer’s disease alterations in delta 1 Catenin may disrupt synaptic connectivity contributing to cognitive decline. In cancer particularly prostate and colorectal its interaction with proteins like β-catenin potentially leads to changes in cell adhesion and metastasis. Such involvement makes delta 1 Catenin a significant target for therapeutic intervention.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab227638. Cells were fixed in 100% Methanol. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast ductal cell carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human cervix tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human endometrium adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human esophagus tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for delta 1 Catenin/CAS using ab227638 at 1/100 dilution in immunohistochemical analysis.
Flow cytometry analysis of A431 (human epidermoid carcinoma epithelial cell) labeling delta 1 Catenin/CAS with purified ab227638 at (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlableled control -Unlabelled cells / blue.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com