JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247228

Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal delta 1 Catenin/CAS antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0384, CTNND1, Catenin delta-1, Cadherin-associated Src substrate, p120 catenin, p120(cas), CAS, p120(ctn)

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)

This data was developed using ab32095, the same antibody clone in a different buffer formulation.

ab32095 at a 1/100 dilution staining delta 1 Catenin/CAS in human breast carcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)

This data was developed using ab32095, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling delta 1 Catenin/CAS with purified ab32095 at 1/100 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)
  • IP

Lab

Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)

This data was developed using ab32095, the same antibody clone in a different buffer formulation.
Purified ab32095 at 1/50 dilution (2μg) immunoprecipitating delta 1 Catenin/CAS in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab32095 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32095 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 120 kDa
Multi-bands could be isoforms of delta 1 Catenin based on Uniprot.

All lanes:

Immunoprecipitation - Anti-delta 1 Catenin/CAS antibody [YE372] (<a href='/en-us/products/primary-antibodies/delta-1-catenin-cas-antibody-ye372-ab32095'>ab32095</a>)

Predicted band size: 108 kDa

false

Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)
  • WB

Unknown

Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] - BSA and Azide free (AB247228)

This data was developed using ab32095, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-delta 1 Catenin/CAS antibody [YE372] (<a href='/en-us/products/primary-antibodies/delta-1-catenin-cas-antibody-ye372-ab32095'>ab32095</a>) at 1/5000 dilution

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

Predicted band size: 108 kDa

Observed band size: 120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

YE372

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

ab247228 reacts with an epitope located in the N terminal region of delta 1 Catenin/CAS

Specificity

This antibody is specific for human delta 1 Catenin/CAS (isoform 1ABC and 968AA). It also detects splice isoforms 1, 1A, 1B, 1C, 1AB, 1AC, 1BC, 2, 2A, 2B, 2C, 2AB, 2AC, 2BC and 2ABC of delta 1 Catenin/CAS.

style
left-column

Complementary Products

Primary Antibodies

AB177294

Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)]

RabMAb|Advanced Validation|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (di methyl K56) antibody [EPR17684(2)] (AB177294)

  • 0
  • 0
Primary Antibodies

AB232982

Anti-GTF2H1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GTF2H1 antibody (AB232982)

  • 0
  • 0
Primary Antibodies

AB5131

Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody

ChIP - Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) antibody (AB5131)

  • 4
  • 48
Primary Antibodies

AB63766

Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (AB63766)

  • 5
  • 7
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

ab247228 is the carrier-free version of ab32095.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta 1 Catenin also known as CAS or δ-catenin is a member of the p120-catenin subfamily weighing around 122 kDa. It is predominantly expressed in the central nervous system where it localizes at the synapses. Apart from its neural distribution delta 1 Catenin also appears in epithelial tissues. This protein plays an important role in cell-cell adhesion by interacting with cadherins at the cytoplasmic side of the plasma membrane thereby facilitating the formation and maintenance of adherens junctions.
Biological function summary

Delta 1 Catenin participates in the regulation of synaptic stability and plasticity. As part of a protein complex it binds to the juxtamembrane domain of cadherins influencing actin cytoskeletal dynamics. Through this interaction delta 1 Catenin modulates signal transduction pathways that are essential for neuronal differentiation and synapse formation. In epithelial cells it is also involved in creating cell shapes and promoting tissue integrity.

Pathways

Delta 1 Catenin is essential in the Wnt signaling and Rho GTPase pathways. Within these pathways its interaction with E-cadherin and other catenins influences gene expression and cellular behaviors like migration and proliferation. The Wnt signaling pathway includes proteins such as β-catenin which works alongside delta 1 Catenin to regulate transcriptional activities pivotal for developmental processes and cancer progression.

Delta 1 Catenin has associations with neurodegenerative diseases and certain cancers. In Alzheimer’s disease alterations in delta 1 Catenin may disrupt synaptic connectivity contributing to cognitive decline. In cancer particularly prostate and colorectal its interaction with proteins like β-catenin potentially leads to changes in cell adhesion and metastasis. Such involvement makes delta 1 Catenin a significant target for therapeutic intervention.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability (PubMed : 14610055, PubMed : 20371349). Promotes localization and retention of DSG3 at cell-cell junctions, via its interaction with DSG3 (PubMed : 18343367). Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics (PubMed : 10207085, PubMed : 20371349). Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors (PubMed : 17344476).
See full target information CTNND1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1617737 PubMed40746860

2025

Epiplakin expression is lost in psoriatic skin lesions and is downregulated by IFN-γ in skin cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Hannes Kühtreiber,Corinne Drexler,Melanie Salek,Lisa Auer,Johannes Griss,Michael Mildner,Peter Fuchs
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com