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AB247506

Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal delta 1 Catenin/CAS phospho S268 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0384, CTNND1, Catenin delta-1, Cadherin-associated Src substrate, p120 catenin, p120(cas), CAS, p120(ctn)

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free (AB247506)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free (AB247506)

This data was developed using ab79545, the same antibody clone in a different buffer formulation.

ab79545 at 1/100 dilution staining delta 1 Catenin/CAS in paraffin-embedded human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free (AB247506)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free (AB247506)

This data was developed using ab79545, the same antibody clone in a different buffer formulation.

Immunofluorescent staining of delta 1 Catenin/CAS in A431 cells using ab79545 at 1/100 dilution.

Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free (AB247506)
  • WB

Unknown

Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] - BSA and Azide free (AB247506)

This data was developed using ab79545, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-delta 1 Catenin/CAS (phospho S268) antibody [EPR2380] (<a href='/en-us/products/primary-antibodies/delta-1-catenin-cas-phospho-s268-antibody-epr2380-ab79545'>ab79545</a>) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HeLa cell lysate treated with Calyculin A at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 108 kDa

Observed band size: 90-120 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2380

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab247506 is the carrier-free version of ab79545.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta 1 Catenin also known as CAS or δ-catenin is a member of the p120-catenin subfamily weighing around 122 kDa. It is predominantly expressed in the central nervous system where it localizes at the synapses. Apart from its neural distribution delta 1 Catenin also appears in epithelial tissues. This protein plays an important role in cell-cell adhesion by interacting with cadherins at the cytoplasmic side of the plasma membrane thereby facilitating the formation and maintenance of adherens junctions.
Biological function summary

Delta 1 Catenin participates in the regulation of synaptic stability and plasticity. As part of a protein complex it binds to the juxtamembrane domain of cadherins influencing actin cytoskeletal dynamics. Through this interaction delta 1 Catenin modulates signal transduction pathways that are essential for neuronal differentiation and synapse formation. In epithelial cells it is also involved in creating cell shapes and promoting tissue integrity.

Pathways

Delta 1 Catenin is essential in the Wnt signaling and Rho GTPase pathways. Within these pathways its interaction with E-cadherin and other catenins influences gene expression and cellular behaviors like migration and proliferation. The Wnt signaling pathway includes proteins such as β-catenin which works alongside delta 1 Catenin to regulate transcriptional activities pivotal for developmental processes and cancer progression.

Delta 1 Catenin has associations with neurodegenerative diseases and certain cancers. In Alzheimer’s disease alterations in delta 1 Catenin may disrupt synaptic connectivity contributing to cognitive decline. In cancer particularly prostate and colorectal its interaction with proteins like β-catenin potentially leads to changes in cell adhesion and metastasis. Such involvement makes delta 1 Catenin a significant target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability (PubMed : 14610055, PubMed : 20371349). Promotes localization and retention of DSG3 at cell-cell junctions, via its interaction with DSG3 (PubMed : 18343367). Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics (PubMed : 10207085, PubMed : 20371349). Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors (PubMed : 17344476).
See full target information CTNND1 phospho S268
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:1142-1154 PubMed40229600

2025

Longitudinal single-cell multiomic atlas of high-risk neuroblastoma reveals chemotherapy-induced tumor microenvironment rewiring.

Applications

Unspecified application

Species

Unspecified reactive species

Wenbao Yu,Rumeysa Biyik-Sit,Yasin Uzun,Chia-Hui Chen,Anusha Thadi,Jonathan H Sussman,Minxing Pang,Chi-Yun Wu,Liron D Grossmann,Peng Gao,David W Wu,Aliza Yousey,Mei Zhang,Christina S Turn,Zhan Zhang,Shovik Bandyopadhyay,Jeffrey Huang,Tasleema Patel,Changya Chen,Daniel Martinez,Lea F Surrey,Michael D Hogarty,Kathrin Bernt,Nancy R Zhang,John M Maris,Kai Tan
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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