Rabbit Recombinant Monoclonal delta 1 Catenin/CAS phospho Y228 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ICC/IF | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Tested |
Mouse | Not recommended | Not recommended | Predicted | Predicted |
Rat | Not recommended | Not recommended | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Key regulator of cell-cell adhesion that associates with and regulates the cell adhesion properties of both C-, E- and N-cadherins, being critical for their surface stability (PubMed:14610055, PubMed:20371349). Promotes localization and retention of DSG3 at cell-cell junctions, via its interaction with DSG3 (PubMed:18343367). Beside cell-cell adhesion, regulates gene transcription through several transcription factors including ZBTB33/Kaiso2 and GLIS2, and the activity of Rho family GTPases and downstream cytoskeletal dynamics (PubMed:10207085, PubMed:20371349). Implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors (PubMed:17344476).
KIAA0384, CTNND1, Catenin delta-1, Cadherin-associated Src substrate, p120 catenin, p120(cas), CAS, p120(ctn)
Rabbit Recombinant Monoclonal delta 1 Catenin/CAS phospho Y228 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
E205
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab247254 is the carrier-free version of Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] ab32403.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Delta 1 Catenin also known as CAS or δ-catenin is a member of the p120-catenin subfamily weighing around 122 kDa. It is predominantly expressed in the central nervous system where it localizes at the synapses. Apart from its neural distribution delta 1 Catenin also appears in epithelial tissues. This protein plays an important role in cell-cell adhesion by interacting with cadherins at the cytoplasmic side of the plasma membrane thereby facilitating the formation and maintenance of adherens junctions.
Delta 1 Catenin participates in the regulation of synaptic stability and plasticity. As part of a protein complex it binds to the juxtamembrane domain of cadherins influencing actin cytoskeletal dynamics. Through this interaction delta 1 Catenin modulates signal transduction pathways that are essential for neuronal differentiation and synapse formation. In epithelial cells it is also involved in creating cell shapes and promoting tissue integrity.
Delta 1 Catenin is essential in the Wnt signaling and Rho GTPase pathways. Within these pathways its interaction with E-cadherin and other catenins influences gene expression and cellular behaviors like migration and proliferation. The Wnt signaling pathway includes proteins such as β-catenin which works alongside delta 1 Catenin to regulate transcriptional activities pivotal for developmental processes and cancer progression.
Delta 1 Catenin has associations with neurodegenerative diseases and certain cancers. In Alzheimer’s disease alterations in delta 1 Catenin may disrupt synaptic connectivity contributing to cognitive decline. In cancer particularly prostate and colorectal its interaction with proteins like β-catenin potentially leads to changes in cell adhesion and metastasis. Such involvement makes delta 1 Catenin a significant target for therapeutic intervention.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Terms & Conditions.
This data was developed using Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] ab32403, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] ab32403)
Lane 1: HeLa cell lysate, untreated
Lane 2: HeLa cell lysate, treated with Pervanandate
Lane 3: HeLa cell lysate, treated with Phosphatase
Predicted band size: 108 kDa
Observed band size: 115 kDa
This data was developed using Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] ab32403, the same antibody clone in a different buffer formulation.Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] ab32403, at a 1/50 dilution, staining delta 1 Catenin/CAS in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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