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AB184917

Anti-Delta-catenin antibody [EPR17628]

4

(4 Reviews)

|

(4 Publications)

Rabbit Recombinant Monoclonal Delta-catenin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Catnd2, Nprap, Ctnnd2, Catenin delta-2, Neural plakophilin-related ARM-repeat protein, Neurojungin, NPRAP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (AB184917)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] (AB184917)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling delta-catenin with ab184917 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and nuclear staining on Neuro-2a cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab184917 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

Western blot - Anti-Delta-catenin antibody [EPR17628] (AB184917)
  • WB

Supplier Data

Western blot - Anti-Delta-catenin antibody [EPR17628] (AB184917)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time = 10 seconds

All lanes:

Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 135 kDa

false

Western blot - Anti-Delta-catenin antibody [EPR17628] (AB184917)
  • WB

Supplier Data

Western blot - Anti-Delta-catenin antibody [EPR17628] (AB184917)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time = 3 minutes

All lanes:

Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

Lane 1:

Rat hippocampus lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 135 kDa

false

Western blot - Anti-Delta-catenin antibody [EPR17628] (AB184917)
  • WB

Supplier Data

Western blot - Anti-Delta-catenin antibody [EPR17628] (AB184917)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time = 10 seconds

All lanes:

Western blot - Anti-Delta-catenin antibody [EPR17628] (ab184917) at 1/2000 dilution

Lane 1:

Mouse hippocampus lysate at 10 µg

Lane 2:

Mouse cerebellum lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17628

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Delta-catenin also known as CTNND2 or Neurojungin is an important protein involved in cellular adhesion processes. With a molecular mass of around 122 kDa Delta-catenin is found mainly in the brain and nervous system tissues. It is a member of the armadillo/β-catenin superfamily and plays a significant role in the structure and function of synapses connecting cytoskeletal components to cell junctions. Its expression is most prominent in neurons suggesting a function important to neuronal development and maintenance.
Biological function summary

Delta-catenin associates with and is part of the cadherin-catenin complex facilitating cell-cell adhesion in neuronal cells. This complex supports the stabilization of adherens junctions influencing cell shape signaling and gene transcription. Delta-catenin's interaction with cadherin proteins helps regulate synaptic plasticity and dendritic spine development impacting learning and memory processes within the brain.

Pathways

Delta-catenin contributes significantly to the Wnt signaling pathway. It plays a role in cellular edge interactions and migration by modulating cadherin-mediated adhesion and Rho GTPase signaling. In collaboration with proteins such as β-catenin Delta-catenin influences processes linked to cell morphological changes and neurodevelopmental regulation. It acts as a mediator between intracellular signaling and intercellular communication impacting neurogenesis.

Delta-catenin has potential links to neurodevelopmental disorders like autism spectrum disorder (ASD) and Alzheimer's disease. Alterations in Delta-catenin expression and mutations correlate with cognitive dysfunction and synaptic impairments observed in these conditions. In Alzheimer's disease Delta-catenin's interaction with β-amyloid and presenilin-1 proteins can exacerbate synaptic loss and memory deficits identifying it as a possible target in understanding disease mechanisms and therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has a critical role in neuronal development, particularly in the formation and/or maintenance of dendritic spines and synapses (PubMed : 17993462, PubMed : 25807484). Involved in the regulation of canonical Wnt signaling (By similarity). It probably acts on beta-catenin turnover, facilitating beta-catenin interaction with GSK3B, phosphorylation, ubiquitination and degradation (PubMed : 20623542). May be involved in neuronal cell adhesion and tissue morphogenesis and integrity by regulating adhesion molecules. Functions as a transcriptional activator when bound to ZBTB33 (PubMed : 15282317).
See full target information Ctnnd2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1 PubMed39747445

2025

Fortilin binds CTNNA3 and protects it against phosphorylation, ubiquitination, and proteasomal degradation to guard cells against apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Nakashima,Decha Pinkaew,Uttariya Pal,Fei Miyao,Hanna Huynh,Lena Tanaka,Ken Fujise

The Journal of cell biology 222: PubMed37707499

2023

δ-Catenin controls astrocyte morphogenesis via layer-specific astrocyte-neuron cadherin interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Christabel Xin Tan,Dhanesh Sivadasan Bindu,Evelyn J Hardin,Kristina Sakers,Ryan Baumert,Juan J Ramirez,Justin T Savage,Cagla Eroglu

Frontiers in genetics 13:867872 PubMed36303547

2022

Delta-catenin attenuates medulloblastoma cell invasion by targeting EMT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanjun Hu,Sihan Zhu,Rizhen Xu,Manxia Wang,Furong Chen,Zeshun Zhang,Binghong Feng,Jian Wang,Zhongping Chen,Jing Wang

Biochemical and biophysical research communication 518:239-245 PubMed31421822

2019

Hypoxia-induced lncRNA EIF3J-AS1 accelerates hepatocellular carcinoma progression via targeting miR-122-5p/CTNND2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yang,Bowen Yao,Yongshen Niu,Tianxiang Chen,Huanye Mo,Liang Wang,Cheng Guo,Demao Yao
View all publications
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Product promise

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