Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (AB250781)

This data was developed using ab184917, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling delta-catenin with ab184917 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasmic and nuclear staining on Neuro-2a cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab184917 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

• WB

Supplier Data

Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (AB250781)

This data was developed using ab184917, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Delta-catenin antibody [EPR17628] (<a href='/en-us/products/primary-antibodies/delta-catenin-antibody-epr17628-ab184917'>ab184917</a>) at 1/2000 dilution

Lane 1:

Rat hippocampus lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 135 kDa

false

• WB

Supplier Data

Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (AB250781)

This data was developed using ab184917, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Delta-catenin antibody [EPR17628] (<a href='/en-us/products/primary-antibodies/delta-catenin-antibody-epr17628-ab184917'>ab184917</a>) at 1/2000 dilution

Lane 1:

Mouse hippocampus lysate at 10 µg

Lane 2:

Mouse cerebellum lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 135 kDa

false

• WB

Supplier Data

Western blot - Anti-Delta-catenin antibody [EPR17628] - BSA and Azide free (AB250781)

This data was developed using ab184917, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Delta-catenin antibody [EPR17628] (<a href='/en-us/products/primary-antibodies/delta-catenin-antibody-epr17628-ab184917'>ab184917</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 132 kDa

Observed band size: 135 kDa

false