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AB246339

Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Delta Opioid Receptor antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

OPRD, OPRD1, Delta-type opioid receptor, D-OR-1, DOR-1

4 Images
Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)

Intracellular flow cytometric analysis of U87-MG cells labeling Delta Opioid Receptor using ab176324 at a 1/10 dilution (red)or a rabbit IgGcontrol (green).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324).

Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y cells labelling Delta Opioid Receptor at 1/500. Cells were fixed with 100% Methanol. An ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control : primary antibody (1/500) and secondary antibody, ab150077 AlexaFluor® 488 Goat anti-Rabbit secondary IgG (1/1000).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324).

Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)

Intracellular Flow Cytometry analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/150 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324)

Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Delta Opioid Receptor antibody [EPR5029(2)] - BSA and Azide free (AB246339)

Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Delta Opioid Receptor with purified ab176324 at 1/100 dilution (10 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab176324)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5029(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab246339 is the carrier-free version of ab176324.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Delta Opioid Receptor also known as DOR is a G protein-coupled receptor involved in the modulation of pain mood and neuroprotection. It is a transmembrane protein with a mass around 40 kDa. The receptor is often expressed in the central nervous system particularly in areas such as the brain and spinal cord. Due to its role in analgesic pathways the receptor's function is of great interest for pain management therapies. Present in various tissues the presence of delta opioid receptors can influence different physiological processes.
Biological function summary

Delta opioid receptors participate in the modulation of neurotransmitter release neuronal excitability and neurogenesis. These receptors form part of a complex that includes other opioid receptors such as mu and kappa receptors interacting to fine-tune the body's response to pain and stress. Activation of delta opioid receptors results in analgesic effects making them potential targets for therapeutic interventions in pain management. Their expression in peripheral tissues also suggests roles in immune and inflammatory responses.

Pathways

Delta opioid receptors fit into the opioid receptor signaling cascades that influence pain perception and reward mechanisms. The major pathways include the adenylate cyclase inhibition pathway which reduces the production of cAMP and the MAPK cascade promoting cell survival and differentiation. These receptors commonly interact with G proteins such as Gi/o proteins to mediate cellular responses. Delta 5029 and delta 193 are segments of research focusing on specific pathways where delta 193 relates to receptor trafficking and delta 5029 involves receptor desensitization mechanisms.

Delta opioid receptors are linked to conditions like chronic pain and depression. Alterations in delta opioid receptor expression or function contribute to these disorders suggesting their involvement in pathological states. Through their involvement in opioid signaling they connect to disorders characterized by altered brain chemistry such as depression. Proteins interacting with delta receptors in these contexts include other opioid receptors which share overlapping roles in emotional regulation and pain perception. Antagonists or agonists like anti-delta antibodies are researched for potential therapeutic applications in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor that functions as a receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.
See full target information OPRD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 33:698-712 PubMed37662970

2023

Selection of a novel cell-internalizing RNA aptamer specific for CD22 antigen in B cell acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Dario Ruiz-Ciancio,Li-Hsien Lin,Suresh Veeramani,Maya N Barros,Diego Sanchez,Ary Lautaro Di Bartolo,Diego Masone,Paloma H Giangrande,María Belén Mestre,William H Thiel
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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