Anti-delta Sarcoglycan antibody [EPR8706]

6 product images

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  Immunoprecipitation - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  Purified ab137101 at 1/20 dilution (1μg) immunoprecipitating delta Sarcoglycan in Human skeletal muscle lysate.
  Lane 1 (input): Human skeletal muscle lysate 10μg
  Lane 2 (+): ab137101 + Human skeletal muscle lysate.
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab137101 in Human skeletal muscle lysate.
  VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
  Blocking Buffer and concentration: 5% NFDM/TBST.
  Diluting buffer and concentration: 5% NFDM/TBST.
  Observed band size: 35 kDa

  All lanes: Immunoprecipitation - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  Predicted band size: 32 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  Immunohistochemical analysis of paraffin-embedded Human heart tissue labelling delta Sarcoglycan with ab137101 at 1/100 dilution.
  

  Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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  Western blot - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  All lanes: Western blot - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101) at 1/1000 dilution

  Lane 1: Human fetal heart lysate at 10 µg

  Lane 2: Human skeletal muscle lysate at 10 µg

  Secondary

  All lanes: Goat anti-rabbit HRP at 1/2000 dilution

  Predicted band size: 32 kDa

  Observed band size: 35 kDa

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  Multiplex immunohistochemistry - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  Fluorescence multiplex immunohistochemical analysis of the human cardiac muscle (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-delta Sarcoglycan (ab137101, red; Opal™690), anti-Titin (Anti-Titin antibody [EPR26167-75] ab307446, green; Opal™520) and anti-Natriuretic peptides A (Anti-Natriuretic peptides A antibody [EPR20247] ab209232, magenta; Opal™570) on human cardiac muscle. Panel B: anti-Titin displayed nucleus and cytoplasm expression. Panel C: anti-Natriuretic peptides A displayed granular cytoplasmic expression. Panel D: anti-delta Sarcoglycan displayed membrane expression. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of ab137101 at 1/1000 (1.043 μg/ml) dilution, Anti-Titin antibody [EPR26167-75] ab307446 at 1/500 (0.95 μg/ml) dilution, and Anti-Natriuretic peptides A antibody [EPR20247] ab209232 at 1/3000 (0.241 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
  
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-delta Sarcoglycan (ab137101, green; Opal™690), anti-Fast Myosin Skeletal Heavy chain + MYH4 (Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] ab221149, magenta; Opal™520) and anti-Slow Skeletal Myosin Heavy chain (Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] ab234431, red; Opal™570) on human skeletal muscle. Panel B: anti-Fast Myosin Skeletal Heavy chain + MYH4 stained on fast type fibers. Panel C: anti-Slow Skeletal Myosin Heavy chain stained on slow type fibers. Panel D: anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of ab137101 at 1/1000 (1.043 μg/ml), Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] ab221149 at 1/1000 (0.505 μg/ml, and Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] ab234431 at 1/4000 (0.255 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
  
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Multiplex immunohistochemistry - Anti-delta Sarcoglycan antibody [EPR8706] (ab137101)

  Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections).
  
  Panel A: merged staining of anti-delta Sarcoglycan (ab137101, red; Opal™690), anti-MYH2 (Anti-MYH2 antibody [A4.74] ab264036, cyan; Opal™520) and anti-MYH6 + Slow Skeletal Myosin Heavy chain (Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free ab250868, gray; Opal™570) on human skeletal muscle. Panel B: anti-MYH2 stained on the fast twitch type 2A fibers. Panel C: anti-MYH6 + Slow Skeletal Myosin Heavy chain stained on skeletal muscle fiber subtypes. Panel D: anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of ab137101 at 1/1000 dilution (1.043 μg/ml), Anti-MYH2 antibody [A4.74] ab264036 at 1/1000 dilution (1.116 μg/ml) and Anti-MYH6 + Slow Skeletal Myosin Heavy chain antibody [EPR10891(2)] - BSA and Azide free ab250868 1/5000 dilution (0.209 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.
  
  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
  
  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.