AB73331

Anti-DeltaD antibody [Dld 5C7/2, zdd2]

Name: Anti-DeltaD antibody [Dld 5C7/2, zdd2] (ab73331) | Abcam
Brand: Abcam Limited
SKU: AB73331
Rating: 4 (1 reviews)

(1 Review)

(12 Publications)

Mouse Monoclonal DeltaD antibody. Suitable for Flow Cyt, ELISA, WB, ICC/IF, IHC - Wmt, IHC-Fr and reacts with Zebrafish samples. Cited in 12 publications. Immunogen corresponding to Native Full Length Protein corresponding to Zebrafish dld.

View Alternative Names

aei, dld, Delta-like protein D, DeltaD, After eight protein

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Dld 5C7/2, zdd2

Isotype

IgG1

Carrier free

Reacts with

Zebrafish

Applications

WB, IHC-Fr, Flow Cyt, ICC/IF, ELISA, IHC - Wmt

applications

Immunogen

Native Full Length Protein corresponding to Zebrafish dld.

Q8UWJ4

Epitope

Zebrafish DeltaD protein extracellular domain.

Specificity

This antibody recognises the extracellular domain of zebrafish DeltaD. This antibody does not detect DeltaC; but it cross-reacts weakly with DeltaA.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 99.98% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DeltaD also known as Notch ligand Delta-like 3 is a member of the Delta/Notch family. It has a molecular mass of approximately 60 kDa. Expression of DeltaD occurs mainly in human embryonic tissues though it is also present in some adult tissues including brain and muscle. The Notch signaling pathway where DeltaD functions as a ligand is essential for communication between adjacent cells via binding with Notch receptors.

Biological function summary

Proteins from the Delta/Notch family influence numerous developmental processes. DeltaD participates in the regulation of cell fate determination and differentiation during embryonic development. It works within the Notch signaling pathway where it forms a complex with Notch receptors and other ligands to control the transcription of target genes. The modulation of Notch signaling by DeltaD is important for proper tissue patterning and morphogenesis.

Pathways

Many cellular activities rely on DeltaD's interaction with the Notch signaling pathway. This pathway influences critical processes such as angiogenesis and neurogenesis. Within these framework DeltaD interacts with several Notch receptors such as Notch1 and Notch2 to regulate gene expression. The interplay between DeltaD and these receptors ensures the correct execution of developmental programs and cellular differentiation.

Dysregulation of DeltaD expression associates with congenital conditions such as spondylocostal dysostosis and neural tube defects. Anomalies in the Notch signaling pathway where DeltaD acts can disrupt normal development resulting in such pathologies. Notch1 and other family members are involved in the pathophysiology of these disorders emphasizing DeltaD's role in maintaining proper developmental signaling and tissue function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as a ligand for Notch receptors and is involved in primary neurogenesis and somitogenesis. Can activate Notch receptors, thereby playing a key role in lateral inhibition, a process that prevents the immediate neighbors of each nascent neural cell from simultaneously embarking on neural differentiation. Required in somite segmentation to keep the oscillations of neighboring presomitic mesoderm cells synchronized.

See full target information dld

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 12:e4336 PubMed35592606

2022

Colocalization Analysis for Cryosectioned and Immunostained Tissue Samples with or without Label Retention Expansion Microscopy (LR-ExM) by JACoP.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhao,Jason Garcia,Loic A Royer,Su Guo

The EMBO journal 40:e107134 PubMed34180064

2021

LncRNA VEAL2 regulates PRKCB2 to modulate endothelial permeability in diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Paras Sehgal,Samatha Mathew,Ambily Sivadas,Arjun Ray,Jyoti Tanwar,Sushma Vishwakarma,Gyan Ranjan,K V Shamsudheen,Rahul C Bhoyar,Abhishek Pateria,Elvin Leonard,Mukesh Lalwani,Archana Vats,Rajeev R Pappuru,Mudit Tyagi,Saumya Jakati,Shantanu Sengupta,Binukumar B K,Subhabrata Chakrabarti,Inderjeet Kaur,Rajender K Motiani,Vinod Scaria,Sridhar Sivasubbu

Science advances 7: PubMed34117063

2021

Polarized endosome dynamics engage cytoplasmic Par-3 that recruits dynein during asymmetric cell division.

Applications

WB, ICC

Species

Zebrafish, Zebrafish

Xiang Zhao,Jason Q Garcia,Kai Tong,Xingye Chen,Bin Yang,Qi Li,Zhipeng Dai,Xiaoyu Shi,Ian B Seiple,Bo Huang,Su Guo

Scientific reports 9:9958 PubMed31292468

2019

Notch-mediated inhibition of neurogenesis is required for zebrafish spinal cord morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Sharma,Vishnu Muraleedharan Saraswathy,Li Xiang,Maximilian Fürthauer

Developmental biology 429:225-239 PubMed28648842

2017

Pnrc2 regulates 3'UTR-mediated decay of segmentation clock-associated transcripts during zebrafish segmentation.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas L Gallagher,Kiel T Tietz,Zachary T Morrow,Jasmine M McCammon,Michael L Goldrich,Nicolas L Derr,Sharon L Amacher

Methods in molecular biology (Clifton, N.J.) 1576:273-281 PubMed28434166

2017

Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Tong,Mahendra Wagle,Su Guo

Developmental biology 381:179-88 PubMed23747599

2013

CaMK-II activation is essential for zebrafish inner ear development and acts through Delta-Notch signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah C Rothschild,Jamie Lahvic,Ludmila Francescatto,Jamie J A McLeod,Shawn M Burgess,Robert M Tombes

PloS one 6:e19076 PubMed21533047

2011

The transcriptional mediator component Med12 is required for hindbrain boundary formation.

Applications

IHC - Wmt

Species

Zebrafish

Sung-Kook Hong,Igor B Dawid

Development (Cambridge, England) 138:1705-15 PubMed21429985

2011

Assembly and patterning of the vascular network of the vertebrate hindbrain.

Applications

Unspecified application

Species

Unspecified reactive species

Misato Fujita,Young R Cha,Van N Pham,Atsuko Sakurai,Beth L Roman,J Silvio Gutkind,Brant M Weinstein

Development (Cambridge, England) 132:1093-104 PubMed15689380

2005

Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Cécile Crosnier,Neil Vargesson,Stephen Gschmeissner,Linda Ariza-McNaughton,Alastair Morrison,Julian Lewis

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DeltaD antibody [Dld 5C7/2, zdd2]

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise