JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB308407

Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Mouse Monoclonal Dengue virus 2 Capsid protein antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Dengue virus 2 samples.
4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)

For immunofluorescence analysis, mock and infected A549 cells were fixed in 4% paraformaldehyde at room temperature for 1 hour and permeabilized for detection of Dengue virus Capsid protein using ab308407 at 1/510 dilution (green). Blue : nuclear staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)

Immunohistochemical analysis of paraffin-embedded BHK-21 infected with dengue virus labeling Dengue virus Capsid protein using ab308407 at 1/1000 dilution (green). Blue : Fluoroshield with DAPI. Antigen retrieval was performed using Citrate buffer, pH 6.0, 15 min

Western blot - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)
  • WB

Supplier Data

Western blot - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)

Non-infected (–) and infected (+) C6/36 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Capsid protein antibody [GT574] (ab308407) diluted at 1/1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (ab308407) at 1/1000 dilution

Lane 1:

Non-infected (–) C6/36 whole cell extracts at 15 µg

Lane 2:

Infected (+) C6/36 whole cell extracts at 15 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

false

Western blot - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)
  • WB

Supplier Data

Western blot - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (AB308407)

Non-infected (–) and infected (+) BHK-21 whole cell extracts (15 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Capsid protein antibody [GT574] (ab308407) diluted at 1/1000.

All lanes:

Western blot - Anti-Dengue virus Capsid protein antibody [GT574] - BSA and Azide free (ab308407) at 1/1000 dilution

Lane 1:

Non-infected (–) BHK-21 whole cell extracts at 15 µg

Lane 2:

Infected (+) BHK-21 whole cell extracts at 15 µg

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT574

Isotype

IgG2a

Carrier free

Yes

Reacts with

Dengue virus 2

Applications

WB, ICC/IF, IHC-P

applications

style
left-column

Complementary Products

Proteins & Peptides

AB285976

Recombinant EN-TEV Protease protein

Bioactive

Size Exclusion Chromatography - Recombinant EN-TEV Protease protein (AB285976)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Dengue virus 2": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/510", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dengue Virus 2 often called Dengue Type 2 is a serotype of the dengue virus a significant vector-borne pathogen. The virus has a positive-sense single-stranded RNA genome encoding several structural and non-structural proteins including the capsid protein and NS5. The capsid protein weighing approximately 12 kDa is important for encapsulating the viral RNA forming the nucleocapsid. NS5 with a mass around 103 kDa functions as a methyltransferase and RNA-dependent RNA polymerase essential for the viral replication cycle. These proteins are expressed predominantly in host cells after the virus infects facilitating its life cycle and pathogenesis.
Biological function summary

Within host cells the capsid protein and NS5 are involved in viral assembly and RNA synthesis respectively. The capsid protein enables efficient packaging of the viral genome interacting with several cellular components for the assembly of new virions. NS5 engages in the methylation of viral RNA and replication of the viral genome. NS5 does not act alone; it works as part of a larger replication complex involving other viral proteins such as NS3 which provides helicase activity further supporting the functionality of the viral replication machinery.

Pathways

Dengue Virus 2 proteins play key roles in the viral replication and immune evasion pathways. The replication complex containing NS5 and other non-structural proteins orchestrates the replication pathway ensuring the production of viral progeny. The capsid protein is associated with the budding of new virions within the endoplasmic reticulum which is important for the release of infectious particles. In addition NS5 interacts with host proteins such as STAT2 modulating immune pathways to conceal the virus from host defenses enabling persistent viral replication and infection.

Dengue Virus 2 is related to Dengue fever and severe forms like Dengue hemorrhagic fever. The NS5 protein through its interaction with STAT2 plays a role in immune suppression affecting disease outcomes. The capsid protein's strong involvement in viral assembly links it to the efficient dissemination of the virus contributing to the severity and viral load in dengue infections. Understanding these interactions is essential for future therapeutic strategies aimed at mitigating dengue-related health issues worldwide.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Dengue virus 2 Capsid protein
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com