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AB317175

Anti-Dengue virus NS3 protein antibody [HL2239]

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Rabbit Monoclonal antibody. Suitable for WB and reacts with Transfected cell lysate - Dengue virus 2, Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4 samples.
1 Images
Western blot - Anti-Dengue virus NS3 protein antibody [HL2239] (AB317175)
  • WB

Supplier Data

Western blot - Anti-Dengue virus NS3 protein antibody [HL2239] (AB317175)

Non-infected and infected BHK-21 whole cell extracts (5 ug) were separated by 7.5% SDS-PAGE and the membrane was blotted with ab317175 diluted at 1 : 200000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Dengue virus NS3 protein antibody [HL2239] (ab317175) at 1/200000 dilution

Lane 1:

Mock-infected BHK-21 whole cell extracts at 5 µg

Lane 2:

JEV infected BHK-21 whole cell extracts at 5 µg

Lane 3:

Zika infected BHK-21 whole cell extracts at 5 µg

Lane 4:

Dengue virus 1 infected BHK-21 whole cell extracts at 5 µg

Lane 5:

Dengue virus 2 infected BHK-21 whole cell extracts at 5 µg

Lane 6:

Dengue virus 3 infected BHK-21 whole cell extracts at 5 µg

Lane 7:

Dengue virus 4 infected BHK-21 whole cell extracts at 5 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2239

Isotype

IgG

Carrier free

No

Reacts with

Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4

Applications

WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Dengue virus 1": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000", "WB-species-notes": "<p></p>" }, "Dengue virus 2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000", "WB-species-notes": "<p></p>" }, "Dengue virus 3": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000", "WB-species-notes": "<p></p>" }, "Dengue virus 4": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Dengue virus 2": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dengue Virus NS3 also known as NS3 protein is a non-structural protein with enzyme activity mainly acting as a serine protease and RNA helicase. Weighing approximately 69 kDa NS3 is expressed in infected cells of vertebrate hosts and is an important component in the virus replication process. It requires NS2B as a cofactor to display its protease activity facilitating the cleavage of the viral polyprotein precursor into functional proteins.
Biological function summary

NS3 protein plays a significant role in viral replication and maturation by partaking in the intracellular processes of the dengue virus. NS3 acts in concert with other non-structural proteins to ensure efficient replication of the viral genome. Particularly the helicase function assists in unwinding RNA during genome synthesis making it indispensable for the viral life cycle.

Pathways

NS3 protein participates in the dengue virus replication and maturation pathways which are critical for viral propagation. It interacts directly with other dengue virus proteins like NS2B and NS5 the latter functioning as the viral polymerase. NS3 and NS5 establish a multi-protein replicase complex that facilitates RNA genome replication and processing.

NS3 protein has a direct connection to dengue fever a widespread tropical disease caused by dengue virus infection. Its interaction with host immune proteins leads to pathogenic effects contributing to disease symptoms and progression. The NS3 protein's activity is a potential target for antiviral drug development aiming to inhibit its protease and helicase functions to reduce viral replication and mitigate disease severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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