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AB41473

Anti-Dengue Virus prM glycoprotein antibody [DM1]

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(9 Publications)

Mouse Monoclonal POLG antibody. Suitable for ELISA, WB, ICC/IF and reacts with Dengue virus 2 samples. Cited in 9 publications.

View Alternative Names

pre-membrane glycoprotein

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus prM glycoprotein antibody [DM1] (AB41473)
  • ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Dengue Virus prM glycoprotein antibody [DM1] (AB41473)

Immunofluorescence of Vero cells infected with Dengue Virus 2 using ab41473 (green).

Western blot - Anti-Dengue Virus prM glycoprotein antibody [DM1] (AB41473)
  • WB

Unknown

Western blot - Anti-Dengue Virus prM glycoprotein antibody [DM1] (AB41473)

All lanes:

Western blot - Anti-Dengue Virus prM glycoprotein antibody [DM1] (ab41473)

Lanes 1 - 2:

Dengue Virus 1 infected C6/36 cell lysate

Lanes 3 - 4:

Dengue Virus 2 (NGC) infected C6/36 cell lysate

Lanes 5 - 6:

Dengue Virus 2 (16681) infected C6/36 cell lysate

Lanes 7 - 8:

Dengue Virus 3 infected C6/36 cell lysate

Lanes 9 - 10:

Dengue Virus 4 infected C6/36 cell lysate

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DM1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Dengue virus 2

Applications

ICC/IF, ELISA, WB

applications

Specificity

This antibody reacts with the pre-membrane glycoprotein of Dengue Virus 2 in Western blot. In ELISA, it recognises prM glycoprotein from Dengue Virus 1,2,3 and 4.

Using non-reduced and unheated samples to preserve conformational epitopes, as this antibodies recognize conformational rather than linear epitopes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Dengue virus 2": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/5 - 1/20", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.1% Proclin 150 Constituents: 89.9% RPMI 1640, 10% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Dengue Virus prM glycoprotein also known as pre-membrane protein is a critical component in the lifecycle of the dengue virus. This glycoprotein has a molecular mass of approximately 22 to 23 kDa. It is expressed on the surface of immature virions in host cells where it undergoes conformational changes. During viral maturation prM shields the fusion loop of the envelope (E) protein preventing premature fusion during viral replication and assembly. This action supports the assembly of mature infectious virions by eventually cleaving into the membrane (M) protein under the influence of furin protease.
Biological function summary

The Dengue Virus prM glycoprotein plays an important role in virus maturation and infectivity. It forms a complex with the envelope glycoprotein to protect viral components during transport and to ensure proper assembly. This complex is necessary for the virus to remain intact as it exits the host cell facilitating successful infection of new cells. The cleavage of prM into the M protein by cellular proteases remains an indispensable process that dictates the infectiosity and virulence of the virus.

Pathways

The role of the prM protein is highly significant in the viral entry and maturation pathways. The cleavage of prM glycoprotein occurs in the endoplasmic reticulum and trans-Golgi network involving key cellular processes that modify viral particles. This process is closely associated with the functionality of other viral proteins such as the envelope (E) protein which contributes to the virus’s ability to bind and fuse with host cell membranes. This coordination ensures the efficient production of mature dengue virions ready for replication.

The Dengue Virus prM glycoprotein is a major focus in the study of dengue fever and severe dengue like dengue hemorrhagic fever. The prM protein's involvement in the maturation pathway directly affects virulence and infectivity making it an attractive target for vaccine development and therapeutic interventions. During infection antibodies developed against prM and the related envelope (E) protein can aid in neutralization but they may also contribute to antibody-dependent enhancement (ADE) which can exacerbate the severity of the disease. Understanding prM's role provides insight into potential strategies for preventing and managing dengue-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Dengue Virus prM glycoprotein
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Intervirology 63:57-65 PubMed33202415

2020

The Role of Autophagy-Mediated Dengue Virus Antibody-Dependent Enhancement Infection of THP-1 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Jiang,Qiangming Sun

Cell host & microbe 26:606-622.e8 PubMed31631053

2019

Dengue NS2A Protein Orchestrates Virus Assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xuping Xie,Jing Zou,Xianwen Zhang,Yiyang Zhou,Andrew L Routh,Congbao Kang,Vsevolod L Popov,Xinwen Chen,Qing-Yin Wang,Hongping Dong,Pei-Yong Shi

Cell reports 27:2579-2592.e6 PubMed31141684

2019

Reciprocal Effects of Fibroblast Growth Factor Receptor Signaling on Dengue Virus Replication and Virion Production.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Cortese,Anil Kumar,Petr Matula,Lars Kaderali,Pietro Scaturro,Holger Erfle,Eliana Gisela Acosta,Sandra Buehler,Alessia Ruggieri,Laurent Chatel-Chaix,Karl Rohr,Ralf Bartenschlager

Virology journal 15:50 PubMed29566761

2018

The expression profile of human peripheral blood mononuclear cell miRNA is altered by antibody-dependent enhancement of infection with dengue virus serotype 3.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Jiang,Qiangming Sun

PloS one 13:e0193133 PubMed29470500

2018

Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities.

Applications

Unspecified application

Species

Unspecified reactive species

Chonticha Saisawang,Atichat Kuadkitkan,Prasert Auewarakul,Duncan R Smith,Albert J Ketterman

Scientific reports 6:22303 PubMed26923481

2016

Antibody-dependent enhancement of dengue virus infection inhibits RLR-mediated Type-I IFN-independent signalling through upregulation of cellular autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xinwei Huang,Yaofei Yue,Duo Li,Yujiao Zhao,Lijuan Qiu,Junying Chen,Yue Pan,Juemin Xi,Xiaodan Wang,Qiangming Sun,Qihan Li

Virology journal 12:150 PubMed26411318

2015

Interferon lambda inhibits dengue virus replication in epithelial cells.

Applications

ICC/IF

Species

Unspecified reactive species

Helen K Palma-Ocampo,Juan C Flores-Alonso,Verónica Vallejo-Ruiz,Julio Reyes-Leyva,Lilian Flores-Mendoza,Irma Herrera-Camacho,Nora H Rosas-Murrieta,Gerardo Santos-López

PLoS pathogens 10:e1004434 PubMed25340500

2014

Sensing of immature particles produced by dengue virus infected cells induces an antiviral response by plasmacytoid dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Elodie Décembre,Sonia Assil,Marine L B Hillaire,Wanwisa Dejnirattisai,Juthathip Mongkolsapaya,Gavin R Screaton,Andrew D Davidson,Marlène Dreux

PloS one 6:e23346 PubMed21829730

2011

Activation of Toll-like receptor 3 impairs the dengue virus serotype 2 replication through induction of IFN-β in cultured hepatoma cells.

Applications

ICC/IF

Species

Dengue virus 2

Zhaoduan Liang,Siyu Wu,Yuye Li,Li He,Minhao Wu,Lifang Jiang,Lianqiang Feng,Ping Zhang,Xi Huang
View all publications
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