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Mouse Recombinant Monoclonal Dengue Virus 1 antibody. Suitable for ICC/IF, WB and reacts with Dengue virus 3, Dengue virus 2, Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2 samples.

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Images

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390), expandable thumbnail
  • Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390), expandable thumbnail
  • Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390), expandable thumbnail

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P29991

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Dengue virus 2
Tested
Expected
Dengue virus 3
Tested
Expected
Transfected cell line - Dengue virus 2
Not recommended
Tested
Transfected cell line - Dengue virus 3
Not recommended
Tested

Tested
Tested

Species

Dengue virus 3

Dilution info

-

Notes

-

Species

Dengue virus 2

Dilution info

1/2000

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Dengue virus 3

Dilution info

-

Notes

-

Species

Transfected cell line - Dengue virus 2

Dilution info

1/5000

Notes

-

Expected
Expected

Species

Dengue virus 3, Dengue virus 2

Dilution info

Use at an assay dependent concentration.

Notes

-

Targets

Dengue Virus 2, Dengue virus 3, Dengue virus 4

Alternative names

Recommended products

Mouse Recombinant Monoclonal Dengue Virus 1 antibody. Suitable for ICC/IF, WB and reacts with Dengue virus 3, Dengue virus 2, Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2 samples.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P29991
Clone number

GT361

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Dengue Virus 2 often called Dengue Type 2 is a serotype of the dengue virus a significant vector-borne pathogen. The virus has a positive-sense single-stranded RNA genome encoding several structural and non-structural proteins including the capsid protein and NS5. The capsid protein weighing approximately 12 kDa is important for encapsulating the viral RNA forming the nucleocapsid. NS5 with a mass around 103 kDa functions as a methyltransferase and RNA-dependent RNA polymerase essential for the viral replication cycle. These proteins are expressed predominantly in host cells after the virus infects facilitating its life cycle and pathogenesis.

Biological function summary

Within host cells the capsid protein and NS5 are involved in viral assembly and RNA synthesis respectively. The capsid protein enables efficient packaging of the viral genome interacting with several cellular components for the assembly of new virions. NS5 engages in the methylation of viral RNA and replication of the viral genome. NS5 does not act alone; it works as part of a larger replication complex involving other viral proteins such as NS3 which provides helicase activity further supporting the functionality of the viral replication machinery.

Pathways

Dengue Virus 2 proteins play key roles in the viral replication and immune evasion pathways. The replication complex containing NS5 and other non-structural proteins orchestrates the replication pathway ensuring the production of viral progeny. The capsid protein is associated with the budding of new virions within the endoplasmic reticulum which is important for the release of infectious particles. In addition NS5 interacts with host proteins such as STAT2 modulating immune pathways to conceal the virus from host defenses enabling persistent viral replication and infection.

Associated diseases and disorders

Dengue Virus 2 is related to Dengue fever and severe forms like Dengue hemorrhagic fever. The NS5 protein through its interaction with STAT2 plays a role in immune suppression affecting disease outcomes. The capsid protein's strong involvement in viral assembly links it to the efficient dissemination of the virus contributing to the severity and viral load in dengue infections. Understanding these interactions is essential for future therapeutic strategies aimed at mitigating dengue-related health issues worldwide.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390), expandable thumbnail

    Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390)

    NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein by western blot analysis.
    A. 30 μg BHK-21 whole cell extract
    B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
    C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
    7.5% SDS-PAGE
    NS5 (Dengue virus 2) antibody [GT361] (ab308390) dilution: 1/5000
    The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution

    Lane 1: BHK-21 whole cell extract at 30 µg

    Lane 2: Whole cell extract of Dengue virus type 2 infected BHK-21 cells at 30 µg

    Lane 3: Whole cell extract of Dengue virus type 3 infected BHK-21 cells at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390), expandable thumbnail

    Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390)

    NS5 (Dengue Virus 2) antibody [GT361] detects NS5 protein by western blot analysis.
    A. 30 μg BHK21 whole cell lysate/extract
    B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
    C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
    10% SDS-PAGE
    NS5 (Dengue Virus 2) antibody [GT361] (ab308390) dilution: 1/5000
    The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution

    Lane 1: whole cell lysate/extract of Dengue virus 2 infected BHK21 cells at 30 µg

    Lane 2: Whole cell lysate/extract of Dengue virus 2 infected BHK21 cells at 30 µg

    Lane 3: Whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390), expandable thumbnail

    Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390)

    Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) diluted at 1/5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution

    Lane 1: Non-infected (–) C6/36 whole cell extracts at 30 µg

    Lane 2: Infected (+) C6/36 whole cell extracts at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-mouse IgG antibody

  • Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390)

    For immunofluorescence analysis, BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde and permeabilized for detection of Dengue virus Type 2 NS5 protein using ab308390 at 1/2000 dilution (green). Blue: Hoechst 33342 staining.

  • Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390)

    For immunofluorescence analysis, BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde and permeabilized for detection of Dengue virus Type 2 NS5 protein using ab308390 at 1/2000 dilution (green). Blue: Hoechst 33342 staining.

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Product protocols

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