Mouse Recombinant Monoclonal Dengue Virus 1 antibody. Suitable for ICC/IF, WB and reacts with Dengue virus 3, Dengue virus 2, Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2 samples.
IgG1
Mouse
pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
ICC/IF | WB | |
---|---|---|
Dengue virus 2 | Tested | Expected |
Dengue virus 3 | Tested | Expected |
Transfected cell line - Dengue virus 2 | Not recommended | Tested |
Transfected cell line - Dengue virus 3 | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Dengue virus 3 | Dilution info - | Notes - |
Species Dengue virus 2 | Dilution info 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2 | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Dengue virus 3 | Dilution info - | Notes - |
Species Transfected cell line - Dengue virus 2 | Dilution info 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Dengue virus 3, Dengue virus 2 | Dilution info Use at an assay dependent concentration. | Notes - |
Dengue Virus 2, Dengue virus 3, Dengue virus 4
Dengue virus type 1, Dengue virus genome polyprotein
Mouse Recombinant Monoclonal Dengue Virus 1 antibody. Suitable for ICC/IF, WB and reacts with Dengue virus 3, Dengue virus 2, Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2 samples.
IgG1
Mouse
pH: 7
Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
GT361
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
+4°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This supplementary information is collated from multiple sources and compiled automatically.
Dengue Virus 2 often called Dengue Type 2 is a serotype of the dengue virus a significant vector-borne pathogen. The virus has a positive-sense single-stranded RNA genome encoding several structural and non-structural proteins including the capsid protein and NS5. The capsid protein weighing approximately 12 kDa is important for encapsulating the viral RNA forming the nucleocapsid. NS5 with a mass around 103 kDa functions as a methyltransferase and RNA-dependent RNA polymerase essential for the viral replication cycle. These proteins are expressed predominantly in host cells after the virus infects facilitating its life cycle and pathogenesis.
Within host cells the capsid protein and NS5 are involved in viral assembly and RNA synthesis respectively. The capsid protein enables efficient packaging of the viral genome interacting with several cellular components for the assembly of new virions. NS5 engages in the methylation of viral RNA and replication of the viral genome. NS5 does not act alone; it works as part of a larger replication complex involving other viral proteins such as NS3 which provides helicase activity further supporting the functionality of the viral replication machinery.
Dengue Virus 2 proteins play key roles in the viral replication and immune evasion pathways. The replication complex containing NS5 and other non-structural proteins orchestrates the replication pathway ensuring the production of viral progeny. The capsid protein is associated with the budding of new virions within the endoplasmic reticulum which is important for the release of infectious particles. In addition NS5 interacts with host proteins such as STAT2 modulating immune pathways to conceal the virus from host defenses enabling persistent viral replication and infection.
Dengue Virus 2 is related to Dengue fever and severe forms like Dengue hemorrhagic fever. The NS5 protein through its interaction with STAT2 plays a role in immune suppression affecting disease outcomes. The capsid protein's strong involvement in viral assembly links it to the efficient dissemination of the virus contributing to the severity and viral load in dengue infections. Understanding these interactions is essential for future therapeutic strategies aimed at mitigating dengue-related health issues worldwide.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein by western blot analysis.
A. 30 μg BHK-21 whole cell extract
B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells
7.5% SDS-PAGE
NS5 (Dengue virus 2) antibody [GT361] (ab308390) dilution: 1/5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution
Lane 1: BHK-21 whole cell extract at 30 µg
Lane 2: Whole cell extract of Dengue virus type 2 infected BHK-21 cells at 30 µg
Lane 3: Whole cell extract of Dengue virus type 3 infected BHK-21 cells at 30 µg
All lanes: HRP-conjugated anti-mouse IgG antibody
NS5 (Dengue Virus 2) antibody [GT361] detects NS5 protein by western blot analysis.
A. 30 μg BHK21 whole cell lysate/extract
B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells
C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells
10% SDS-PAGE
NS5 (Dengue Virus 2) antibody [GT361] (ab308390) dilution: 1/5000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution
Lane 1: whole cell lysate/extract of Dengue virus 2 infected BHK21 cells at 30 µg
Lane 2: Whole cell lysate/extract of Dengue virus 2 infected BHK21 cells at 30 µg
Lane 3: Whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells at 30 µg
All lanes: HRP-conjugated anti-mouse IgG antibody
Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) diluted at 1/5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution
Lane 1: Non-infected (–) C6/36 whole cell extracts at 30 µg
Lane 2: Infected (+) C6/36 whole cell extracts at 30 µg
All lanes: HRP-conjugated anti-mouse IgG antibody
For immunofluorescence analysis, BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde and permeabilized for detection of Dengue virus Type 2 NS5 protein using ab308390 at 1/2000 dilution (green). Blue: Hoechst 33342 staining.
For immunofluorescence analysis, BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde and permeabilized for detection of Dengue virus Type 2 NS5 protein using ab308390 at 1/2000 dilution (green). Blue: Hoechst 33342 staining.
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