AB308390

Anti-Dengue virus Type 2 NS5 protein antibody [GT361]

Name: Anti-Dengue virus Type 2 NS5 protein antibody [GT361] - BSA and Azide free (ab308390) | Abcam
Brand: Abcam Limited
SKU: AB308390
Price: 930 USD
Availability: InStock

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Mouse Monoclonal Dengue Virus 1 antibody. Suitable for ICC/IF, WB and reacts with Dengue virus 3, Dengue virus 2, Transfected cell line - Dengue virus 3, Transfected cell line - Dengue virus 2 samples. Immunogen corresponding to Synthetic Peptide within Dengue virus type 2 (strain 16681-PDK53) POLG_DEN27.

View Alternative Names

Dengue virus type 1, Dengue virus genome polyprotein

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

For immunofluorescence analysis, BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in paraformaldehyde and permeabilized for detection of Dengue virus Type 2 NS5 protein using ab308390 at 1/2000 dilution (green). Blue : Hoechst 33342 staining.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

For immunofluorescence analysis, BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in paraformaldehyde and permeabilized for detection of Dengue virus Type 2 NS5 protein using ab308390 at 1/2000 dilution (green). Blue : Hoechst 33342 staining.

Supplier Data

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

NS5 (Dengue virus 2) antibody [GT361] detects NS5 (Dengue virus 2) protein by western blot analysis. A. 30 μg BHK-21 whole cell extract B. 30 μg whole cell extract of Dengue virus type 2 infected BHK-21 cells C. 30 μg whole cell extract of Dengue virus type 3 infected BHK-21 cells 7.5% SDS-PAGE NS5 (Dengue virus 2) antibody [GT361] (ab308390) dilution : 1/5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution

Lane 1:

BHK-21 whole cell extract at 30 µg

Lane 2:

Whole cell extract of Dengue virus type 2 infected BHK-21 cells at 30 µg

Lane 3:

Whole cell extract of Dengue virus type 3 infected BHK-21 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

false

Supplier Data

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

NS5 (Dengue Virus 2) antibody [GT361] detects NS5 protein by western blot analysis. A. 30 μg BHK21 whole cell lysate/extract B. 30 μg whole cell lysate/extract of Dengue virus 2 infected BHK21 cells C. 30 μg whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells 10% SDS-PAGE NS5 (Dengue Virus 2) antibody [GT361] (ab308390) dilution : 1/5000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution

Lane 1:

whole cell lysate/extract of Dengue virus 2 infected BHK21 cells at 30 µg

Lane 2:

Whole cell lysate/extract of Dengue virus 2 infected BHK21 cells at 30 µg

Lane 3:

Whole cell lysate/extract of Japanese encephalitis virus infected BHK21 cells at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

false

Supplier Data

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

Non-infected (–) and infected (+) C6/36 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) diluted at 1/5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (ab308390) at 1/5000 dilution

Lane 1:

Non-infected (–) C6/36 whole cell extracts at 30 µg

Lane 2:

Infected (+) C6/36 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-mouse IgG antibody

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT361

Isotype

IgG1

Carrier free

Reacts with

Dengue virus 2, Dengue virus 3

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Dengue virus type 2 (strain 16681-PDK53) POLG_DEN27. The exact immunogen used to generate this antibody is proprietary information.

P29991

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Dengue virus 2": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dengue virus 3": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Dengue virus 2": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "" }, "Transfected cell line - Dengue virus 3": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7 Constituents: 80% PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dengue Virus 2 often called Dengue Type 2 is a serotype of the dengue virus a significant vector-borne pathogen. The virus has a positive-sense single-stranded RNA genome encoding several structural and non-structural proteins including the capsid protein and NS5. The capsid protein weighing approximately 12 kDa is important for encapsulating the viral RNA forming the nucleocapsid. NS5 with a mass around 103 kDa functions as a methyltransferase and RNA-dependent RNA polymerase essential for the viral replication cycle. These proteins are expressed predominantly in host cells after the virus infects facilitating its life cycle and pathogenesis.

Biological function summary

Within host cells the capsid protein and NS5 are involved in viral assembly and RNA synthesis respectively. The capsid protein enables efficient packaging of the viral genome interacting with several cellular components for the assembly of new virions. NS5 engages in the methylation of viral RNA and replication of the viral genome. NS5 does not act alone; it works as part of a larger replication complex involving other viral proteins such as NS3 which provides helicase activity further supporting the functionality of the viral replication machinery.

Pathways

Dengue Virus 2 proteins play key roles in the viral replication and immune evasion pathways. The replication complex containing NS5 and other non-structural proteins orchestrates the replication pathway ensuring the production of viral progeny. The capsid protein is associated with the budding of new virions within the endoplasmic reticulum which is important for the release of infectious particles. In addition NS5 interacts with host proteins such as STAT2 modulating immune pathways to conceal the virus from host defenses enabling persistent viral replication and infection.

Dengue Virus 2 is related to Dengue fever and severe forms like Dengue hemorrhagic fever. The NS5 protein through its interaction with STAT2 plays a role in immune suppression affecting disease outcomes. The capsid protein's strong involvement in viral assembly links it to the efficient dissemination of the virus contributing to the severity and viral load in dengue infections. Understanding these interactions is essential for future therapeutic strategies aimed at mitigating dengue-related health issues worldwide.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Dengue Virus 1

Additional targets

,Dengue virus 3,Dengue virus 4

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dengue virus Type 2 NS5 protein antibody [GT361]

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

Immunocytochemistry/ Immunofluorescence - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

Western blot - Anti-Dengue virus Type 2 NS5 protein antibody [GT361] (AB308390)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Complementary Products

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Product promise