AB134117

Anti-DENN antibody [EPR4919]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal DENN antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 6 publications.

View Alternative Names

DENN, IG20, KIAA0358, MADD, MAP kinase-activating death domain protein, Differentially expressed in normal and neoplastic cells, Insulinoma glucagonoma clone 20, Rab3 GDP/GTP exchange factor, Rab3 GDP/GTP exchange protein, RabGEF, Rab3GEP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] (AB134117)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] (AB134117)

Immunohistochemical analysis of paraffin embedded Human breast tissue labelling DENN with ab134117 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-DENN antibody [EPR4919] (AB134117)

All lanes:

Western blot - Anti-DENN antibody [EPR4919] (ab134117) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 183 kDa

Observed band size: 220 kDa

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OI-RD Scanning - Anti-DENN antibody [EPR4919] (AB134117)

OI-RD Scanning

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OI-RD Scanning - Anti-DENN antibody [EPR4919] (AB134117)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-DENN antibody [EPR4919] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4919

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DENN domain-containing proteins also referred to as DENN serve as specific guanine nucleotide exchange factors (GEFs) that activate Rab GTPases important for vesicular trafficking. These proteins generally weigh between 110 to 150 kDa. DENN proteins predominantly are found within cellular membranes including endosomes. This positioning is consistent with their involvement in vesicle trafficking and membrane dynamics.

Biological function summary

DENN domain proteins facilitate critical processes such as endocytic recycling autophagy and transport processes within cells. These proteins belong to a complex containing multiple subunits that coordinate the activation of Rab GTPases. Through these activities DENN proteins ensure proper membrane trafficking dynamics and cellular organization integral to maintaining cell function.

Pathways

DENN proteins participate in cellular pathways like endocytic recycling and autophagy. In the endocytic pathway DENN proteins regulate Rab GTPases such as Rab5 and Rab7 controlling vesicle formation and movement. They also play a part in mTOR signaling influencing cell growth and autophagy regulation. By interacting with these key pathways DENN domain proteins help to integrate signals and coordinate cellular responses to environmental changes.

Alterations in DENN proteins often relate to neurodegenerative disorders and cancer. Mutations in the DENN domain have links to conditions such as Parkinson’s disease where Rab GTPases affected are implicated in neuronal function and survival. In cancer dysregulation of DENN protein function can influence cellular proliferation and apoptosis affecting tumor development. DENN proteins through their interactions and related pathways represent significant factors in understanding and potentially addressing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family (PubMed : 18559336, PubMed : 20937701). Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms (PubMed : 18559336, PubMed : 20937701). Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion (By similarity). Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity). Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity). Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity). Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PubMed : 32761064). May link TNFRSF1A with MAP kinase activation (PubMed : 9115275). May be involved in the regulation of TNFA-induced apoptosis (PubMed : 11577081, PubMed : 32761064).

See full target information MADD

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38512027

2024

RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Dou,Jayne Aiken,Erika L F Holzbaur

PLoS genetics 19:e1011058 PubMed38011198

2023

Accurate phenotypic classification and exome sequencing allow identification of novel genes and variants associated with adult-onset hearing loss.

Applications

Unspecified application

Species

Unspecified reactive species

Morag A Lewis,Jennifer Schulte,Lois Matthews,Kenneth I Vaden,Claire J Steves,Frances M K Williams,Bradley A Schulte,Judy R Dubno,Karen P Steel

Cell reports 42:113224 PubMed37805922

2023

Upregulation of exosome secretion from tumor-associated macrophages plays a key role in the suppression of anti-tumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqun Zhong,Youtao Lu,Xuexiang Han,Jingbo Yang,Zhiyuan Qin,Wei Zhang,Ziyan Yu,Bin Wu,Shujing Liu,Wei Xu,Cathy Zheng,Lynn M Schuchter,Giorgos C Karakousis,Tara C Mitchell,Ravi Amaravadi,Ahron J Flowers,Phyllis A Gimotty,Min Xiao,Gordon Mills,Meenhard Herlyn,Haidong Dong,Michael J Mitchell,Junhyong Kim,Xiaowei Xu,Wei Guo

Experimental neurology 353:114070 PubMed35398339

2022

Rabphilin3A reduces integrin-dependent growth cone signaling to restrict axon regeneration after trauma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Sekine,Ramakrishnan Kannan,Xingxing Wang,Stephen M Strittmatter

Brain : a journal of neurology 143:2437-2453 PubMed32761064

2020

Biallelic MADD variants cause a phenotypic spectrum ranging from developmental delay to a multisystem disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Pauline E Schneeberger,Fanny Kortüm,Georg Christoph Korenke,Malik Alawi,René Santer,Mathias Woidy,Daniela Buhas,Stephanie Fox,Jane Juusola,Majid Alfadhel,Bryn D Webb,Emanuele G Coci,Rami Abou Jamra,Manuela Siekmeyer,Saskia Biskup,Corina Heller,Esther M Maier,Poupak Javaher-Haghighi,Maria F Bedeschi,Paola F Ajmone,Maria Iascone,Hilde Peeters,Katleen Ballon,Jaak Jaeken,Aroa Rodríguez Alonso,María Palomares-Bralo,Fernando Santos-Simarro,Marije E C Meuwissen,Diane Beysen,R Frank Kooy,Henry Houlden,David Murphy,Mohammad Doosti,Ehsan G Karimiani,Majid Mojarrad,Reza Maroofian,Lenka Noskova,Stanislav Kmoch,Tomas Honzik,Heidi Cope,Amarilis Sanchez-Valle,Bruce D Gelb,Ingo Kurth,Maja Hempel,Kerstin Kutsche

The Journal of cell biology 217:3480-3496 PubMed30126838

2018

KIF1Bβ mutations detected in hereditary neuropathy impair IGF1R transport and axon growth.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Xu,Hironori Takahashi,Yosuke Tanaka,Sotaro Ichinose,Shinsuke Niwa,Matthew P Wicklund,Nobutaka Hirokawa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DENN antibody [EPR4919]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] (AB134117)

Western blot - Anti-DENN antibody [EPR4919] (AB134117)

OI-RD Scanning - Anti-DENN antibody [EPR4919] (AB134117)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise