JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248688

Anti-DENN antibody [EPR4919] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal DENN antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

DENN, IG20, KIAA0358, MADD, MAP kinase-activating death domain protein, Differentially expressed in normal and neoplastic cells, Insulinoma glucagonoma clone 20, Rab3 GDP/GTP exchange factor, Rab3 GDP/GTP exchange protein, RabGEF, Rab3GEP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] - BSA and Azide free (AB248688)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENN antibody [EPR4919] - BSA and Azide free (AB248688)

This data was developed using ab134117, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human breast tissue labelling DENN with ab134117 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-DENN antibody [EPR4919] - BSA and Azide free (AB248688)
  • WB

Unknown

Western blot - Anti-DENN antibody [EPR4919] - BSA and Azide free (AB248688)

This data was developed using ab134117, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DENN antibody [EPR4919] (<a href='/en-us/products/primary-antibodies/denn-antibody-epr4919-ab134117'>ab134117</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 183 kDa

Observed band size: 220 kDa

false

OI-RD Scanning - Anti-DENN antibody [EPR4919] - BSA and Azide free (AB248688)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-DENN antibody [EPR4919] - BSA and Azide free (AB248688)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4919

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB187070

Anti-Progranulin antibody [EPR18539-59]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Progranulin antibody [EPR18539-59] (AB187070)

  • 0
  • 0
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB111572

Anti-LRRC4C antibody

Immunocytochemistry/ Immunofluorescence - Anti-LRRC4C antibody (AB111572)

  • 4
  • 2
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column

Product details

ab248688 is the carrier-free version of ab134117.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The DENN domain-containing proteins also referred to as DENN serve as specific guanine nucleotide exchange factors (GEFs) that activate Rab GTPases important for vesicular trafficking. These proteins generally weigh between 110 to 150 kDa. DENN proteins predominantly are found within cellular membranes including endosomes. This positioning is consistent with their involvement in vesicle trafficking and membrane dynamics.
Biological function summary

DENN domain proteins facilitate critical processes such as endocytic recycling autophagy and transport processes within cells. These proteins belong to a complex containing multiple subunits that coordinate the activation of Rab GTPases. Through these activities DENN proteins ensure proper membrane trafficking dynamics and cellular organization integral to maintaining cell function.

Pathways

DENN proteins participate in cellular pathways like endocytic recycling and autophagy. In the endocytic pathway DENN proteins regulate Rab GTPases such as Rab5 and Rab7 controlling vesicle formation and movement. They also play a part in mTOR signaling influencing cell growth and autophagy regulation. By interacting with these key pathways DENN domain proteins help to integrate signals and coordinate cellular responses to environmental changes.

Alterations in DENN proteins often relate to neurodegenerative disorders and cancer. Mutations in the DENN domain have links to conditions such as Parkinson’s disease where Rab GTPases affected are implicated in neuronal function and survival. In cancer dysregulation of DENN protein function can influence cellular proliferation and apoptosis affecting tumor development. DENN proteins through their interactions and related pathways represent significant factors in understanding and potentially addressing these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Guanyl-nucleotide exchange factor that regulates small GTPases of the Rab family (PubMed : 18559336, PubMed : 20937701). Converts GDP-bound inactive form of RAB27A and RAB27B to the GTP-bound active forms (PubMed : 18559336, PubMed : 20937701). Converts GDP-bound inactive form of RAB3A, RAB3C and RAB3D to the GTP-bound active forms, GTPases involved in synaptic vesicle exocytosis and vesicle secretion (By similarity). Plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction (By similarity). Involved in up-regulating a post-docking step of synaptic exocytosis in central synapses (By similarity). Probably by binding to the motor proteins KIF1B and KIF1A, mediates motor-dependent transport of GTP-RAB3A-positive vesicles to the presynaptic nerve terminals (By similarity). Plays a role in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PubMed : 32761064). May link TNFRSF1A with MAP kinase activation (PubMed : 9115275). May be involved in the regulation of TNFA-induced apoptosis (PubMed : 11577081, PubMed : 32761064).
See full target information MADD
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 12:4513-4535 PubMed35832075

2022

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,An-Dong Liu,Qian Niu,Xiao Feng,Yuan-Yi Zheng,Shuai-Jun Chen,Hui Xu,Qian Li,Guo-Qing Hou,Xiao-Yang Bi,Yu-Zhi Lu,Pei-Pei Cheng,Li-Mei Liang,Ye-Han Jiang,Li-Qin Zhao,Fei Liu,Lin-Jie Song,Li-Ling Zhou,Ling-Yan Xiao,Feng Chen,Shawn Shun-Cheng Li,Wan-Li Ma,Xuan Cao,Hong Ye
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com