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Rabbit Recombinant Monoclonal Density Regulated Protein antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (AB324269), expandable thumbnail
  • Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (AB324269), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Target data

Function

Translation regulator forming a complex with MCTS1 to promote translation reinitiation. Translation reinitiation is the process where the small ribosomal subunit remains attached to the mRNA following termination of translation of a regulatory upstream ORF (uORF), and resume scanning on the same mRNA molecule to initiate translation of a downstream ORF, usually the main ORF (mORF). The MCTS1/DENR complex is pivotal to two linked mechanisms essential for translation reinitiation. Firstly, the dissociation of deacylated tRNAs from post-termination 40S ribosomal complexes during ribosome recycling. Secondly, the recruitment in an EIF2-independent manner of aminoacylated initiator tRNA to P site of 40S ribosomes for a new round of translation. This regulatory mechanism governs the translation of more than 150 genes which translation reinitiation is MCTS1/DENR complex-dependent.

Alternative names

DRP1, H14, DENR, Density-regulated protein, DRP, Protein DRP1, Smooth muscle cell-associated protein 3, SMAP-3

Rabbit Recombinant Monoclonal Density Regulated Protein antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29751-532
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (ab324269), expandable thumbnail

    Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (ab324269)

    Density Regulated Protein Western blot staining using rabbit Anti-Density Regulated Protein antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-4: 48 seconds, lanes 5-10: 180 seconds

    All lanes: Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (ab324269) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Lanes 3 - 4: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 5: MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

    Lane 6: P815 (mouse mastocytoma mast Cell) whole cell lysate at 20 µg

    Lane 7: F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 8: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 9: Mouse heart tissue lysate at 20 µg

    Lane 10: Mouse kidney tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 26 kDa, 36 kDa

  • Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (ab324269), expandable thumbnail

    Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (ab324269)

    Density Regulated Protein Western blot staining using rabbit Anti-Density Regulated Protein antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HL-60, IMR-90.

    The identity of the band at approximately 50 kDa (in lanes 4-5, 7-8) is unknown.

    The identity of the bands between 15 kDa and 25 kDa (in lane 7) are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Density Regulated Protein antibody [EPR29751-532] (ab324269) at 1/1000 dilution

    Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 2: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 3: Calu-1 (human lung epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 5: PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg

    Lane 7: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg

    Lane 8: IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 26 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com