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AB168860

Anti-DENTT antibody

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(2 Publications)

Rabbit Polyclonal DENTT antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TSPYL2 aa 600 to C-terminus.

View Alternative Names

CDA1, DENTT, TSPX, HRIHFB2216, TSPYL2, Testis-specific Y-encoded-like protein 2, TSPY-like protein 2, Cell division autoantigen 1, Cutaneous T-cell lymphoma-associated antigen se20-4, Differentially-expressed nucleolar TGF-beta1 target protein, Nuclear protein of 79 kDa, CTCL-associated antigen se20-4, NP79

1 Images
Western blot - Anti-DENTT antibody (AB168860)
  • WB

Unknown

Western blot - Anti-DENTT antibody (AB168860)

All lanes:

Western blot - Anti-DENTT antibody (ab168860) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TSPYL2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9H2G4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab168860 was affinity purified using an epitope specific to DENTT immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DENTT also known as DEP domain-containing protein 5 (DEPDC5) is a member of the GATOR complex. It has a molecular mass of approximately 160 kDa. DENTT is expressed in various human tissues with high levels in the brain and skeletal muscle. This protein plays a role in the mechanisms of nutrient sensing and detecting energy levels within cells.
Biological function summary

DENTT regulates the activity of the mechanistic target of rapamycin complex 1 (mTORC1). As part of the GATOR1 complex it serves to inhibit mTORC1 in response to nutrient levels. By doing so DENTT participates in cellular processes such as growth and metabolism. Its activity is linked to the control of cell size and autophagy.

Pathways

DENTT is involved in the mTOR signaling pathway an important regulator of cell growth and proliferation. It interacts with proteins such as NPRL2 and NPRL3 which form part of the same GATOR1 complex. The interplay between DENTT and these proteins modulates mTORC1 activity aligning cellular metabolism with nutrient availability.

Alterations in DENTT are implicated in familial focal epilepsies. Mutations within the DEPDC5 gene can disturb mTOR signaling leading to imbalanced neuronal excitability. Additionally aberrant DENTT activity connects with proteins like RHEB known to drive mTORC1 activity which contributes to the pathogenesis of certain epileptic conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.
See full target information TSPYL2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer gene therapy 30:608-621 PubMed36494579

2022

FAP is critical for ovarian cancer cell survival by sustaining NF-κB activation through recruitment of PRKDC in lipid rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Li,Zuo Ding,Ozlem Calbay,Yue Li,Tao Li,Lingtao Jin,Shuang Huang

Scientific reports 10:12716 PubMed32728041

2020

Development of the FAST-DOSE assay system for high-throughput biodosimetry and radiation triage.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wang,Younghyun Lee,Igor Shuryak,Monica Pujol Canadell,Maria Taveras,Jay R Perrier,Bezalel A Bacon,Matthew A Rodrigues,Richard Kowalski,Christopher Capaccio,David J Brenner,Helen C Turner
View all publications
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