AB240596

Anti-DENTT antibody

Name: Anti-DENTT antibody (ab240596) | Abcam
Brand: Abcam Limited
SKU: AB240596

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(3 Publications)

Rabbit Polyclonal DENTT antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TSPYL2 aa 1-50.

View Alternative Names

CDA1, DENTT, TSPX, HRIHFB2216, TSPYL2, Testis-specific Y-encoded-like protein 2, TSPY-like protein 2, Cell division autoantigen 1, Cutaneous T-cell lymphoma-associated antigen se20-4, Differentially-expressed nucleolar TGF-beta1 target protein, Nuclear protein of 79 kDa, CTCL-associated antigen se20-4, NP79

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for DENTT using ab240596 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Formalin-fixed, paraffin-embedded human testis tissue stained for DENTT using ab240596 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Immunoprecipitation - Anti-DENTT antibody (AB240596)

DENTT was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab240596 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab240596 at 1 μg/mL.

Lane 1 : ab240596 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DENTT antibody (ab240596)

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-DENTT antibody (AB240596)

All lanes:

Western blot - Anti-DENTT antibody (ab240596) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TSPYL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H2G4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DENTT also known as DEP domain-containing protein 5 (DEPDC5) is a member of the GATOR complex. It has a molecular mass of approximately 160 kDa. DENTT is expressed in various human tissues with high levels in the brain and skeletal muscle. This protein plays a role in the mechanisms of nutrient sensing and detecting energy levels within cells.

Biological function summary

DENTT regulates the activity of the mechanistic target of rapamycin complex 1 (mTORC1). As part of the GATOR1 complex it serves to inhibit mTORC1 in response to nutrient levels. By doing so DENTT participates in cellular processes such as growth and metabolism. Its activity is linked to the control of cell size and autophagy.

Pathways

DENTT is involved in the mTOR signaling pathway an important regulator of cell growth and proliferation. It interacts with proteins such as NPRL2 and NPRL3 which form part of the same GATOR1 complex. The interplay between DENTT and these proteins modulates mTORC1 activity aligning cellular metabolism with nutrient availability.

Alterations in DENTT are implicated in familial focal epilepsies. Mutations within the DEPDC5 gene can disturb mTOR signaling leading to imbalanced neuronal excitability. Additionally aberrant DENTT activity connects with proteins like RHEB known to drive mTORC1 activity which contributes to the pathogenesis of certain epileptic conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.

See full target information TSPYL2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:271 PubMed40210616

2025

Role of METTL16 in PPARγ methylation and osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Liangjie Lu,Lijun Wang,Minjie Yang,Huihan Wang

Heliyon 10:e39260 PubMed39669138

2024

Systematic exploration of the molecular characteristics of CD8 T cells to predict the response to immunotherapy and the prognosis of patients with colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Shen,Lifeng Shang,Junwei Han,Yi Zhang,Wenkai Niu,Haiwang Liu,Hai Shi

Frontiers in immunology 14:1077003 PubMed37033917

2023

Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Guang,Zhang Min,Li Jun-Tan,Dou Tian-Xu,Gao Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DENTT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Immunoprecipitation - Anti-DENTT antibody (AB240596)

Western blot - Anti-DENTT antibody (AB240596)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise