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AB250457

Anti-DEP1 antibody [EPR13398] - BSA and Azide free

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Rabbit Recombinant Monoclonal DEP1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CD148, DEP1, PTPRJ, Receptor-type tyrosine-protein phosphatase eta, Protein-tyrosine phosphatase eta, R-PTP-eta, Density-enhanced phosphatase 1, HPTP eta, Protein-tyrosine phosphatase receptor type J, DEP-1, R-PTP-J

1 Images
Western blot - Anti-DEP1 antibody [EPR13398] - BSA and Azide free (AB250457)
  • WB

Supplier Data

Western blot - Anti-DEP1 antibody [EPR13398] - BSA and Azide free (AB250457)

This data was developed using ab181244, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DEP1 antibody [EPR13398] (<a href='/en-us/products/primary-antibodies/dep1-antibody-epr13398-ab181244'>ab181244</a>) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 145 kDa

Observed band size: 220-250 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13398

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250457 is the carrier-free version of ab181244.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEP1 also known as protein tyrosine phosphatase receptor type J (PTPRJ) is an important regulator in cell signaling. Its molecular mass is approximately 135-150 kDa. DEP1 is a transmembrane protein expressed in many tissues like epithelial cells endothelial cells and hematopoietic cells. It functions as a protein tyrosine phosphatase meaning it removes phosphate groups from tyrosine residues on target proteins which impacts many cellular processes.
Biological function summary

DEP1 acts as a negative regulator of cell proliferation differentiation and migration. It plays a role in maintaining cellular homeostasis and is often found in complex with other signaling molecules at cell-cell junctions. DEP1 modulates signaling pathways by dephosphorylating receptor tyrosine kinases or their downstream targets. This regulation impacts how cells respond to external stimuli and maintain normal physiological activities.

Pathways

DEP1 is active in both the RAS/MAPK signaling pathway and the PI3K/AKT pathway. It interacts with signaling molecules such as epidermal growth factor receptor (EGFR) and vascular endothelial growth factor receptor (VEGFR) impacting their signaling abilities. By dephosphorylating these receptors DEP1 modulates the pathways that control cell growth and survival influencing how cells react to growth factors and other signals.

DEP1 connects to several cancers and cardiovascular diseases. It often acts as a tumor suppressor by inhibiting signaling pathways that promote uncontrolled cell growth. In cancer altered DEP1 expression or function can lead to changes in cellular behaviors contributing to tumorigenesis. It relates to proteins like VEGFR in these contexts as their dysregulation can lead to abnormal vascularization in tumors and in cardiovascular disease affecting angiogenesis and blood vessel integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2 (PubMed : 10821867, PubMed : 12062403, PubMed : 12370829, PubMed : 12475979, PubMed : 18348712, PubMed : 19494114, PubMed : 19922411, PubMed : 21262971). Plays a role in cell adhesion, migration, proliferation and differentiation (PubMed : 12370829, PubMed : 14709717, PubMed : 16682945, PubMed : 19836242). Has a role in megakaryocytes and platelet formation (PubMed : 30591527). Involved in vascular development (By similarity). Regulator of macrophage adhesion and spreading (By similarity). Positively affects cell-matrix adhesion (By similarity). Positive regulator of platelet activation and thrombosis. Negative regulator of cell proliferation (PubMed : 16682945). Negative regulator of PDGF-stimulated cell migration; through dephosphorylation of PDGFR (PubMed : 21091576). Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation; through KDR dephosphorylation (PubMed : 18936167). Negative regulator of EGFR signaling pathway; through EGFR dephosphorylation (PubMed : 19836242). Enhances the barrier function of epithelial junctions during reassembly (PubMed : 19332538). Negatively regulates T-cell receptor (TCR) signaling (PubMed : 11259588, PubMed : 9531590, PubMed : 9780142). Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling (PubMed : 11259588, PubMed : 12913111).. Isoform 2. Activates angiogenesis and cell migration (PubMed : 28052032). Downregulates the expression of the endothelial adhesion molecules ICAM1 and VCAM1 (PubMed : 28052032).
See full target information PTPRJ
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Product promise

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