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AB197246

Anti-DEPDC1 antibody

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(7 Publications)

Rabbit Polyclonal DEPDC1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DEPDC1 aa 1-250.

View Alternative Names

DEPDC1A, DEPDC1, DEP domain-containing protein 1A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1 antibody (AB197246)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1 antibody (AB197246)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human fetal pancreas tissue labeling DEPDC1 with ab197246 at a 1/100 dilution.

Western blot - Anti-DEPDC1 antibody (AB197246)
  • WB

Supplier Data

Western blot - Anti-DEPDC1 antibody (AB197246)

All lanes:

Western blot - Anti-DEPDC1 antibody (ab197246) at 1/500 dilution

All lanes:

HeLa lysate

Predicted band size: 93 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DEPDC1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q5TB30

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 200µL
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEPDC1 also known as DEP domain-containing protein 1 has a molecular weight of roughly 92 kDa. It primarily functions as a nuclear protein playing roles in cell cycle regulation and mitosis. DEPDC1 is found expressed in various tissues but its expression is notably high in certain cancer cells. This protein interacts with other cellular components within the nucleus indicating its involvement in important cellular processes related to proliferation.
Biological function summary

DEPDC1 influences cell growth and division. It participates in the formation of complexes with other proteins which are essential for its role in mitotic progression. Through its interactions in these complexes DEPDC1 regulates key processes needed for proper cell cycle check and balance ensuring cells divide correctly. Moreover its levels can affect how tissues develop and regenerate considering its heightened activity during rapid cell division phases.

Pathways

The function of DEPDC1 ties into significant cellular mechanisms like the cell cycle and Wnt signaling pathways. In the cell cycle DEPDC1 associates with various cyclins and cyclin-dependent kinases to modulate transition between different phases facilitating smooth progression through mitotic checkpoints. Furthermore in the Wnt signaling pathway it may impact how signals are transduced influencing cellular differentiation and development processes potentially interacting with proteins such as beta-catenin.

DEPDC1 has clear associations with cancer and neurodegenerative diseases. In cancer its overexpression links to the uncontrolled cell growth seen in tumors interacting with proteins like p53 which are responsible for cell cycle arrest and apoptosis. In some neurodegenerative diseases irregularities in DEPDC1 functions can lead to improper neuronal cell cycle re-entry causing neurodegeneration. Its involvement in these pathologies makes it a notable target for research into therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
See full target information DEPDC1
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 16:1591390 PubMed40600015

2025

DEPDC1 facilitated malignant phenotypes and disease progression of liposarcoma by modulating KIF20A.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwei Yu,Huishan Zhao,Yujie Sun

Oncology letters 29:276 PubMed40247989

2025

DEP domain‑containing 1 is a prognostic biomarker associated with the cell cycle in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Haima Li,Yezu Liu,Hanwen Zhang,Xuelian Wang

Annals of translational medicine 10:1355 PubMed36660720

2023

Pan-cancer analysis of as a candidate prognostic biomarker and associated with immune infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Boquan Jia,Jun Liu,Xin Hu,Lu Xia,Ying Han

Molecular medicine reports 26: PubMed35795985

2022

FOXO3a‑modulated DEPDC1 promotes malignant progression of nephroblastoma via the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Geng,Qinghao Li,Xingqing Guo,Qingbin Ni,Yongtao Xu,Zhaolong Ma,Yongjin Wang,Ming Ming

Zhongguo fei ai za zhi = Chinese journal of lung cancer 24:453-460 PubMed34134185

2021

[DEPDC1 is Highly Expressed in Lung Adenocarcinoma and Promotes Tumor Cell Proliferation].

Applications

Unspecified application

Species

Unspecified reactive species

Jian Shen,Mengmeng Xi

Anticancer research 39:4423-4430 PubMed31366540

2019

Prognostic Value of DEPDC1 Expression in Tumor and Non-tumor Tissue of Patients With Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Masataka Amisaki,Takuki Yagyu,E I Uchinaka,Masaki Morimoto,Takehiko Hanaki,Joji Watanabe,Naruo Tokuyasu,Teruhisa Sakamoto,Soichiro Honjo,Yoshiyuki Fujiwara

Bioscience reports 39: PubMed31189746

2019

DEPDC1 promotes cell proliferation and suppresses sensitivity to chemotherapy in human hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zhou,Pu Wang,Mengtian Tu,Yi Huang,Fei Xiong,Yue Wu
View all publications
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