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AB237542

Anti-DEPDC1B antibody

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(2 Publications)

Rabbit Polyclonal DEPDC1B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DEPDC1B aa 300 to C-terminus.

View Alternative Names

XTP8, DEPDC1B, DEP domain-containing protein 1B, HBV X-transactivated gene 8 protein, HBV XAg-transactivated protein 8

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1B antibody (AB237542)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1B antibody (AB237542)

Paraffin-embedded human liver cancer tissue stained for DEPDC1B using ab237542 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1B antibody (AB237542)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DEPDC1B antibody (AB237542)

Paraffin-embedded human testis tissue stained for DEPDC1B using ab237542 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DEPDC1B antibody (AB237542)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DEPDC1B antibody (AB237542)

HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for DEPDC1B (green) using ab237542 at 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DEPDC1B aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WUY9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEP domain-containing protein 1B (DEPDC1B) is a protein that plays a role in the regulation of cell cycle and cytoskeletal dynamics. Also known as DEP.13 this protein has a molecular mass of approximately 70 kDa. DEPDC1B is expressed in various tissues with notably higher expression levels in testis and prostate tissues. This protein is characterized by the presence of a RhoGAP domain which suggests its involvement in modulating Rho family GTPases influencing cytoskeletal organization.
Biological function summary

DEPDC1B is involved in cell migration and mitosis. It operates as part of a larger protein complex that interacts with other regulatory proteins. Its interaction with RhoA and Cdc42 highlights its role in promoting changes in cell morphology and motility. DEPDC1B contributes to cell cycle progression especially during the transition from G1 to S phase indicating its importance in cell proliferation and growth.

Pathways

DEPDC1B is significant in the Rho GTPase signaling pathway and the cell cycle regulation pathway. Its function in these pathways includes co-regulating the activity of proteins like ROCK and MLK which are pivotal for cytoskeletal rearrangement and cell division. DEPDC1B's modulation of these pathways highlights its role in maintaining cellular architecture and ensuring proper cell cycle checkpoints.

Dysfunctions in DEPDC1B have been associated with cancer and neurological disorders. Studies show altered expression of DEPDC1B in certain cancers including prostate cancer linking it to tumor progression and metastasis through its interaction with proteins like RhoA. Additionally abnormalities in its activity are implicated in neurodevelopmental disorders connecting it to proteins influencing neuronal signaling pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information DEPDC1B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 15:4171-4184 PubMed35912402

2022

High Expression of DEPDC1B Predicts Poor Prognosis in Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Pulin Li,Xiaojuan Chen,Sijing Zhou,Xingyuan Xia,Enze Wang,Rui Han,Daxiong Zeng,Guanghe Fei,Ran Wang

Biochimica et biophysica acta. Molecular basis of disease 1868:166476 PubMed35811030

2022

Butyrate oxidation attenuates the butyrate-induced improvement of insulin sensitivity in myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Melany Rios-Morales,Marcel A Vieira-Lara,Esther Homan,Miriam Langelaar-Makkinje,Albert Gerding,Zhuang Li,Nicolette Huijkman,Patrick C N Rensen,Justina C Wolters,Dirk-Jan Reijngoud,Barbara M Bakker
View all publications
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