AB213181

Anti-DEPDC5 antibody [EPR20497-23]

Name: Anti-DEPDC5 antibody [EPR20497-23] (ab213181) | Abcam
Brand: Abcam Limited
SKU: AB213181
Price: 5700 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
KO Validated
20ul selling size
What is this?

(2 Reviews)

(10 Publications)

Anti-DEPDC5 antibody [EPR20497-23] (ab213181) is a rabbit monoclonal antibody detecting DEPDC5 in Western Blot. Suitable for Human, Mouse.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

KIAA0645, DEPDC5, GATOR1 complex protein DEPDC5, DEP domain-containing protein 5

2 Images

Lab

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)

False colour image of Western blot : Anti-DEPDC5 antibody [EPR20497-23] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab213181 was shown to bind specifically to DEPDC5. A band was observed at 181 kDa in wild-type A549 cell lysates with no signal observed at this size in DEPDC5 knockout cell line ab266906 (knockout cell lysate ab258394). To generate this image, wild-type and DEPDC5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

DEPDC5 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human DEPDC5 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-depdc5-knockout-a549-cell-line-ab266906'>ab266906</a>)

Lane 3:

Human brain tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Predicted band size: 178 kDa

Observed band size: 181 kDa

false

Lab

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The wild-type and DEPDC5 knockout mouse E14 brain tissue lysates were kindly provided by an anonymous collaborator.

ab213181 was shown to specifically react with DEPDC5 in wild-type mouse E14 brain tissue as signal was lost in DEPDC5 knockout tissue. Wild-type and DEPDC5 knockout samples were subjected to SDS-PAGE. ab213181 and ab129002 (Rabbit anti-Vinculin loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/5000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100, 000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.

Exposure time : 26 seconds.

All lanes:

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (ab213181) at 1/1000 dilution

Lane 1:

Wild-type mouse E14 brain tissue lysate at 20 µg

Lane 2:

DEPDC5 knockout mouse E14 brain tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 178 kDa

Observed band size: 181 kDa

false

Carrier free

Anti-DEPDC5 antibody [EPR20497-23] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20497-23

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-DEPDC5 antibody [EPR20497-23] (ab213181) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples.

What is the molecular weight of DEPDC5?
Anti-DEPDC5 [EPR20497-23] (ab213181) specifically detects a band for DEPDC5 (UniProt: O75140) at a molecular weight of 181kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-DEPDC5 antibody [EPR20497-23] (ab213181) has been confirmed by Western blot testing in DEPDC5 Knockout A549 cell line, ab266906.

Other related products
We have a range of other formats of antibody clone [EPR20497-23] also available for your convenience: ab213181, Carrier free - ab272399

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEPDC5 also called DEP domain containing 5 is a protein that plays a significant role in various cellular processes. It belongs to a protein family known for its involvement in signal transduction. DEPDC5 has a mass of about 160 kDa. This protein expresses broadly in many tissues with notable presence in the brain and the liver indicating its potential importance in these organs.

Biological function summary

DEPDC5 acts as a negative regulator of the mTORC1 signaling pathway by being a part of the GATOR1 complex. This complex is involved in nutrient sensing and regulation of cell growth and metabolism. DEPDC5's role in mTORC1 regulation links it to the control of protein synthesis and autophagy essential processes in maintaining cellular homeostasis. By interacting with other components of the GATOR1 complex DEPDC5 contributes not only to inhibiting mTORC1 activity but also to the broader metabolic balance.

Pathways

DEPDC5 is integral to the mTOR signaling pathway and the amino acid sensing pathways. The protein acts in coordination with other GATOR1 complex components such as NPRL2 and NPRL3 which together monitor nutrient availability and adapt cellular functions accordingly. These pathways highlight DEPDC5's role in connecting nutrient signals to cellular growth responses showing its influence on critical regulatory networks affecting cell growth and proliferation.

Mutations in DEPDC5 have been linked to certain neurological disorders notably focal epilepsy. The protein's involvement in the brain points to its connection with proper neural function where its disruption may lead to disease. Researchers have also found related proteins like MTOR and NPRL3 being associated in these epilepsy cases emphasizing the importance of DEPDC5 in neural signaling pathways and its potential as a target for therapeutic approaches.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 29590090, PubMed : 29769719, PubMed : 31548394, PubMed : 35338845). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (PubMed : 23723238, PubMed : 25457612, PubMed : 29590090, PubMed : 29769719, PubMed : 35338845). In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 29769719). Within the GATOR1 complex, DEPDC5 mediates direct interaction with the nucleotide-binding pocket of small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) and coordinates their nucleotide loading states by promoting RagA/RRAGA or RagB/RRAGB into their GDP-binding state and RagC/RRAGC or RagD/RRAGD into their GTP-binding state (PubMed : 29590090, PubMed : 35338845). However, it does not execute the GAP activity, which is mediated by NPRL2 (PubMed : 29590090).

See full target information DEPDC5

style

left-column

Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature 646:493-500 PubMed40836086

2025

Structural basis for the dynamic regulation of mTORC1 by amino acids.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Maximilian Wranik,Pranav V Lalgudi,Karen Y Linde-Garelli,Yuri Choi,Raghu R Chivukula,David M Sabatini,Kacper B Rogala

Proceedings of the National Academy of Sciences of the United States of America 121:e2322755121 PubMed39163330

2024

Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Pranav V Lalgudi,Xin Gu,Jibril F Kedir,Martin S Taylor,Raghu R Chivukula,David M Sabatini

Nature communications 15:3789 PubMed38710693

2024

A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3.

Applications

Species

Human

Weize Wang,Ling Liang,Zonglin Dai,Peng Zuo,Shang Yu,Yishuo Lu,Dian Ding,Hongyi Chen,Hui Shan,Yan Jin,Youdong Mao,Yuxin Yin

Nature communications 14:8464 PubMed38123554

2023

VWCE modulates amino acid-dependent mTOR signaling and coordinates with KICSTOR to recruit GATOR1 to the lysosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Zhao,Yuanyuan Guan,Chenchen Xu,Dong Wang,Jialiang Guan,Ying Liu

Nature 620:200-208 PubMed37407815

2023

SLC38A2 and glutamine signalling in cDC1s dictate anti-tumour immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Chuansheng Guo,Zhiyuan You,Hao Shi,Yu Sun,Xingrong Du,Gustavo Palacios,Cliff Guy,Sujing Yuan,Nicole M Chapman,Seon Ah Lim,Xiang Sun,Jordy Saravia,Sherri Rankin,Yogesh Dhungana,Hongbo Chi

Nature cell biology 25:754-764 PubMed37037994

2023

Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.

Applications

Species

Human

Guokai Yan,Jinxin Yang,Wen Li,Ao Guo,Jialiang Guan,Ying Liu

Phytomedicine : international journal of phytotherapy and phytopharmacology 111:154677 PubMed36724620

2023

α-hederin regulates glucose metabolism in intestinal epithelial cells by increasing SNX10 expression.

Applications

Species

Human

Hui Feng,Jiani Tan,Qijuan Wang,Tingting Zhou,Liu Li,Dongdong Sun,Minmin Fan,Haibo Cheng,Weixing Shen

Frontiers in cardiovascular medicine 9:1003282 PubMed36172581

2022

Exosomes derived from pericardial adipose tissues attenuate cardiac remodeling following myocardial infarction by Adipsin-regulated iron homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wanrong Man,Xinglong Song,Zhenyu Xiong,Jing Gu,Jie Lin,Xiaoming Gu,Duan Yu,Congye Li,Mengyuan Jiang,Xuebin Zhang,Zhi Yang,Yang Cao,Yan Zhang,Xiaofei Shu,Dexi Wu,Haichang Wang,Gang Ji,Dongdong Sun

Nature 607:610-616 PubMed35831510

2022

Structure of the nutrient-sensing hub GATOR2.

Applications

Unspecified application

Species

Unspecified reactive species

Max L Valenstein,Kacper B Rogala,Pranav V Lalgudi,Edward J Brignole,Xin Gu,Robert A Saxton,Lynne Chantranupong,Jonas Kolibius,Jan-Philipp Quast,David M Sabatini

Biochemical and biophysical research communications 569:118-124 PubMed34243067

2021

Adipocyte-specific deletion of Depdc5 exacerbates insulin resistance and adipose tissue inflammation in mice.

Applications

Species

Mouse

Jinghong Zhang,Yiping Shi,Chenyan Yang,Bo Sun,Honghui Ma,Rong Huang,Zun Li,Mingyong Wang,Qingzhi Wang,Jie Ma,Xiwen Xiong

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DEPDC5 antibody [EPR20497-23]

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)

Western blot - Anti-DEPDC5 antibody [EPR20497-23] (AB213181)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise