AB191841

Anti-Deptor antibody

Name: Anti-Deptor antibody (ab191841) | Abcam
Brand: Abcam Limited
SKU: AB191841
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Deptor antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DEPTOR.

View Alternative Names

DEPDC6, DEPTOR, DEP domain-containing mTOR-interacting protein, hDEPTOR, DEP domain-containing protein 6

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Deptor antibody (AB191841)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Deptor antibody (AB191841)

Immunohistochemical analysis of mouse liver tissue labeling Deptor with ab191841 at 5 μg/ml.

Supplier Data

Western blot - Anti-Deptor antibody (AB191841)

Lane 1:

Western blot - Anti-Deptor antibody (ab191841) at 1 µg/mL

Lane 2:

Western blot - Anti-Deptor antibody (ab191841) at 2 µg/mL

All lanes:

Mouse liver tissue lysate at 15 µg

Predicted band size: 46 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DEPTOR. The exact immunogen used to generate this antibody is proprietary information.

Q8TB45

Specificity

At least three isoforms of Deptor are known to exist; ab191841 will detect all three isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

DEPTOR also known as DEP domain-containing mTOR-interacting protein functions as a subunit of the mTOR (mechanistic Target of Rapamycin) complexes. This protein has a molecular weight of approximately 48 kDa and is expressed extensively in tissues such as skeletal muscle adipose tissue and the brain. DEPTOR directly interacts with the mTOR complexes modulating their activity. Its exact mechanical role involves binding to mTOR and influencing the assembly and function of mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2).

Biological function summary

DEPTOR is important for the regulation of cell growth and metabolism. It is a component of the mTORC1 and mTORC2 two key protein complexes that control cell proliferation survival and autophagy. DEPTOR acts as an inhibitor within these complexes reducing their activity. By regulating the mTOR signaling pathway it helps balance anabolic and catabolic processes within the cell. Without proper DEPTOR function cells may grow unchecked leading to various issues including metabolic dysregulation.

Pathways

DEPTOR plays a significant role in the mTOR signaling pathway and the PI3K/AKT pathway. In the mTOR pathway DEPTOR controls the activity of mTORC1 and mTORC2 which are essential for translating signaling into cellular response. The PI3K/AKT pathway also involves DEPTOR as it relates to insulin signaling and glucose homeostasis. Proteins such as AKT and S6K work in this complex to transmit signals impacting numerous cellular activities. The dynamic balance it maintains within these pathways is critical for normal cellular function and metabolism.

DEPTOR is associated with conditions such as cancer and type 2 diabetes. In cancers its reduced expression allows for unchecked mTOR activity driving tumor growth and survival. Conversely unregulated expression can lead to increased cell apoptosis. DEPTOR's dysregulation in the context of type 2 diabetes affects glucose metabolism through interactions with the PI3K/AKT pathway. It also interacts with proteins like IRS-1 influencing insulin sensitivity and leading to metabolic disorders. DEPTOR's regulation of these pathways suggests potential therapeutic targets in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Negative regulator of the mTORC1 and mTORC2 complexes : inhibits the protein kinase activity of MTOR, thereby inactivating both complexes (PubMed : 19446321, PubMed : 22017875, PubMed : 22017876, PubMed : 22017877, PubMed : 25936805, PubMed : 29382726, PubMed : 34519268, PubMed : 34519269). DEPTOR inhibits mTORC1 and mTORC2 to induce autophagy (PubMed : 22017875, PubMed : 22017876, PubMed : 22017877). In contrast to AKT1S1/PRAS40, only partially inhibits mTORC1 activity (PubMed : 34519268, PubMed : 34519269).

See full target information DEPTOR

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Advanced genetics (Hoboken, N.J.) 3:2100060 PubMed36618022

2022

Single-Cell Transcriptome Identifies Drug-Resistance Signature and Immunosuppressive Microenvironment in Metastatic Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Haiping Zhang,Lele Zhang,Dianke Li,Mengfan Qi,Liping Zhang,Huansha Yu,Di Wang,Gening Jiang,Xujun Wang,Xianmin Zhu,Peng Zhang

Animal nutrition (Zhongguo xu mu shou yi xue hui) 10:12-18 PubMed35601254

2022

The ubiquitin ligase Nedd4-2 mediates the regulation of PepT2 by mTORC1 in bovine mammary epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Caihong Wang,Fengqi Zhao,Jianxin Liu,Hongyun Liu

BMC genomics 22:892 PubMed34903175

2021

The effect of lipocalin-2 (LCN2) on apoptosis: a proteomics analysis study in an LCN2 deficient mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Dongming Wu,Xiaopeng Wang,Ye Han,Yayun Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Deptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Deptor antibody (AB191841)

Western blot - Anti-Deptor antibody (AB191841)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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