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AB176732

Anti-DERL1/Derlin-1 antibody

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(14 Publications)

Rabbit Polyclonal DERL1/Derlin-1 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human DERL1 aa 200 to C-terminus.

View Alternative Names

DER1, UNQ243/PRO276, DERL1, Derlin-1, Degradation in endoplasmic reticulum protein 1, Der1-like protein 1, DERtrin-1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DERL1/Derlin-1 antibody (AB176732)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DERL1/Derlin-1 antibody (AB176732)

Immunohistochemical analysis of formalin fixed, paraffin embedded Human ovarian carcinoma tissue labeling DERL1/Derlin-1 using ab176732 at a 1/200 dilution, followed by peroxidase substrate detection.

Immunoprecipitation - Anti-DERL1/Derlin-1 antibody (AB176732)
  • IP

Supplier Data

Immunoprecipitation - Anti-DERL1/Derlin-1 antibody (AB176732)

ab176732 at 0.4 μg/ml detecting DERL1/Derlin-1 in HeLa whole cell lysate by WB following IP. ab176732 was used for IP at 3 μg/mg of lysate, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-DERL1/Derlin-1 antibody (ab176732)

Predicted band size: 29 kDa

false

Western blot - Anti-DERL1/Derlin-1 antibody (AB176732)
  • WB

Supplier Data

Western blot - Anti-DERL1/Derlin-1 antibody (AB176732)

All lanes:

Western blot - Anti-DERL1/Derlin-1 antibody (ab176732) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 29 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human DERL1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BUN8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab176732 is affinity purified using an epitope specific to DERL1/Derlin-1 immobilized on solid support.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Derlin-1 (also known as DERL1 or Derlin-1) is a protein with an approximate mass of 27 kDa. It plays an important role in the endoplasmic reticulum-associated degradation (ERAD) pathway. This protein is involved in the retro-translocation process where misfolded proteins are moved from the endoplasmic reticulum to the cytosol for degradation by the proteasome. DERL1 typically expresses in various tissues with higher levels in secretory cells reflecting its role in protein quality control.
Biological function summary

Derlin-1 helps maintain cellular protein homeostasis and acts as a channel or gatekeeper for misfolded or unfolded proteins. It associates with other ERAD components to form a larger degradation complex. Derlin-1 interacts with proteins such as VCP/p97 and the ubiquitin ligase HRD1 which collaborate to tag defective proteins with ubiquitin preparing them for degradation.

Pathways

The ERAD pathway which Derlin-1 is part of is important for the removal of misfolded proteins protecting cells from stress caused by protein accumulation. Derlin-1's interaction with VCP/p97 and other proteins within the ERAD pathway highlights its function in proteostasis. This process is essential to the Unfolded Protein Response (UPR) another pathway that senses and reacts to misfolded proteins in the endoplasmic reticulum.

Derlin-1's function links to conditions such as cystic fibrosis and certain neurodegenerative diseases. Its role in protein misfolding and degradation relates to these diseases by causing an accumulation of defective proteins. Within the context of cystic fibrosis Derlin-1's role becomes critical in managing faulty CFTR protein degradation. Its interactions with proteins like VCP/p97 can modulate the pathogenesis of neurodegenerative diseases where misfolded protein aggregates play a significant role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functional component of endoplasmic reticulum-associated degradation (ERAD) for misfolded lumenal proteins (PubMed : 15215856, PubMed : 33658201). Forms homotetramers which encircle a large channel traversing the endoplasmic reticulum (ER) membrane (PubMed : 33658201). This allows the retrotranslocation of misfolded proteins from the ER into the cytosol where they are ubiquitinated and degraded by the proteasome (PubMed : 33658201). The channel has a lateral gate within the membrane which provides direct access to membrane proteins with no need to reenter the ER lumen first (PubMed : 33658201). May mediate the interaction between VCP and the misfolded protein (PubMed : 15215856). Also involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation (PubMed : 26565908). By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway (PubMed : 26692333).. (Microbial infection) In case of infection by cytomegaloviruses, it plays a central role in the export from the ER and subsequent degradation of MHC class I heavy chains via its interaction with US11 viral protein, which recognizes and associates with MHC class I heavy chains. Also participates in the degradation process of misfolded cytomegalovirus US2 protein.
See full target information DERL1
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Cancer genomics & proteomics 22:809-823 PubMed40883028

2025

Knockdown of Derlin-1 Represses Cellular Oncogenic Activities in Uveal Melanoma, Enhances its Chemosensitivity to Cisplatin and Reduces Cancer Stem Cell Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Yun Hsieh,Jiun-Rung Chen,Ping-Chung Tsai,Cheng-Yi Chen,An-Chi Liu,Hui-Ying Yeh,Wei-Ting Chen,Chang-Yi Wu,Sung-Chou Li

Nature communications 16:6007 PubMed40593878

2025

Structural insights into the human HRD1 ubiquitin ligase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Liling Guo,Guoyun Liu,Jingjing He,Xiaoxiao Jia,Yonglin He,Zhenhua Wang,Hongwu Qian

Science advances 9:eadi5656 PubMed37831771

2023

The cryo-EM structure of the human ERAD retrotranslocation complex.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Rao,Qian Wang,Deqiang Yao,Ying Xia,Wenguo Li,Yuming Xie,Shaobai Li,Mi Cao,Yafeng Shen,An Qin,Jie Zhao,Yu Cao

Advances in experimental medicine and biology 1415:353-358 PubMed37440056

2023

Inhibition of Ryanodine Receptor 1 Reduces Endoplasmic Reticulum (ER) Stress and Promotes ER Protein Degradation in Cyclic Nucleotide-Gated Channel Deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Hongwei Ma,Rekha Garg,Alfred Lewin,Xi-Qin Ding

Communications biology 6:677 PubMed37380887

2023

SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4 repeat and LRIF1 promoter yet display different modes of action.

Applications

Unspecified application

Species

Unspecified reactive species

Darina Šikrová,Alessandra M Testa,Iris Willemsen,Anita van den Heuvel,Stephen J Tapscott,Lucia Daxinger,Judit Balog,Silvère M van der Maarel

Molecular cell 82:4277-4289.e10 PubMed36283413

2022

ATP13A1 prevents ERAD of folding-competent mislocalized and misoriented proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J McKenna,Benjamin M Adams,Vincent Chu,Joao A Paulo,Sichen Shao

Autophagy 19:805-821 PubMed35920704

2022

TOLLIP-mediated autophagic degradation pathway links the VCP-TMEM63A-DERL1 signaling axis to triple-negative breast cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Tai-Mei Zhang,Li Liao,Shao-Ying Yang,Min-Ying Huang,Yin-Ling Zhang,Ling Deng,Shu-Yuan Hu,Fan Yang,Fang-Lin Zhang,Zhi-Min Shao,Da-Qiang Li

Molecular biology of the cell 33:ar62 PubMed35389766

2022

Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Eli Fritz McDonald,Carleen Mae P Sabusap,Minsoo Kim,Lars Plate

International journal of medical sciences 19:321-330 PubMed35165517

2022

Abnormally decreased renal Klotho is linked to endoplasmic reticulum-associated degradation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

ShaSha Li,JiaWei Kong,LiXia Yu,QiFeng Liu

Nature communications 12:3928 PubMed34168130

2021

Thbs1 induces lethal cardiac atrophy through PERK-ATF4 regulated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Davy Vanhoutte,Tobias G Schips,Alexander Vo,Kelly M Grimes,Tanya A Baldwin,Matthew J Brody,Federica Accornero,Michelle A Sargent,Jeffery D Molkentin
View all publications
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