AB227224

Anti-Desmin antibody

Name: Anti-Desmin antibody (ab227224) | Abcam
Brand: Abcam Limited
SKU: AB227224
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal Desmin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human, Dog, Cat samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DES.

View Alternative Names

Desmin, DES

13 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Paraffin-embedded NCI-H1299 (human lung carcinoma cell line) xenograft tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Immunohistochemical analysis of paraffin-embedded rat muscle tissue stained for Desmin protein at cytoplasm with ab227224 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Immunohistochemical analysis of paraffin-embedded mouse heart tissue stained for Desmin protein at cytoplasm with ab227224 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Paraffin-embedded mouse skeletal muscle tissue stained for Desmin with ab227224 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Desmin antibody (AB227224)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/2000 dilution

All lanes:

RD Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

Supplier Data

Western blot - Anti-Desmin antibody (AB227224)

10% SDS-PAGE

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/3000 dilution

Lane 1:

A549 (human lung carcinoma cell line) whole cell lysate at 30 µg

Lane 2:

HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 54 kDa

true

Unknown

Western blot - Anti-Desmin antibody (AB227224)

10% SDS-PAGE

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/500 dilution

All lanes:

RD (human rhabdomyosarcoma cell line) whole cell lysate at 30 µg

Predicted band size: 54 kDa

true

Supplier Data

Western blot - Anti-Desmin antibody (AB227224)

10% SDS-PAGE

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/1000 dilution

All lanes:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Predicted band size: 54 kDa

true

Supplier Data

Western blot - Anti-Desmin antibody (AB227224)

10% SDS-PAGE

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/1000 dilution

All lanes:

Mouse muscle tissue lysate at 50 µg

Predicted band size: 54 kDa

true

Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody (AB227224)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody (AB227224)

Immunofluorescence analysis of MDCK cells fixed in 4% paraformaldehyde at RT for 15 min labelling Desmin protein at cytoplasm using ab227224 at a 1/500 dilution (Green).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Immunohistochemical analysis of paraffin-embedded dog muscle tissue stained for Desmin protein at cell membrane and cytoplasm with ab227224 at a 1/500 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

Supplier Data

Western blot - Anti-Desmin antibody (AB227224)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/500 dilution

All lanes:

MDCK Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

Supplier Data

Western blot - Anti-Desmin antibody (AB227224)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Desmin antibody (ab227224) at 1/500 dilution

All lanes:

PG-4 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Dog, Cat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DES. The exact immunogen used to generate this antibody is proprietary information.

P17661

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.

Biological function summary

Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.

Pathways

Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.

Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed : 25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed : 24200904, PubMed : 25394388, PubMed : 26724190). May act as a sarcomeric microtubule-anchoring protein : specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

See full target information DES

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 25:64 PubMed38395806

2024

Assessing the effects of tempol on renal fibrosis, inflammation, and oxidative stress in a high-salt diet combined with 5/6 nephrectomy rat model: utilizing oxidized albumin as a biomarker.

Applications

Unspecified application

Species

Unspecified reactive species

Beibei Liu,Yanling Hu,Danyang Tian,Jianlong Dong,Bing-Feng Li

In vivo (Athens, Greece) 37:2421-2432 PubMed37905617

2023

Chemical Carcinogen (Dimethyl-benzanthracene) Induced Transplantable Cancer in Fanconi Anemia (Fanca-/-) Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michael W Epperly,Amitava Mukherjee,Renee Fisher,Donna Shields,Wen Hou,Hong Wang,Lora H Rigatti,Anthony Green,M Saiful Huq,Joel S Greenberger

Cell biology international 47:1126-1135 PubMed36841942

2023

TRIM29 promotes podocyte pyroptosis in diabetic nephropathy through the NF-kB/NLRP3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xu,Zihan Qin,Ce Zhang,Xia Mi,Chi Zhang,Feihong Zhou,Junsheng Wang,Liexiang Zhang,Fei Hua

Journal of gastroenterology and hepatology 38:441-450 PubMed36652457

2023

Antagonizing EZH2 combined with vitamin D3 exerts a synergistic role in anti-fibrosis through bidirectional effects on hepatocytes and hepatic stellate cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghui Zhang,Rongrong Jia,Minjie Chen,Jianjun Wang,Feng Huang,Min Shi,Huiming Sheng,Ling Xu

International journal of molecular medicine 48: PubMed34651657

2021

MALAT1 promotes liver fibrosis by sponging miR‑181a and activating TLR4‑NF‑κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Wang,Qiuju Mou,Zixin Zhu,Luqiang Zhao,Lili Zhu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Desmin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Immunocytochemistry/ Immunofluorescence - Anti-Desmin antibody (AB227224)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Western blot - Anti-Desmin antibody (AB227224)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise