AB8592

Anti-Desmin antibody

Name: Anti-Desmin antibody (ab8592) | Abcam
Brand: Abcam Limited
SKU: AB8592
Price: 640 USD
Availability: InStock
Rating: 4 (16 reviews)

(16 Reviews)

(109 Publications)

Rabbit Polyclonal Desmin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 109 publications. Immunogen corresponding to Full Length Protein corresponding to Human DES.

View Alternative Names

Desmin, DES

1 Images

Lab

Western blot - Anti-Desmin antibody (AB8592)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Desmin antibody (ab8592) at 1/1000 dilution

Lane 1:

RMS13 cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human heart tissue lysate at 20 µg

Lane 5:

C2C12 cell lysate at 20 µg

Lane 6:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 7:

Mouse heart tissue lysate at 20 µg

Lane 8:

L6 cell lysate at 20 µg

Lane 9:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 53 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Full Length Protein corresponding to Human DES. The exact immunogen used to generate this antibody is proprietary information.

P17661

Specificity

In immunoblots, this antibody reacts with a 53 kD polypeptide. It does not react with vimentin or human cytokeratins. This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "See Abreview." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "See Abreview." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "See Abreview." } } }

style

left-column

Product details

Prolonged fixation in buffered formalin can destroy the epitope.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.

Biological function summary

Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.

Pathways

Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.

Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed : 25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed : 24200904, PubMed : 25394388, PubMed : 26724190). May act as a sarcomeric microtubule-anchoring protein : specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

See full target information DES

style

left-column

Publications (109)

Recent publications for all applications. Explore the full list and refine your search

Journal of functional biomaterials 16: PubMed40422839

2025

Wharton's Jelly Bioscaffolds Improve Cardiac Repair with Bone Marrow Mononuclear Stem Cells in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Luize Kremer Gamba,Laiza Kremer Gamba,Camila da Costa,Aline Luri Takejima,Rossana Baggio Simeoni,Isabella Cristina Mendes Rossa,Anna Clara Faidiga Silva,Julia Letícia de Bortolo,Marcos Antônio Denk,Seigo Nagashima,Carlos de Almeida Barbosa,Paulo Cesar Lock Silveira,Júlio César Francisco,Luiz César Guarita-Souza

Nature communications 16:4477 PubMed40368879

2025

DG9 boosts PMO nuclear uptake and exon skipping to restore dystrophic muscle and cardiac function.

Applications

Unspecified application

Species

Unspecified reactive species

Md Nur Ahad Shah,Harry Wilton-Clark,Farhia Haque,Brooklynn Powell,Laura Edellein Sutanto,Radha Maradiya,Pavel Zhabyeyev,Rohini Roy Roshmi,Saeed Anwar,Tejal Aslesh,Kenji Rowel Q Lim,Rika Maruyama,Anne Bigot,Courtney S Young,Scott Bittner,Melissa J Spencer,Hong M Moulton,Gavin Y Oudit,Toshifumi Yokota

GeroScience 46:2033-2049 PubMed37801203

2023

Skeletal muscle myostatin mRNA expression is upregulated in aged human adults with excess adiposity but is not associated with insulin resistance and ageing.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew Wilhelmsen,Francis B Stephens,Andrew J Bennett,Leonidas G Karagounis,Simon W Jones,Kostas Tsintzas

Advanced biology 7:e2300157 PubMed37434585

2023

Tetraspanin CD82 Associates with Trafficking Vesicle in Muscle Cells and Binds to Dysferlin and Myoferlin.

Applications

Unspecified application

Species

Unspecified reactive species

Tatiana Fontelonga,Arielle J Hall,Jaedon L Brown,Youngsook L Jung,Matthew S Alexander,Janice A Dominov,Vincent Mouly,Natassia Vieira,Mayana Zatz,Mariz Vainzof,Emanuela Gussoni

Cells 12: PubMed37048098

2023

Autophagy Inhibitor Chloroquine Downmodulates Hepatic Stellate Cell Activation and Liver Damage in Bile-Duct-Ligated Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Trinh Van Le,Hong-Thuy Phan-Thi,My-Xuan Huynh-Thi,Thanh Minh Dang,Ai Xuan Le Holterman,Gabriele Grassi,Thao-Uyen Nguyen-Luu,Nhung Hai Truong

eLife 12: PubMed36927816

2023

Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Giuseppina Mastrototaro,Pierluigi Carullo,Jianlin Zhang,Beatrice Scellini,Nicoletta Piroddi,Simona Nemska,Maria Carmela Filomena,Simone Serio,Carol A Otey,Chiara Tesi,Fabian Emrich,Wolfgang A Linke,Corrado Poggesi,Simona Boncompagni,Marie-Louise Bang

International journal of molecular sciences 24: PubMed36835551

2023

Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Sergey A Tyganov,Svetlana P Belova,Olga V Turtikova,Ivan M Vikhlyantsev,Tatiana L Nemirovskaya,Boris S Shenkman

EMBO reports 23:e55175 PubMed36278411

2022

The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Dina Aweida,Shenhav Cohen

Methods in molecular biology (Clifton, N.J.) 2566:113-132 PubMed36152246

2022

Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Marie E Esper,Kasun Kodippili,Michael A Rudnicki

Pflugers Archiv : European journal of physiology 474:1171-1183 PubMed35931829

2022

Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle.

Applications

Unspecified application

Species

Unspecified reactive species

I Y Melnikov,Sergey A Tyganov,K A Sharlo,A D Ulanova,I M Vikhlyantsev,T M Mirzoev,B S Shenkman

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmin antibody

Western blot - Anti-Desmin antibody (AB8592)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (109)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise