Rabbit Polyclonal Desmin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 103 publications. Immunogen corresponding to Full Length Protein corresponding to Human DES.
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- GeroScience 46:2033-20492023Skeletal muscle myostatin mRNA expression is upregulated in aged human adults with excess adiposity but is not associated with insulin resistance and ageing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrew Wilhelmsen,Francis B Stephens,Andrew J Bennett,Leonidas G Karagounis,Simon W Jones,Kostas TsintzasPubMed 37801203
- Advanced biology 7:e23001572023Tetraspanin CD82 Associates with Trafficking Vesicle in Muscle Cells and Binds to Dysferlin and Myoferlin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatiana Fontelonga,Arielle J Hall,Jaedon L Brown,Youngsook L Jung,Matthew S Alexander,Janice A Dominov,Vincent Mouly,Natassia Vieira,Mayana Zatz,Mariz Vainzof,Emanuela GussoniPubMed 37434585
- eLife 12:2023Ablation of palladin in adult heart causes dilated cardiomyopathy associated with intercalated disc abnormalities.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiuseppina Mastrototaro,Pierluigi Carullo,Jianlin Zhang,Beatrice Scellini,Nicoletta Piroddi,Simona Nemska,Maria Carmela Filomena,Simone Serio,Carol A Otey,Chiara Tesi,Fabian Emrich,Wolfgang A Linke,Corrado Poggesi,Simona Boncompagni,Marie-Louise BangPubMed 36927816
- International journal of molecular sciences 24:2023Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergey A Tyganov,Svetlana P Belova,Olga V Turtikova,Ivan M Vikhlyantsev,Tatiana L Nemirovskaya,Boris S ShenkmanPubMed 36835551
- EMBO reports 23:e551752022The AAA-ATPase ATAD1 and its partners promote degradation of desmin intermediate filaments in muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDina Aweida,Shenhav CohenPubMed 36278411
- Methods in molecular biology (Clifton, N.J.) 2566:113-1322022Immunofluorescence Labeling of Skeletal Muscle in Development, Regeneration, and Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarie E Esper,Kasun Kodippili,Michael A RudnickiPubMed 36152246
- Pflugers Archiv : European journal of physiology 474:1171-11832022Calpain-dependent degradation of cytoskeletal proteins as a key mechanism for a reduction in intrinsic passive stiffness of unloaded rat postural muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesI Y Melnikov,Sergey A Tyganov,K A Sharlo,A D Ulanova,I M Vikhlyantsev,T M Mirzoev,B S ShenkmanPubMed 35931829
- Journal of functional biomaterials 13:2022Autologous Bone Marrow Mononuclear Cells (BMMC)-Associated Anti-Inflammatory Nanoparticles for Cardiac Repair after Myocardial Infarction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaercio Uemura,Rossana Baggio Simeoni,Paulo André Bispo Machado Júnior,Gustavo Gavazzoni Blume,Luize Kremer Gamba,Murilo Sgarbossa Tonial,Paulo Ricardo Baggio Simeoni,Victoria Stadler Tasca Ribeiro,Rodrigo Silvestre,Katherine Athayde Teixeira de Carvalho,Marcelo Henrique Napimoga,Júlio Cesar Francisco,Luiz Cesar Guarita-SouzaPubMed 35645267
- Renal failure 44:694-7052022Vitamin D/vitamin D receptor/Atg16L1 axis maintains podocyte autophagy and survival in diabetic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLang Shi,Chao Xiao,Yafei Zhang,Yao Xia,Hongchu Zha,Jiefu Zhu,Zhixia SongPubMed 35469547
- International journal of molecular sciences 22:2021Natural History of a Mouse Model Overexpressing the Dp71 Dystrophin Isoform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenji Rowel Q Lim,Md Nur Ahad Shah,Stanley Woo,Harry Wilton-Clark,Pavel Zhabyeyev,Faqi Wang,Rika Maruyama,Gavin Y Oudit,Toshifumi YokotaPubMed 34884423
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, 0.01% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Full Length Protein corresponding to Human DES. The exact immunogen used to generate this antibody is proprietary information. Database link P17661
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
| Rat | Tested | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes See Abreview. |
Species Rat | Dilution info - | Notes See Abreview. |
Species Human | Dilution info - | Notes See Abreview. |
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Target data
Function
Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed:25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed:24200904, PubMed:25394388, PubMed:26724190). May act as a sarcomeric microtubule-anchoring protein: specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).
Alternative names
Desmin, DES
Recommended products
Rabbit Polyclonal Desmin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 103 publications. Immunogen corresponding to Full Length Protein corresponding to Human DES.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 0.2% BSA, 0.01% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Full Length Protein corresponding to Human DES. The exact immunogen used to generate this antibody is proprietary information. Database link P17661
- Purification technique
- Affinity purification Protein G
- Specificity
In immunoblots, this antibody reacts with a 53 kD polypeptide.
It does not react with vimentin or human cytokeratins.
This antibody can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas, and carcinomas.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Prolonged fixation in buffered formalin can destroy the epitope.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.
- Biological function summary
Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.
- Pathways
Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.
- Associated diseases and disorders
Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Desmin antibody (ab8592)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Desmin antibody (ab8592) at 1/1000 dilution
Lane 1: RMS13 cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: Human skeletal muscle tissue lysate at 20 µg
Lane 4: Human heart tissue lysate at 20 µg
Lane 5: C2C12 cell lysate at 20 µg
Lane 6: Mouse skeletal muscle tissue lysate at 20 µg
Lane 7: Mouse heart tissue lysate at 20 µg
Lane 8: L6 cell lysate at 20 µg
Lane 9: Rat heart tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 53 kDa
Exposure time: 3min
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