AB6322

Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

Name: Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (ab6322) | Abcam
Brand: Abcam Limited
SKU: AB6322
Price: 400 USD
Availability: InStock
Rating: 4 (6 reviews)

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What is this?

(6 Reviews)

(63 Publications)

Mouse Monoclonal Desmin antibody. Cytoskeleton marker. Suitable for IHC-P and reacts with Mouse, Rat, Human samples. Cited in 63 publications.

View Alternative Names

Desmin, DES

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

IHC image of Desmin staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6322, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

Image shows cytomplasmic staining on smooth muscle of rat colon.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Desmin with ab6322 at a dulution of 1/200. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). A ready to use HRP-conjugated goat anti-rabbit IgG H&L was used as the secondary antibody. Counter stained with Hematoxylin.

Image shows cytomplasmic staining on mouse cardiac muscle.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

DE-U-10

Isotype

IgG1

Light chain type

unknown

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Goat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Lizard": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Snake": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.

Biological function summary

Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.

Pathways

Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.

Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed : 25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed : 24200904, PubMed : 25394388, PubMed : 26724190). May act as a sarcomeric microtubule-anchoring protein : specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

See full target information DES

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Publications (63)

Recent publications for all applications. Explore the full list and refine your search

American journal of physiology. Gastrointestinal and liver physiology 328:G136-G144 PubMed39745592

2025

Local tissue response to a C-X-C motif chemokine ligand 12 therapy for fecal incontinence in a rabbit model.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Ruetten,Shannon S Lankford,Abolfazl S Abdolmaleki,Nicholas Edenhoffer,Gopal Badlani,James K Williams

Cell biology and toxicology 41:5 PubMed39707064

2024

Investigating the role of Wnt3a and Wnt5a as critical factors of hepatic stellate cell activation in acute toxicant-induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren N Rutt,David J Orlicky,Rebecca L McCullough

Cells 13: PubMed39404414

2024

Kisspeptin Alleviates Human Hepatic Fibrogenesis by Inhibiting TGFβ Signaling in Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kavita Prasad,Dipankar Bhattacharya,Shams Gamal Eldin Shams,Kimberly Izarraras,Tia Hart,Brent Mayfield,Maryjka B Blaszczyk,Zhongren Zhou,Utpal B Pajvani,Scott L Friedman,Moshmi Bhattacharya

Glia 73:140-158 PubMed39392208

2024

The role of oligodendrocyte progenitor cells in the spatiotemporal vascularization of the human and mouse neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyi Liu,Zhiruo Kang,Min Yang,Fangbing Chen,Mingyang Xia,Wenjuan Dai,Shiyi Zheng,Huiyao Chen,Q Richard Lu,Wenhao Zhou,Yifeng Lin

EMBO reports 25:1055-1074 PubMed38351372

2024

XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Renjie Hong,Yanjie Tan,Xiaoyu Tian,Zhenzhou Huang,Jiaying Wang,Hua Ni,Jia Yang,Weiwen Bu,Song Yang,Te Li,Fan Yu,Weilong Zhong,Tao Sun,Xiaohong Wang,Dengwen Li,Min Liu,Yunfan Yang,Jun Zhou

Cell reports 42:112051 PubMed36729831

2023

Spatial compartmentalization of signaling imparts source-specific functions on secreted factors.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Groppa,Paolo Martini,Nima Derakhshan,Marine Theret,Morten Ritso,Lin Wei Tung,Yu Xin Wang,Hesham Soliman,Mark Stephen Hamer,Laura Stankiewicz,Christine Eisner,Le Nevé Erwan,Chihkai Chang,Lin Yi,Jack H Yuan,Sunny Kong,Curtis Weng,Josephine Adams,Lucas Chang,Anne Peng,Helen M Blau,Chiara Romualdi,Fabio M V Rossi

International journal of molecular sciences 23: PubMed36430504

2022

Immunohistochemical Changes in the Testicular Excurrent Duct System of Healthy, Male Japanese Quail () Observed at 4, 6-7, 12, and 52 Weeks of Age.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed I A Ibrahim,June H Williams,Christo J Botha

iScience 25:104912 PubMed36060050

2022

Lung fibrogenic microenvironment in mouse reconstitutes human alveolar structure and lung tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Miyata,Koichi Hasegawa,Toshi Menju,Akihiko Yoshizawa,Akira Watanabe,Toyohiro Hirai,Hiroshi Date,Atsuyasu Sato

Cell stem cell 29:1246-1261.e6 PubMed35931033

2022

Lymphatics and fibroblasts support intestinal stem cells in homeostasis and injury.

Applications

Unspecified application

Species

Unspecified reactive species

Norihiro Goto,Saori Goto,Shinya Imada,Sahar Hosseini,Vikram Deshpande,Ömer H Yilmaz

Scientific reports 12:9831 PubMed35701470

2022

Global O-GlcNAcylation changes impact desmin phosphorylation and its partition toward cytoskeleton in C2C12 skeletal muscle cells differentiated into myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Claeyssen,Bruno Bastide,Caroline Cieniewski-Bernard

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [DE-U-10] - Cytoskeleton Marker (AB6322)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (63)

Product promise