AB95967

Anti-Desmocollin 2 antibody

Name: Anti-Desmocollin 2 antibody (ab95967) | Abcam
Brand: Abcam Limited
SKU: AB95967
Price: 450 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal Desmocollin 2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DSC2 aa 450 to C-terminus.

View Alternative Names

CDHF2, DSC3, DSC2, Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

Paraffin embedded OVCAR3 xenograft tissue stained for Desmocollin 2 using ab95967 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Desmocollin 2 antibody (AB95967)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Desmocollin 2 antibody (ab95967) at 1/500 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DSC2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02487

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 2 also known as DSC2 is a cadherin protein and an important component of desmosomes which are structures that facilitate cell-cell adhesion in epithelial tissues. It has a molecular weight of approximately 100 kDa. This protein expresses strongly in tissues rich in desmosomes such as the epidermis and cardiac tissue. Desmocollin 2 stabilizes cells against shearing forces ensuring that they adhere tightly by interacting with other desmosomal proteins.

Biological function summary

Desmocollin 2 plays a role in maintaining tissue integrity and cohesion. It participates as a part of the desmosomal complex where it interacts with other cadherin family members like desmogleins and anchors to the intermediate filaments inside the cell. These interactions help to maintain the architecture and structural support during mechanical stress especially in tissues undergoing constant stress like the heart and skin.

Pathways

Desmocollin 2's mechanical role intersects with various cell signaling pathways. Notably it plays a role in the Wnt signaling pathway which is important for cell proliferation and differentiation. The protein also interacts with plakoglobin a protein involved in beta-catenin regulation within these pathways. Desmocollin 2's involvement in cell adhesion and signaling nodes suggests its contribution to further complex signaling cascades.

Desmocollin 2 mutations and disruptions associate with arrhythmogenic right ventricular cardiomyopathy (ARVC) and certain skin blistering conditions. In ARVC the weakened cell adhesion due to desmocollin 2 defects can lead to fibrofatty replacement of myocardium impacting cardiac function. The protein plakoglobin often appears as a related factor in these diseases as both proteins interact at the desmosomal junctions and contribute to cell adhesion integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 33596089). Promotes timely incorporation of DSG2 into desmosome intercellular junctions and promotes interaction of desmosome cell junctions with intermediate filament cytokeratin, via modulation of DSP phosphorylation (PubMed : 33596089). Plays an important role in desmosome-mediated maintenance of intestinal epithelial cell intercellular adhesion strength and barrier function (PubMed : 33596089). Positively regulates wound healing of intestinal mucosa via promotion of epithelial cell migration, and also plays a role in mechanotransduction of force between intestinal epithelial cells and extracellular matrix (PubMed : 31967937). May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. May promote p38MAPK signaling activation that facilitates keratinocyte migration (By similarity).

See full target information DSC2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology 27: PubMed40879390

2025

Comprehensive analysis of desmosomal protein remodelling identifies desmocollin-2 as potential biomarker for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Zhenliang Hu,Lingmin Wu,Deniz Akdis,Weiquan Ye,Ardan M Saguner,Mingming Su,Hongbin Dong,Zhongli Chen,Dan Hu,Shengshou Hu,Firat Duru,Liang Chen

Journal of Cancer 14:2619-2632 PubMed37779876

2023

Validation the role of desmocollin-2 in osteosarcoma based on single cell and bulk RNA seq and experimental analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxing Zeng,Yu Sun,Yunan Man,Haijun Tang,Long Xie,Maolin He

International journal of molecular sciences 24: PubMed37446408

2023

EPs 7630 Stimulates Tissue Repair Mechanisms and Modifies Tight Junction Protein Expression in Human Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fang,Liang Zhou,Žarko Kulić,Martin D Lehner,Michael Tamm,Michael Roth

Frontiers in nutrition 7:628405 PubMed33521042

2021

LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Paul A Roberson,C Brooks Mobley,Matthew A Romero,Cody T Haun,Shelby C Osburn,Petey W Mumford,Christopher G Vann,Rory A Greer,Arny A Ferrando,Michael D Roberts

Experimental and toxicologic pathology : official 66:437-44 PubMed25153024

2014

Immunohistological characterization of intercellular junction proteins in rhesus macaque intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjeev Gumber,Asma Nusrat,Francois Villinger

The Journal of biological chemistry 286:1499-507 PubMed21071449

2010

Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes.

Applications

Species

Dog

Natasa Resnik,Kristina Sepcic,Ana Plemenitas,Reinhard Windoffer,Rudolf Leube,Peter Veranic

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmocollin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

Western blot - Anti-Desmocollin 2 antibody (AB95967)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise