AB126768

Anti-Desmocollin 3 antibody [EPR7486]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal Desmocollin 3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

CDHF3, DSC4, DSC3, Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP

4 Images

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

ab126768 at 1/10 dilution staining Desmocollin 3 in permeabilized A431 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).

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Western blot - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

All lanes:

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/10000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

Human skin lysate at 10 µg

Lane 3:

Human espophagus lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 100 kDa

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Lab

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (ab126768) at 1/200000 dilution

Lane 1:

Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate

Lane 2:

293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate

Lane 3:

293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate

Lane 4:

293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 100 kDa

Observed band size: 100 kDa

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Exposure time: 40s

OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7486

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cross-reacts weakly with Desmocollin 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 3 also known as DSC3 is a desmosomal cadherin protein with a molecular mass of approximately 100 kDa. It plays an important role in cell adhesion by forming part of the desmosomes which are specialized junctions that connect adjacent cells. DSC3 is primarily expressed in epithelial tissues such as the skin and mucous membranes. By contributing to the structural integrity of epithelial cell layers DSC3 ensures cells stick together firmly providing mechanical stability.

Biological function summary

Desmocollin 3 is a part of the cadherin superfamily which are calcium-dependent adhesion proteins. Within desmosomes DSC3 interacts closely with desmogleins and other cadherins to form strong intercellular connections. This protein is an essential component of the desmosomal adhesion complex that helps maintain tissue architecture. The interaction between DSC3 and other cadherins regulates the cohesion and communication between epithelial cells which is vital for normal tissue function.

Pathways

Desmocollin 3 is an important participant in the cadherin signaling pathway and intercellular junction organization. These pathways are important for the regulation of tissue morphogenesis and the maintenance of cell polarity and tissue architecture. DSC3 functions alongside proteins like plakoglobin an integral part of the desmosome complex that helps bridge cadherins with cytoskeletal elements. This intricate network supports tissue resilience and adaptive responses to mechanical stress.

Desmocollin 3 has strong associations with skin diseases such as pemphigus vulgaris and squamous cell carcinoma. In pemphigus vulgaris antibodies target desmosomal components like DSC3 leading to a loss of cell adhesion and blistering of the skin. In squamous cell carcinoma aberrant expression or function of DSC3 can contribute to the invasive potential of cancer cells. DSC3's interaction with desmogleins in these conditions highlights its importance in disease mechanisms and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (By similarity). Required for cell-cell adhesion in the epidermis, as a result required for the maintenance of the dermal cohesion and the dermal barrier function (PubMed : 19717567). Required for cell-cell adhesion of epithelial cell layers surrounding the telogen hair club, as a result plays an important role in telogen hair shaft anchorage (By similarity). Essential for successful completion of embryo compaction and embryo development (By similarity).

See full target information DSC3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Pulmonary pharmacology & therapeutics 63:101931 PubMed32818548

2020

The proliferative and anti-apoptosis functions of KGF/KGFR contributes to bronchial epithelial repair in asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiongbo Wang,Hongju Sun,Yunshun Liu

Turkish journal of medical sciences 46:1854-1861 PubMed28081338

2016

New immunohistochemical markers in the differential diagnosisof nonsmall cell lung carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ferda Bir,Duygu Çeliker,Binnaz Fatma Evyapan,Arzu Yaren,Tamer Edirne

Metallomics : integrated biometal science 5:1357-67 PubMed23963610

2013

Sodium metavanadate exhibits carcinogenic tendencies in vitro in immortalized human bronchial epithelial cells.

Applications

Species

Human

Lisa Passantino,Alexandra B Muñoz,Max Costa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmocollin 3 antibody [EPR7486]

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] (AB126768)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise