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Rabbit Recombinant Monoclonal Desmocollin 3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176), expandable thumbnail
  • Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176), expandable thumbnail
  • OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Not recommended
Tested
Mouse
Not recommended
Predicted
Not recommended
Predicted
Rat
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms.

Alternative names

CDHF3, DSC4, DSC3, Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP

Recommended products

Rabbit Recombinant Monoclonal Desmocollin 3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7486
Purification technique
Affinity purification Protein A
Specificity

This antibody cross-reacts weakly with Desmocollin 2.

Dissociation constant
2.4 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248176 is the carrier-free version of Anti-Desmocollin 3 antibody [EPR7486] ab126768.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Desmocollin 3 also known as DSC3 is a desmosomal cadherin protein with a molecular mass of approximately 100 kDa. It plays an important role in cell adhesion by forming part of the desmosomes which are specialized junctions that connect adjacent cells. DSC3 is primarily expressed in epithelial tissues such as the skin and mucous membranes. By contributing to the structural integrity of epithelial cell layers DSC3 ensures cells stick together firmly providing mechanical stability.

Biological function summary

Desmocollin 3 is a part of the cadherin superfamily which are calcium-dependent adhesion proteins. Within desmosomes DSC3 interacts closely with desmogleins and other cadherins to form strong intercellular connections. This protein is an essential component of the desmosomal adhesion complex that helps maintain tissue architecture. The interaction between DSC3 and other cadherins regulates the cohesion and communication between epithelial cells which is vital for normal tissue function.

Pathways

Desmocollin 3 is an important participant in the cadherin signaling pathway and intercellular junction organization. These pathways are important for the regulation of tissue morphogenesis and the maintenance of cell polarity and tissue architecture. DSC3 functions alongside proteins like plakoglobin an integral part of the desmosome complex that helps bridge cadherins with cytoskeletal elements. This intricate network supports tissue resilience and adaptive responses to mechanical stress.

Associated diseases and disorders

Desmocollin 3 has strong associations with skin diseases such as pemphigus vulgaris and squamous cell carcinoma. In pemphigus vulgaris antibodies target desmosomal components like DSC3 leading to a loss of cell adhesion and blistering of the skin. In squamous cell carcinoma aberrant expression or function of DSC3 can contribute to the invasive potential of cancer cells. DSC3's interaction with desmogleins in these conditions highlights its importance in disease mechanisms and potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176), expandable thumbnail

    Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176)

    This data was developed using Anti-Desmocollin 3 antibody [EPR7486] ab126768, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Desmocollin 3 antibody [EPR7486] (Anti-Desmocollin 3 antibody [EPR7486] ab126768) at 1/200000 dilution

    Lane 1: Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate

    Lane 2: 293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate

    Lane 3: 293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate

    Lane 4: 293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa

    Observed band size: 100 kDa

    Exposure time: 40s

  • Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176)

    This data was developed using Anti-Desmocollin 3 antibody [EPR7486] ab126768, the same antibody clone in a different buffer formulation.

    Anti-Desmocollin 3 antibody [EPR7486] ab126768 at 1/10 dilution staining Desmocollin 3 in permeabilized A431 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).

  • Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176), expandable thumbnail

    Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176)

    This data was developed using Anti-Desmocollin 3 antibody [EPR7486] ab126768, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Desmocollin 3 antibody [EPR7486] (Anti-Desmocollin 3 antibody [EPR7486] ab126768) at 1/10000 dilution

    Lane 1: A431 cell lysate at 10 µg

    Lane 2: Human skin lysate at 10 µg

    Lane 3: Human espophagus lysate at 10 µg

    Secondary

    All lanes: Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 100 kDa

  • OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176), expandable thumbnail

    OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (ab248176)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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