AB248176

Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Desmocollin 3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CDHF3, DSC4, DSC3, Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP

4 Images

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

This data was developed using ab126768, the same antibody clone in a different buffer formulation.

ab126768 at 1/10 dilution staining Desmocollin 3 in permeabilized A431 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).

Unknown

Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

This data was developed using ab126768, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (<a href='/en-us/products/primary-antibodies/desmocollin-3-antibody-epr7486-ab126768'>ab126768</a>) at 1/10000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

Human skin lysate at 10 µg

Lane 3:

Human espophagus lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 100 kDa

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Lab

Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

This data was developed using ab126768, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Desmocollin 3 antibody [EPR7486] (<a href='/en-us/products/primary-antibodies/desmocollin-3-antibody-epr7486-ab126768'>ab126768</a>) at 1/200000 dilution

Lane 1:

Empty vector transfected 293T (Human embryonic kidney epithelial cell) whole cell lysate

Lane 2:

293T cells transfected with myc-his tagged full length Desmocollin-1 expression vector, whole cell lysate

Lane 3:

293T cells transfected with myc-his tagged full length Desmocollin-2 expression vector, whole cell lysate

Lane 4:

293T cells transfected with myc-his tagged full length Desmocollin-3 expression vector, whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 100 kDa

Observed band size: 100 kDa

false

Exposure time: 40s

OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-Desmocollin 3 antibody [EPR7486]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7486

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody cross-reacts weakly with Desmocollin 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab248176 is the carrier-free version of ab126768.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 3 also known as DSC3 is a desmosomal cadherin protein with a molecular mass of approximately 100 kDa. It plays an important role in cell adhesion by forming part of the desmosomes which are specialized junctions that connect adjacent cells. DSC3 is primarily expressed in epithelial tissues such as the skin and mucous membranes. By contributing to the structural integrity of epithelial cell layers DSC3 ensures cells stick together firmly providing mechanical stability.

Biological function summary

Desmocollin 3 is a part of the cadherin superfamily which are calcium-dependent adhesion proteins. Within desmosomes DSC3 interacts closely with desmogleins and other cadherins to form strong intercellular connections. This protein is an essential component of the desmosomal adhesion complex that helps maintain tissue architecture. The interaction between DSC3 and other cadherins regulates the cohesion and communication between epithelial cells which is vital for normal tissue function.

Pathways

Desmocollin 3 is an important participant in the cadherin signaling pathway and intercellular junction organization. These pathways are important for the regulation of tissue morphogenesis and the maintenance of cell polarity and tissue architecture. DSC3 functions alongside proteins like plakoglobin an integral part of the desmosome complex that helps bridge cadherins with cytoskeletal elements. This intricate network supports tissue resilience and adaptive responses to mechanical stress.

Desmocollin 3 has strong associations with skin diseases such as pemphigus vulgaris and squamous cell carcinoma. In pemphigus vulgaris antibodies target desmosomal components like DSC3 leading to a loss of cell adhesion and blistering of the skin. In squamous cell carcinoma aberrant expression or function of DSC3 can contribute to the invasive potential of cancer cells. DSC3's interaction with desmogleins in these conditions highlights its importance in disease mechanisms and potential as a therapeutic target.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (By similarity). Required for cell-cell adhesion in the epidermis, as a result required for the maintenance of the dermal cohesion and the dermal barrier function (PubMed : 19717567). Required for cell-cell adhesion of epithelial cell layers surrounding the telogen hair club, as a result plays an important role in telogen hair shaft anchorage (By similarity). Essential for successful completion of embryo compaction and embryo development (By similarity).

See full target information DSC3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1025 PubMed39164573

2024

CD58 defines regulatory macrophages within the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Wu,Xiaoni Zhan,Meixi Jiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

Western blot - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

OI-RD Scanning - Anti-Desmocollin 3 antibody [EPR7486] - BSA and Azide free (AB248176)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise