Mouse Monoclonal Desmoglein 1/DSG1 antibody. Carrier free. Suitable for IHC-Fr and reacts with Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (AB269569), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-1-dsg1-antibody-27b2-bsa-and-azide-free-ab269569--immunohistochemistry-frozen-sections-img1184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (AB269569)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-Fr | |
|---|---|
| Human | Tested |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Alternative names
CDHF4, DSG1, Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DG1, DGI
Recommended products
Mouse Monoclonal Desmoglein 1/DSG1 antibody. Carrier free. Suitable for IHC-Fr and reacts with Human samples.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 27B2
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab269569 is the carrier-free version of Anti-Desmoglein 1/DSG1 antibody [27B2] ab12077.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmoglein 1 often abbreviated as DSG1 or Desmoglein-1 is a protein that plays a significant mechanical role in cell adhesion. This protein with a molecular mass of approximately 122 kDa belongs to the cadherin superfamily. It is mainly expressed in the epidermis specifically in the outermost layers of the skin. Desmoglein 1 is an important component of desmosomes which are specialized cell structures responsible for maintaining the integrity of epithelial tissues.
- Biological function summary
Desmogleins including Desmoglein 1 facilitate adhesion between keratinocytes the predominant cell type in the epidermis. Desmoglein 1 interacts intimately within the desmosome complex contributing to the mechanical stability and cohesion of the cellular layers in the skin. These interactions help form a barrier that protects underlying tissues from environmental assaults and water loss. It is important in the process of epidermal differentiation important for proper skin formation structure and function.
- Pathways
Desmoglein 1 integrates into several signaling and structural pathways related to cell adhesion and epidermal maintenance. Desmoglein 1 is prominently involved in the Wnt signaling pathway which is important for skin development and renewal. This involvement establishes its functional link with other adhesion proteins such as desmocollins and regulatory molecules like plakoglobin which mediate the cytoskeletal association within desmosomes.
- Associated diseases and disorders
Desmoglein 1 has clinical relevance in conditions such as pemphigus foliaceus and skin inflammation disorders. Antibodies targeting DSG1 often referred to as anti-Desmoglein 1 antibodies play a pathogenic role in pemphigus foliaceus disrupting cell adhesion and resulting in blistering. Additionally Desmoglein 1's interaction with other proteins like Desmoglein 3 becomes altered in certain auto-immune diseases disturbing normal skin integrity and leading to disease phenotypes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-1-dsg1-antibody-27b2-bsa-and-azide-free-ab269569--immunohistochemistry-frozen-sections-img1184.jpg "Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)")
Immunohistochemistry (Frozen sections) - Anti-Desmoglein 1/DSG1 antibody [27B2] - BSA and Azide free (ab269569)
IHC image of Anti-Desmoglein 1/DSG1 antibody [27B2] ab12077 staining in 100% methanol fixed frozen tissue section of human skin.
Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with Anti-Desmoglein 1/DSG1 antibody [27B2] ab12077 (1μg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119 (Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647)) and DAPI for 1 hour at room temperature.
The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound Anti-Desmoglein 1/DSG1 antibody [27B2] ab12077.
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antibody concentrations and incubation times.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-Desmoglein 1/DSG1 antibody [27B2] ab12077).
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