AB133662

Anti-Desmoglein 1/DSG1 antibody [EP6765]

RabMAb
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Monoclonal Desmoglein 1/DSG1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CDHF4, DSG1, Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DG1, DGI

3 Images

Unknown

Western blot - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

All lanes:

Western blot - Anti-Desmoglein 1/DSG1 antibody [EP6765] (ab133662) at 1/1000 dilution

All lanes:

Human fetal skin lysate at 10 µg

Secondary

All lanes:

HRP conjugated Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 114 kDa

false

OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

CiteAb

Western blot - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

Desmoglein 1/DSG1 western blot using anti-Desmoglein 1/DSG1 antibody [EP6765] ab133662. Publication image and figure legend from Klymenko, T., Gu, Q., et al., 2017, J Virol, PubMed 29021401.

ab133662 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab133662 please see the product overview.

Western blot analysis of protein levels encoded by selected, significantly altered mRNAs (Table 2). Protein extracts were prepared from undifferentiated (U) and differentiated (D) HPV-negative NIKS and HPV16-positive NIKS16 and W12 cell populations. Much greater levels of involucrin (invol) were detected in the differentiated than in the undifferentiated cell populations, indicating that differentiation was achieved. GAPDH was used as a protein loading control. (A) Protein levels corresponding to significantly upregulated mRNAs; (B) protein levels corresponding to significantly downregulated mRNAs.

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP6765

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 1 often abbreviated as DSG1 or Desmoglein-1 is a protein that plays a significant mechanical role in cell adhesion. This protein with a molecular mass of approximately 122 kDa belongs to the cadherin superfamily. It is mainly expressed in the epidermis specifically in the outermost layers of the skin. Desmoglein 1 is an important component of desmosomes which are specialized cell structures responsible for maintaining the integrity of epithelial tissues.

Biological function summary

Desmogleins including Desmoglein 1 facilitate adhesion between keratinocytes the predominant cell type in the epidermis. Desmoglein 1 interacts intimately within the desmosome complex contributing to the mechanical stability and cohesion of the cellular layers in the skin. These interactions help form a barrier that protects underlying tissues from environmental assaults and water loss. It is important in the process of epidermal differentiation important for proper skin formation structure and function.

Pathways

Desmoglein 1 integrates into several signaling and structural pathways related to cell adhesion and epidermal maintenance. Desmoglein 1 is prominently involved in the Wnt signaling pathway which is important for skin development and renewal. This involvement establishes its functional link with other adhesion proteins such as desmocollins and regulatory molecules like plakoglobin which mediate the cytoskeletal association within desmosomes.

Desmoglein 1 has clinical relevance in conditions such as pemphigus foliaceus and skin inflammation disorders. Antibodies targeting DSG1 often referred to as anti-Desmoglein 1 antibodies play a pathogenic role in pemphigus foliaceus disrupting cell adhesion and resulting in blistering. Additionally Desmoglein 1's interaction with other proteins like Desmoglein 3 becomes altered in certain auto-immune diseases disturbing normal skin integrity and leading to disease phenotypes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

See full target information DSG1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 91: PubMed29021401

2017

RNA-Seq Analysis of Differentiated Keratinocytes Reveals a Massive Response to Late Events during Human Papillomavirus 16 Infection, Including Loss of Epithelial Barrier Function.

Applications

Unspecified application

Species

Unspecified reactive species

T Klymenko,Q Gu,I Herbert,A Stevenson,V Iliev,G Watkins,C Pollock,R Bhatia,K Cuschieri,P Herzyk,D Gatherer,S V Graham

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 1/DSG1 antibody [EP6765]

Western blot - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

OI-RD Scanning - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

Western blot - Anti-Desmoglein 1/DSG1 antibody [EP6765] (AB133662)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise