AB209490

Anti-Desmoglein 1/DSG1 antibody [EPR20382]

BOND RX™ Validated
20ul selling size
Recombinant
Advanced Validation
RabMAb
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(4 Publications)

Rabbit Recombinant Monoclonal Desmoglein 1/DSG1 antibody. Suitable for IHC-P, WB, mIHC and reacts with Human samples. Cited in 4 publications.

View Alternative Names

CDHF4, DSG1, Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DG1, DGI

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Positive staining on human skin (PMID 15606502) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Desmoglein 1/DSG1 with ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use. Membranous staining on human esophagus (PMID : 24220297) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) ready to use.

Heat-mediated antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).

Multiplex immunohistochemistry - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Panel A : merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin. Panel B : anti-TRP2/DCT stained on melanocytes. Panel C : anti-Langerin stained on Langerhans cells. Panel D : anti-Desmoglein 1/DSG1 stained on epidermis. The section was incubated in three rounds of staining : in the order of ab236259, ab283686, and ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Unknown

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

The expression profile and molecular weight observed are consistent with what have been described in the literature (PMID : 10634593, 29192645)

Observed MW : 150kDa

Block/diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (ab209490) at 1/5000 dilution

Lane 1:

Human skin lysates prepared in RIPA lysis method at 15 µg

Lane 2:

Human skin lysates prepared in 1%SDS Hot lysis method at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

Predicted band size: 114 kDa

false

Carrier free

Anti-Desmoglein 1/DSG1 antibody [EPR20382] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20382

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, mIHC, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/1000", "mIHC-species-notes": "Perform heat mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 1 often abbreviated as DSG1 or Desmoglein-1 is a protein that plays a significant mechanical role in cell adhesion. This protein with a molecular mass of approximately 122 kDa belongs to the cadherin superfamily. It is mainly expressed in the epidermis specifically in the outermost layers of the skin. Desmoglein 1 is an important component of desmosomes which are specialized cell structures responsible for maintaining the integrity of epithelial tissues.

Biological function summary

Desmogleins including Desmoglein 1 facilitate adhesion between keratinocytes the predominant cell type in the epidermis. Desmoglein 1 interacts intimately within the desmosome complex contributing to the mechanical stability and cohesion of the cellular layers in the skin. These interactions help form a barrier that protects underlying tissues from environmental assaults and water loss. It is important in the process of epidermal differentiation important for proper skin formation structure and function.

Pathways

Desmoglein 1 integrates into several signaling and structural pathways related to cell adhesion and epidermal maintenance. Desmoglein 1 is prominently involved in the Wnt signaling pathway which is important for skin development and renewal. This involvement establishes its functional link with other adhesion proteins such as desmocollins and regulatory molecules like plakoglobin which mediate the cytoskeletal association within desmosomes.

Desmoglein 1 has clinical relevance in conditions such as pemphigus foliaceus and skin inflammation disorders. Antibodies targeting DSG1 often referred to as anti-Desmoglein 1 antibodies play a pathogenic role in pemphigus foliaceus disrupting cell adhesion and resulting in blistering. Additionally Desmoglein 1's interaction with other proteins like Desmoglein 3 becomes altered in certain auto-immune diseases disturbing normal skin integrity and leading to disease phenotypes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

See full target information DSG1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7260 PubMed40770025

2025

Phase synchrony between prefrontal noradrenergic and cholinergic signals indexes inhibitory control.

Applications

Unspecified application

Species

Unspecified reactive species

Yuxiang Andy Liu,Yuhan Nong,Jiesi Feng,Guochuan Li,Paul Sajda,Yulong Li,Qi Wang

Investigative ophthalmology & visual science 65:43 PubMed39330987

2024

Single-Cell Transcriptomic Analysis Reveals Dynamic Cellular Processes in Corneal Epithelium During Wound Healing in Cynomolgus Monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhou,Zhuo-Xing Shi,Zhong Liu,Shu-Rui Ke,Chao-Yang Wang,Xiao-Lin Liang,Qiu-Ling Hu,Qi-Kai Zhang,Dong-Liang Wang,Li Sun,Yu-Heng Lin,Qi Dai,Ying-Feng Zheng

Mucosal immunology 16:567-577 PubMed37302713

2023

Mast cells disrupt the function of the esophageal epithelial barrier.

Applications

Unspecified application

Species

Unspecified reactive species

Mirelle T A Kleuskens,Marie K Bek,Youmna Al Halabi,Bart R J Blokhuis,Mara A P Diks,Maria L Haasnoot,Johan Garssen,Albert J Bredenoord,Betty C A M van Esch,Frank A Redegeld

Allergy 77:1510-1521 PubMed34458999

2021

Butyrate and propionate restore interleukin 13-compromised esophageal epithelial barrier function.

Applications

Unspecified application

Species

Unspecified reactive species

Mirelle T A Kleuskens,Maria L Haasnoot,Bart M Herpers,Marleen T J van Ampting,Albert J Bredenoord,Johan Garssen,Frank A Redegeld,Betty C A M van Esch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 1/DSG1 antibody [EPR20382]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Multiplex immunohistochemistry - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR20382] (AB209490)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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