Rabbit Recombinant Monoclonal Desmoglein 1/DSG1 antibody. Carrier free. Suitable for IHC-P, WB, mIHC and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IHC-P | WB | mIHC | |
---|---|---|---|
Human | Tested | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol |
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Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
CDHF4, DSG1, CDHF4, Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DG1, DGI
Rabbit Recombinant Monoclonal Desmoglein 1/DSG1 antibody. Carrier free. Suitable for IHC-P, WB, mIHC and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR20382
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab236259 is the carrier-free version of Anti-Desmoglein 1/DSG1 antibody [EPR20382] ab209490.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Desmoglein 1 often abbreviated as DSG1 or Desmoglein-1 is a protein that plays a significant mechanical role in cell adhesion. This protein with a molecular mass of approximately 122 kDa belongs to the cadherin superfamily. It is mainly expressed in the epidermis specifically in the outermost layers of the skin. Desmoglein 1 is an important component of desmosomes which are specialized cell structures responsible for maintaining the integrity of epithelial tissues.
Desmogleins including Desmoglein 1 facilitate adhesion between keratinocytes the predominant cell type in the epidermis. Desmoglein 1 interacts intimately within the desmosome complex contributing to the mechanical stability and cohesion of the cellular layers in the skin. These interactions help form a barrier that protects underlying tissues from environmental assaults and water loss. It is important in the process of epidermal differentiation important for proper skin formation structure and function.
Desmoglein 1 integrates into several signaling and structural pathways related to cell adhesion and epidermal maintenance. Desmoglein 1 is prominently involved in the Wnt signaling pathway which is important for skin development and renewal. This involvement establishes its functional link with other adhesion proteins such as desmocollins and regulatory molecules like plakoglobin which mediate the cytoskeletal association within desmosomes.
Desmoglein 1 has clinical relevance in conditions such as pemphigus foliaceus and skin inflammation disorders. Antibodies targeting DSG1 often referred to as anti-Desmoglein 1 antibodies play a pathogenic role in pemphigus foliaceus disrupting cell adhesion and resulting in blistering. Additionally Desmoglein 1's interaction with other proteins like Desmoglein 3 becomes altered in certain auto-immune diseases disturbing normal skin integrity and leading to disease phenotypes.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Desmoglein 1/DSG1 with Anti-Desmoglein 1/DSG1 antibody [EPR20382] ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Positive staining on human skin (PMID 15606502) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use.
Heat-mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Desmoglein 1/DSG1 antibody [EPR20382] ab209490).
Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling Desmoglein 1/DSG1 with Anti-Desmoglein 1/DSG1 antibody [EPR20382] ab209490 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) ready to use. Membranous staining on human esophagus (PMID: 24220297) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat-mediated antigen retrieval using Universal HIER antigen retrieval reagent (10X) ab208572 (Universal HIER antigen retrieval reagent).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Desmoglein 1/DSG1 antibody [EPR20382] ab209490).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Desmoglein 1/DSG1 antibody [EPR20382] ab209490).
Panel A: merged staining of anti-Desmoglein 1/DSG1 (gray; Opal™690), anti-Langerin (green; Opal™520) and anti-TRP2/DCT (red; Opal™570) on human skin.
Panel B: anti-TRP2/DCT stained on melanocytes.
Panel C: anti-Langerin stained on Langerhans cells.
Panel D: anti-Desmoglein 1/DSG1 stained on epidermis.
The section was incubated in three rounds of staining: in the order of ab236259, Anti-Langerin antibody [EPR24685-12] ab283686, and Anti-TRP2/DCT antibody [EPR21986] ab221144 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
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