AB124798

Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

Name: Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) | Abcam
Brand: Abcam Limited
SKU: AB124798
Price: 380 USD
Availability: InStock
Rating: 5 (5 reviews)

RabMAb
Recombinant
What is this?

(5 Reviews)

(18 Publications)

Rabbit Recombinant Monoclonal Desmoglein 1/DSG1 antibody. Suitable for WB and reacts with Human samples. Cited in 18 publications.

View Alternative Names

CDHF4, DSG1, Desmoglein-1, Cadherin family member 4, Desmosomal glycoprotein 1, Pemphigus foliaceus antigen, DG1, DGI

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

ab124798, at a 1/250 dilution, staining Desmoglein 1/DSG1 in paraffin embedded Human skin tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as GAPDH loading control. We recommend using 1%SDS Hot lysis prepare method to get desired Western Blot results.

All lanes:

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/10000 dilution

Lane 1:

Human skin lysate prepared in RIPA lysis method at 20 µg

Lane 2:

Human skin lysate prepared in 1%SDS Hot lysis method at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/20000 dilution

Predicted band size: 114 kDa

Observed band size: 160 kDa

false

Exposure time: 20s

Unknown

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

All lanes:

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (ab124798) at 1/1000 dilution

All lanes:

Human skin lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 114 kDa

false

Carrier free

Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6766(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "antigen retrieval is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 1 often abbreviated as DSG1 or Desmoglein-1 is a protein that plays a significant mechanical role in cell adhesion. This protein with a molecular mass of approximately 122 kDa belongs to the cadherin superfamily. It is mainly expressed in the epidermis specifically in the outermost layers of the skin. Desmoglein 1 is an important component of desmosomes which are specialized cell structures responsible for maintaining the integrity of epithelial tissues.

Biological function summary

Desmogleins including Desmoglein 1 facilitate adhesion between keratinocytes the predominant cell type in the epidermis. Desmoglein 1 interacts intimately within the desmosome complex contributing to the mechanical stability and cohesion of the cellular layers in the skin. These interactions help form a barrier that protects underlying tissues from environmental assaults and water loss. It is important in the process of epidermal differentiation important for proper skin formation structure and function.

Pathways

Desmoglein 1 integrates into several signaling and structural pathways related to cell adhesion and epidermal maintenance. Desmoglein 1 is prominently involved in the Wnt signaling pathway which is important for skin development and renewal. This involvement establishes its functional link with other adhesion proteins such as desmocollins and regulatory molecules like plakoglobin which mediate the cytoskeletal association within desmosomes.

Desmoglein 1 has clinical relevance in conditions such as pemphigus foliaceus and skin inflammation disorders. Antibodies targeting DSG1 often referred to as anti-Desmoglein 1 antibodies play a pathogenic role in pemphigus foliaceus disrupting cell adhesion and resulting in blistering. Additionally Desmoglein 1's interaction with other proteins like Desmoglein 3 becomes altered in certain auto-immune diseases disturbing normal skin integrity and leading to disease phenotypes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Component of intercellular desmosome junctions (PubMed : 34368962). Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion (PubMed : 19717567).

See full target information DSG1

style

left-column

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 34:e70145 PubMed40734331

2025

Mechanism Study on Inhibition of EPHA2 Expression Impaired Skin Barrier Function by Gefitinib.

Applications

Unspecified application

Species

Unspecified reactive species

Jiang-Yuan Zhang,Xue-Kun Nie,Zi-Chun Chen,Su-Juan Zhou,Xiao-Hui Lin,Li Zhang,Di Zhong,Bing-Ying Xiao,Shi-Qing Jiang,Wei-Ying Huang,Min-Hua Lin,Yu-Jia Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403627 PubMed39535354

2024

Spatiotemporal Molecular Architecture of Lineage Allocation and Cellular Organization in Tooth Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shengjie Jiang,Yuning Zhang,Huimin Zheng,Kai Zhao,Yue Yang,Binbin Lai,Xuliang Deng,Yan Wei

Nature immunology 25:2068-2084 PubMed39354200

2024

MrgprA3 neurons drive cutaneous immunity against helminths through selective control of myeloid-derived IL-33.

Applications

Unspecified application

Species

Unspecified reactive species

Juan M Inclan-Rico,Camila M Napuri,Cailu Lin,Li-Yin Hung,Annabel A Ferguson,Xiaohong Liu,Qinxue Wu,Christopher F Pastore,Adriana Stephenson,Ulrich M Femoe,Fungai Musaigwa,Heather L Rossi,Bruce D Freedman,Danielle R Reed,Tomáš Macháček,Petr Horák,Ishmail Abdus-Saboor,Wenqin Luo,De'Broski R Herbert

Developmental cell 57:2683-2698.e8 PubMed36495876

2022

Epidermal stratification requires retromer-mediated desmoglein-1 recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Marihan Hegazy,Jennifer L Koetsier,Amber L Huffine,Joshua A Broussard,Brendan M Godsel,Eran Cohen-Barak,Eli Sprecher,Donald J Wolfgeher,Stephen J Kron,Lisa M Godsel,Kathleen J Green

Experimental dermatology 31:214-222 PubMed34379845

2021

Acral peeling in Nagashima type palmo-plantar keratosis patients reveals the role of serine protease inhibitor B 7 in keratinocyte adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Eran Cohen-Barak,Wassim Azzam,Jennifer L Koetsier,Nada Danial-Farran,Moran Barcan,Maysa Hriesh,Morad Khayat,Natalia Edison,Judith Krausz,Chen Gafni-Amsalem,Akiharu Kubo,Lisa M Godsel,Michael Ziv,Stavit Allon-Shalev

BMC pulmonary medicine 21:252 PubMed34332565

2021

Emodin inhibits viability, proliferation and promotes apoptosis of hypoxic human pulmonary artery smooth muscle cells via targeting miR-244-5p/DEGS1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yi,JunFang Liu,Ming Deng,Huihua Zuo,Mingyan Li

Cell host & microbe 29:1235-1248.e8 PubMed34214492

2021

Commensal microbiota regulates skin barrier function and repair via signaling through the aryl hydrocarbon receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Aayushi Uberoi,Casey Bartow-McKenney,Qi Zheng,Laurice Flowers,Amy Campbell,Simon A B Knight,Neal Chan,Monica Wei,Victoria Lovins,Julia Bugayev,Joseph Horwinski,Charles Bradley,Jason Meyer,Debra Crumrine,Carrie Hayes Sutter,Peter Elias,Elizabeth Mauldin,Thomas R Sutter,Elizabeth A Grice

Journal of cosmetic dermatology 20:1002-1008 PubMed32757243

2020

Biophysical characteristics of dandruff-affected scalp categorized on the basis of sebum levels.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Seon Yoon,Jiyong Shim,Jun-Man Lim,Sun Gyoo Park

The Laryngoscope 131:839-845 PubMed32658340

2020

Restricted Water Intake Adversely Affects Rat Vocal Fold Biology.

Applications

Unspecified application

Species

Unspecified reactive species

Chenwei Duan,Naila Cannes do Nascimento,Sarah Calve,Abigail Cox,M Preeti Sivasankar

Developmental cell 52:764-778.e4 PubMed32109382

2020

Epithelial Migration and Non-adhesive Periderm Are Required for Digit Separation during Mammalian Development.

Applications

Unspecified application

Species

Unspecified reactive species

Ghaidaa Kashgari,Lina Meinecke,William Gordon,Bryan Ruiz,Jady Yang,Amy Lan Ma,Yilu Xie,Hsiang Ho,Maksim V Plikus,Qing Nie,James V Jester,Bogi Andersen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

Western blot - Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)] (AB124798)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise