AB96761

Anti-Desmoglein 2/DSG2 antibody

Name: Anti-Desmoglein 2/DSG2 antibody. Rabbit polyclonal (ab96761) | Abcam
Brand: Abcam Limited
SKU: AB96761
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Desmoglein 2/DSG2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DSG2 aa 1-200.

View Alternative Names

CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody (AB96761)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Immunohistochemical analysis of Desmoglein 2/DSG2 in paraffin embedded DU145 (Human) xenograft, using ab96761 at a 1/100 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody (AB96761)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution (green) in ICC/IF.

The nuclear counter stain is Hoechst 33342 (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Paraffin-embedded mouse skin labeling Desmoglein 2/DSG2 using ab96761 at 1/500 dilution in immunohistochemical analysis.

Antigen retrieval : EDTA-based (pH 8.0) 15 minutes.

Unknown

Western blot - Anti-Desmoglein 2/DSG2 antibody (AB96761)

7.5% SDS Page

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

H1299 whole cell lysate at 30 µg

Lane 3:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 122 kDa

false

Supplier Data

Western blot - Anti-Desmoglein 2/DSG2 antibody (AB96761)

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody (ab96761) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 30 µg

Secondary

All lanes:

Anti-Rabbit IgG HRP

Predicted band size: 122 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DSG2 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q14126

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.

Biological function summary

DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.

Pathways

DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.

DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

See full target information DSG2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 18:30405-30420 PubMed39453865

2024

Mitochondrial Bioenergetics of Functional Wound Closure is Dependent on Macrophage-Keratinocyte Exosomal Crosstalk.

Applications

Unspecified application

Species

Unspecified reactive species

Anu Sharma,Rajneesh Srivastava,Surya C Gnyawali,Pramod Bhasme,Adam J Anthony,Yi Xuan,Jonathan C Trinidad,Chandan K Sen,David E Clemmer,Sashwati Roy,Subhadip Ghatak

Stem cells international 2023:2826815 PubMed37964983

2023

A Novel Molecular Classification Method for Glioblastoma Based on Tumor Cell Differentiation Trajectories.

Applications

Unspecified application

Species

Unspecified reactive species

Guanghao Zhang,Xiaolong Xu,Luojiang Zhu,Sisi Li,Rundong Chen,Nan Lv,Zifu Li,Jing Wang,Qiang Li,Wang Zhou,Pengfei Yang,Jianmin Liu

Virologica Sinica 36:1400-1410 PubMed34224109

2021

Desmoglein 2 (DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Kui Ma,Xiangyu Wang,Yinbo Jiang,Shan Zhao,Junxian Ou,Wendong Lan,Wenyi Guan,Xiaowei Wu,Heping Zheng,Bin Yang,Chengsong Wan,Wei Zhao,Jianguo Wu,Qiwei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 2/DSG2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Western blot - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Western blot - Anti-Desmoglein 2/DSG2 antibody (AB96761)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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