Rabbit Polyclonal Desmoglein 2/DSG2 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DSG2 aa 1050 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human DSG2 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14126
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chimpanzee, Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Chimpanzee, Cynomolgus monkey, Rhesus monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.
Alternative names
CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC
Recommended products
Rabbit Polyclonal Desmoglein 2/DSG2 antibody. C-terminal. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human DSG2 aa 1050 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human DSG2 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q14126
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab226258 was affinity purified using an epitope specific to Desmoglein 2/DSG2 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.
- Biological function summary
DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.
- Pathways
DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.
- Associated diseases and disorders
DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Desmoglein 2/DSG2 antibody - C-terminal (ab226258)
All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody - C-terminal (ab226258) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 122 kDa
Exposure time: 3s
Immunoprecipitation - Anti-Desmoglein 2/DSG2 antibody - C-terminal (ab226258)
Desmoglein 2/DSG2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226258 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab226258 at 1 μg/ml.
Lane 1: ab226258 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 1 second.
All lanes: Immunoprecipitation - Anti-Desmoglein 2/DSG2 antibody - C-terminal (ab226258)
Predicted band size: 122 kDa
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