Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] KO Tested (ab315448)

Knockout Tested Rabbit Recombinant Monoclonal Desmoglein 2/DSG2 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (AB315448), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	IHC-P	Flow Cyt	IP
Human	Tested	Tested	Tested	Not recommended	Not recommended
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/8000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information DSG2

Function

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Alternative names

CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Desmoglein 2/DSG2 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26639-18
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.

Biological function summary

DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.

Pathways

DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.

Associated diseases and disorders

DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Low expression: Daudi(PMID: 29301346)
The identity of the band between 50 kDa and 37 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448) at 1/1000 dilution
Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: Daudi (human Burkitt’s lymphoma lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 90-160 kDa, 36 kDa
Exposure time: 3m
Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Low expression: skeletal muscle (PMID: 22457526).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 90-160 kDa, 36 kDa
Exposure time: 37s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Desmoglein 2/DSG2 with ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on human liver. The section was incubated with ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Desmoglein 2/DSG2 with ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human ovarian carcinoma. The section was incubated with ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Desmoglein 2/DSG2 with ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human lung carcinoma (PMID: 33217893). The section was incubated with ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Desmoglein 2/DSG2 with ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human endometrium. The section was incubated with ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
In Western blot, ab315448 was shown to bind specifically to Desmoglein 2/DSG2. A band was observed at 90-160 kDa in wild-type HeLa cell lysates with whereas no signal observed at this size in Desmoglein 2/DSG2 knockout cell line Human DSG2 (Desmoglein 2) knockout HeLa cell line ab261826 (knockout cell lysate Human DSG2 (Desmoglein 2) knockout HeLa cell lysate ab257158).
The expression molecular weight observed is consistent with what has been described in the literature (PMID: 21841821).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 2: DSG2 knockout HeLa whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 90-160 kDa, 36 kDa
Exposure time: 37s
Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Low expression: liver.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448) at 1/1000 dilution
Lane 1: Human colon tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Human liver tissue lysate at 20 µg with 5% NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 90-160 kDa, 36 kDa
Exposure time: 15s
Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (ab315448)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized DSG2 KO HeLa (DSG2 knockout human cervical adenocarcinoma epithelial cell), Human DSG2 (Desmoglein 2) knockout HeLa cell line ab261826 cells labelling Desmoglein 2/DSG2 with ab315448 at 1/1000 (0.517 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing mainly membranous staining in wildtype HeLa cells, showing no staining in DSG2 knockout HeLa cells.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.