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Knockout Tested Rabbit Recombinant Monoclonal Desmoglein 2/DSG2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (AB315449), expandable thumbnail
  • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (AB315449), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (AB315449), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (AB315449), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (AB315449), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PFlow CytIP
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Alternative names

CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal Desmoglein 2/DSG2 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26639-18
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315449 is the carrier-free version of Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.

Biological function summary

DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.

Pathways

DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.

Associated diseases and disorders

DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Low expression: Daudi(PMID: 29301346)

    The identity of the band between 50 kDa and 37 kDa is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Daudi (human Burkitt’s lymphoma lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 90-160 kDa, 36 kDa

    Exposure time: 3m

  • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Low expression: skeletal muscle (PMID: 22457526).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human skeletal muscle tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 90-160 kDa, 36 kDa

    Exposure time: 37s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Desmoglein 2/DSG2 with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: no staining on human liver. The section was incubated with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Desmoglein 2/DSG2 with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human ovarian carcinoma. The section was incubated with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling Desmoglein 2/DSG2 with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human lung carcinoma (PMID: 33217893). The section was incubated with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling Desmoglein 2/DSG2 with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 at 1/8000 (0.065 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on human endometrium. The section was incubated with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 was shown to bind specifically to Desmoglein 2/DSG2. A band was observed at 90-160 kDa in wild-type HeLa cell lysates with whereas no signal observed at this size in Desmoglein 2/DSG2 knockout cell line Human DSG2 (Desmoglein 2) knockout HeLa cell line ab261826 (knockout cell lysate Human DSG2 (Desmoglein 2) knockout HeLa cell lysate ab257158).

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 21841821).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: DSG2 knockout HeLa whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 90-160 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Low expression: liver.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] (Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Human liver tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 90-160 kDa, 36 kDa

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] - BSA and Azide free (ab315449)

    This data was developed using Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized DSG2 KO HeLa (DSG2 knockout human cervical adenocarcinoma epithelial cell), Human DSG2 (Desmoglein 2) knockout HeLa cell line ab261826 cells labelling Desmoglein 2/DSG2 with Anti-Desmoglein 2/DSG2 antibody [EPR26639-18] ab315448 at 1/1000 (0.517 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

    Confocal image showing mainly membranous staining in wildtype HeLa cells, showing no staining in DSG2 knockout HeLa cells.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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