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AB150372

Anti-Desmoglein 2/DSG2 antibody [EPR6768]

  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
  • What is this?

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(2 Reviews)

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(26 Publications)

Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) is a rabbit monoclonal antibody detecting Desmoglein 2/DSG2 in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- KO validated for confirmed specificity
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

View Alternative Names

CDHF5, DSG2, Desmoglein-2, Cadherin family member 5, HDGC

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)

Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)
  • WB

Lab

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)

Lanes 1- 2 : Merged signal (red and green). Green - ab150372 observed at 90-160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab150372 was shown to react with Desmoglein 2/DSG2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261826 (knockout cell lysate ab257158) was used. Wild-type HeLa and DSG2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150372 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

DSG2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human DSG2 (Desmoglein 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-dsg2-desmoglein-2-knockout-hela-cell-line-ab261826'>ab261826</a>)

Predicted band size: 122 kDa

Observed band size: 90-160 kDa

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Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)
  • WB

Lab

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)

Lanes 1 - 6 : Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution

Lane 1:

Trypsinised HAP1 cell lysate at 40 µg

Lane 2:

Scraped HAP1 cell lysate at 40 µg

Lane 3:

Trypsinised DSG2 knockout HAP1 cell lysate at 40 µg

Lane 4:

Scraped DSG2 knockout HAP1 cell lysate at 40 µg

Lane 5:

Trypsinised A431 cell lysate at 40 µg

Lane 6:

Scraped A431 cell lysate at 40 µg

Predicted band size: 122 kDa

Observed band size: 95 kDa

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Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)
  • WB

Unknown

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (AB150372)

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

mouse heart lysate at 10 µg

Lane 5:

rat heart lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 122 kDa

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  • Carrier free

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - BSA and Azide free

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Desmoglein 2/DSG2 antibody [EPR6768]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Desmoglein 2/DSG2 antibody [EPR6768]

  • Carrier free

    Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6768

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

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Complementary Products

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Secondary Antibodies

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Primary Antibodies

AB124798

Anti-Desmoglein 1/DSG1 antibody [EPR6766(B)]

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of Desmoglein 2/DSG2?
Anti-Desmoglein 2/DSG2 [EPR6768] (ab150372) specifically detects a band for Desmoglein 2/DSG2 (UniProt: Q14126) at a molecular weight of 122kDa.

Trusted by the scientific community
Anti-Desmoglein 2/DSG2 [EPR6768] (ab150372) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed
The specificity of Anti-Desmoglein 2/DSG2 antibody [EPR6768] (ab150372) has been confirmed by Western blot testing in DSG2 Knockout HAP1 cells.

Other related products
We have a range of other formats of antibody clone [EPR6768] also available for your convenience: ab150372, Carrier free - ab230833, Carrier free - ab271913, Alexa Fluor® 647 - ab322025, Alexa Fluor® 488 - ab322421

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 2 (DSG2) is a member of the desmoglein family of proteins sometimes referred to as desmoglein-2 or DSG 2. This protein is a calcium-binding transmembrane glycoprotein and part of the cadherin family with a molecular mass of approximately 122 kDa. DSG2 plays a mechanical role in cell-cell adhesion by forming desmosomes which are specialized structures for maintaining the integrity of tissues. DSG2 is mainly expressed in epithelial cells including those found in the heart skin and certain internal organs.
Biological function summary

DSG2 contributes to the structural stability of tissues by locking cells together forming a mechanical and signaling function. The protein is integral to the composition of desmosomes complexes that include other components such as plakoglobin and desmoplakin. These complexes strengthen intercellular adhesion which is essential for tissues that experience constant physical stress like the heart and skin. DSG2 actively participates in cell signaling impacting cellular behavior and communication.

Pathways

DSG2 plays significant roles in the heart development and epidermal differentiation pathways. DSG2 interacts with several other proteins including plakoglobin and desmoplakin to maintain cellular adhesion and tissue morphogenesis. In the cardiac setting the heart development pathway connects DSG2 with other proteins essential for maintaining the integrity and signaling within the cardiac muscle. This interaction helps modulate pathways that ensure proper cardiac function and repair processes.

DSG2 is linked to arrhythmogenic right ventricular cardiomyopathy (ARVC) which affects the heart's rhythm and muscle structure. DSG2 mutations can disrupt desmosomal structure weakening cell adhesion and leading to disease. Another related disorder is pemphigus vulgaris an autoimmune disease where antibodies target DSG2 among other desmogleins disrupting cell adhesion in the skin and mucous membranes. In ARVC DSG2 frequently connects with other proteins like plakoglobin that are involved in desmosomal interactions leading to the structural and functional deficits seen in the disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 17559062, PubMed : 38395410). Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Required for proliferation and viability of embryonic stem cells in the blastocyst, thereby crucial for progression of post-implantation embryonic development (By similarity). Maintains pluripotency by regulating epithelial to mesenchymal transition/mesenchymal to epithelial transition (EMT/MET) via interacting with and sequestering CTNNB1 to sites of cell-cell contact, thereby reducing translocation of CTNNB1 to the nucleus and subsequent transcription of CTNNB1/TCF-target genes (PubMed : 29910125). Promotes pluripotency and the multi-lineage differentiation potential of hematopoietic stem cells (PubMed : 27338829). Plays a role in endothelial cell sprouting and elongation via mediating the junctional-association of cortical actin fibers and CDH5 (PubMed : 27338829). Promotes cardiomyocyte cell homeostasis and desmosome junction formation at intercalated disks, as a result plays a role in the maintenance of cardiac conduction and heart chamber integrity (By similarity). Positively regulates pancreatic islet development and maintenance of endothelial cell barrier integrity in the pancreas, therefore involved in the controlled release of insulin from islet cells into the circulation in response to glucose (By similarity). Plays a role in limiting inflammatory infiltration and the apoptotic response to injury in kidney tubular epithelial cells, potentially via its role in maintaining cell-cell adhesion and the epithelial barrier (PubMed : 38395410). Acts as a positive modulator of CSK and EGFR activation via sequestering them away from lipid rafts, this is independent of its role in desmosome cell junctions (PubMed : 26918609). Also disrupts the localization of CAV1 to lipid rafts resulting in its distribution throughout the cytoplasm (PubMed : 26918609).
See full target information DSG2
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology 27: PubMed40879390

2025

Comprehensive analysis of desmosomal protein remodelling identifies desmocollin-2 as potential biomarker for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Zhenliang Hu,Lingmin Wu,Deniz Akdis,Weiquan Ye,Ardan M Saguner,Mingming Su,Hongbin Dong,Zhongli Chen,Dan Hu,Shengshou Hu,Firat Duru,Liang Chen

International journal of molecular sciences 24: PubMed37446408

2023

EPs 7630 Stimulates Tissue Repair Mechanisms and Modifies Tight Junction Protein Expression in Human Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fang,Liang Zhou,Žarko Kulić,Martin D Lehner,Michael Tamm,Michael Roth

Scientific reports 13:10586 PubMed37391503

2023

Telomere-related prognostic biomarkers for survival assessments in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shengyang Chen,Shuiquan Hu,Baizhong Zhou,Bingbing Cheng,Hao Tong,Dongchao Su,Xiaoyong Li,Yanjun Chen,Genhao Zhang

Molecular cancer research : MCR 21:836-848 PubMed37115197

2023

Desmoglein 2 Functions as a Receptor for Fatty Acid Binding Protein 4 in Breast Cancer Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Chen,Keith M Wirth,Scott Kizy,Joseph M Muretta,Todd W Markowski,Peter Yong,Adam Sheka,Hisham Abdelwahab,Ann V Hertzel,Sayeed Ikramuddin,Masato Yamamoto,David A Bernlohr

Computational and structural biotechnology journal 21:1759-1773 PubMed36915380

2023

Modeling incomplete penetrance in arrhythmogenic cardiomyopathy by human induced pluripotent stem cell derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Marzia De Bortoli,Viviana Meraviglia,Katarina Mackova,Laura S Frommelt,Eva König,Johannes Rainer,Chiara Volani,Patrizia Benzoni,Maja Schlittler,Giada Cattelan,Benedetta M Motta,Claudia Volpato,Werner Rauhe,Andrea Barbuti,Serena Zacchigna,Peter P Pramstaller,Alessandra Rossini

International journal of molecular sciences 24: PubMed36768439

2023

Alterations in Calcium Handling Are a Common Feature in an Arrhythmogenic Cardiomyopathy Cell Model Triggered by Desmosome Genes Loss.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vallverdú-Prats,David Carreras,Guillermo J Pérez,Oscar Campuzano,Ramon Brugada,Mireia Alcalde

Tissue barriers 11:2138061 PubMed36280901

2022

Plakophilin 2 regulates intestinal barrier function by modulating protein kinase C activity in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Nagler,Yalda Ghoreishi,Catherine Kollmann,Matthias Kelm,Brenda Gerull,Jens Waschke,Natalie Burkard,Nicolas Schlegel

Nature communications 13:6166 PubMed36257954

2022

Tmem65 is critical for the structure and function of the intercalated discs in mouse hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Allen C T Teng,Liyang Gu,Michelle Di Paola,Robert Lakin,Zachary J Williams,Aaron Au,Wenliang Chen,Neal I Callaghan,Farigol Hakem Zadeh,Yu-Qing Zhou,Meena Fatah,Diptendu Chatterjee,L Jane Jourdan,Jack Liu,Craig A Simmons,Thomas Kislinger,Christopher M Yip,Peter H Backx,Robert G Gourdie,Robert M Hamilton,Anthony O Gramolini

International journal of molecular sciences 23: PubMed35054841

2022

Premature Termination Codon in 5' Region of Desmoplakin and Plakoglobin Genes May Escape Nonsense-Mediated Decay through the Reinitiation of Translation.

Applications

Unspecified application

Species

Unspecified reactive species

Marta Vallverdú-Prats,Ramon Brugada,Mireia Alcalde

Cell and tissue research 386:79-98 PubMed34236518

2021

Early inflammation precedes cardiac fibrosis and heart failure in desmoglein 2 murine model of arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

K E Ng,P J Delaney,D Thenet,S Murtough,C M Webb,N Zaman,E Tsisanova,G Mastroianni,S L M Walker,J D Westaby,D J Pennington,R Pink,D P Kelsell,A Tinker
View all publications
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