Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free (AB230833)

This IHC data was generated using the same anti-Desmoglein 2/DSG2 antibody clone EPR6768 in a different buffer formulation (cat# ab150372).

Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Desmoglein 2/DSG2 with ab150372 at a 1/250 dilution.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free (AB230833)

This data was developed using the same antibody clone in a different buffer formulation (ab150372).

Lanes 1- 2 : Merged signal (red and green). Green - ab150372 observed at 90-160 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab150372 was shown to react with Desmoglein 2/DSG2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261826 (knockout cell lysate ab257158) was used. Wild-type HeLa and DSG2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab150372 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (<a href='/en-us/products/primary-antibodies/desmoglein-2-dsg2-antibody-epr6768-ab150372'>ab150372</a>) at 1/10000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

DSG2 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human DSG2 (Desmoglein 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-dsg2-desmoglein-2-knockout-hela-cell-line-ab261826'>ab261826</a>)

Predicted band size: 122 kDa

Observed band size: 90-160 kDa

false

• WB

Lab

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] - Low endotoxin, Azide free (AB230833)

This data was developed using the same antibody clone in a different buffer formulation (ab150372).

Lanes 1 - 6 : Merged signal (red and green). Green - ab150372 observed at 95 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab150372 was shown to react with Desmoglein 2 in wild-type HAP1 cells in western blot with loss of signal observed in DSG2 knockout sample. Wild-type and DSG2 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab150372 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Desmoglein 2/DSG2 antibody [EPR6768] (<a href='/en-us/products/primary-antibodies/desmoglein-2-dsg2-antibody-epr6768-ab150372'>ab150372</a>) at 1/10000 dilution

Lane 1:

Trypsinised HAP1 cell lysate at 40 µg

Lane 2:

Scraped HAP1 cell lysate at 40 µg

Lane 3:

Trypsinised DSG2 knockout HAP1 cell lysate at 40 µg

Lane 4:

Scraped DSG2 knockout HAP1 cell lysate at 40 µg

Lane 5:

Trypsinised A431 cell lysate at 40 µg

Lane 6:

Scraped A431 cell lysate at 40 µg

Predicted band size: 122 kDa

Observed band size: 95 kDa

false