AB14416

Anti-Desmoglein 3/PVA antibody [5G11]

Name: Anti-Desmoglein 3/PVA antibody [5G11] (ab14416) | Abcam
Brand: Abcam Limited
SKU: AB14416
Price: 495 USD
Availability: InStock
Rating: 5 (2 reviews)

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What is this?

(2 Reviews)

(5 Publications)

Mouse Monoclonal Desmoglein 3/PVA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CDHF6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, PVA

2 Images

IHC-P

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416)

IHC image of Desmoglein 3/PVA staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14416, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

AbReview33177****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416)

Immunohistochemical analysis of Human tongue tissue, staining Desmoglein 3/PVA with ab14416.

Tissue was fixed with formaldehyde and antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/500 in Tris buffered saline with 0.1% Tween 20) for 18 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/2) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5G11

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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Product details

The clone number has been updated from 3G133 to 5G11 since both name the same clone and 5G11 is the original clone number.

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Add glycerol to a final volume of 50% for extra stability and aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Desmoglein 3 (DSG3) also known as anti-DSG3 is an important component in maintaining cellular adhesion within epithelial tissues. It belongs to the cadherin family and has a mass of approximately 130 kDa. You will find DSG3 expressed mainly in epithelial cells particularly in the skin and mucosal linings. As a transmembrane glycoprotein DSG3 interacts with other desmoglein proteins such as desmoglein 1 to form desmosomes which are essential for the mechanical coupling of cells.

Biological function summary

This protein plays a fundamental role in cell adhesion by contributing to the integrity and strength of desmosomes. These are multi-protein complexes that secure cell-to-cell attachments in tissues exposed to mechanical stress. Within the desmosomes DSG3 interacts closely with other proteins including plakoglobin and plakophilins ensuring the stability of the cellular junctions. This makes DSG3 an integral part of maintaining tissue structure and resilience.

Pathways

Desmoglein 3 participates notably in the WNT signaling pathway and is involved in pathways associated with cell adhesion. In these pathways its interaction with plakoglobin which is also known as gamma-catenin plays an important role. This interaction influences signaling processes that modulate cellular adhesion and proliferation. Desmosomes facilitated by DSG3 are connective points in epidermal growth regulation pathways emphasizing DSG3's involvement in cellular cohesion and communication.

Desmoglein 3 is strongly linked to the autoimmune disease pemphigus vulgaris. In this condition autoantibodies target DSG3 disrupting its function in desmosomes and leading to blistering of the skin and mucous membranes. Desmoglein 3's relationship with desmoglein 1 is also relevant in the disease process as these proteins' shared function in cell adhesion becomes compromised. Therefore understanding DSG3's interactions and functions is vital for exploring therapeutic strategies in treating such disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 31835537). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (PubMed : 31835537). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed : 9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (PubMed : 22294297). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (PubMed : 31835537). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (PubMed : 31835537). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (PubMed : 22796473). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (PubMed : 22294297). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed : 26763450).

See full target information DSG3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 13:886226 PubMed35911677

2022

Paraneoplastic Pemphigus Autoantibodies Against C-terminus of Desmoplakin Induced Acantholysis and .

Applications

Unspecified application

Species

Unspecified reactive species

Xue Wang,Rui Wang,Dingfang Bu,Leyi Wang,Yuexin Zhang,Yuan Chang,Chenyang Zhang,Xixue Chen,Xuejun Zhu,Zhi Liu,Mingyue Wang

Health science reports 5:e449 PubMed35028432

2022

Optimizing large-scale autologous human keratinocyte sheets for major burns-Toward an animal-free production and a more accessible clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Frese,Salim Elias Darwiche,Myrna Elisabeth Gunning,Simon Philipp Hoerstrup,Brigitte von Rechenberg,Pietro Giovanoli,Maurizio Calcagni

Cytotherapy 23:536-547 PubMed33685808

2021

Thermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Frese,Salim E Darwiche,Brigitte von Rechenberg,Simon P Hoerstrup,Pietro Giovanoli,Maurizio Calcagni

American journal of cancer research 11:495-512 PubMed33575083

2021

A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.

Applications

Unspecified application

Species

Unspecified reactive species

Lisi Luo,Yufang Li,Chumei Huang,Yujing Lin,Yonghui Su,Hong Cen,Yutong Chen,Siqi Peng,Tianyi Ren,Rongzhi Xie,Linjuan Zeng

Contemporary oncology (Poznan, Poland) 20:374-380 PubMed28373818

2016

Determining the potential of desmoglein 3 as a sensitive and specific immunohistochemical marker for the detection of micrometastasis in patients with primary oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shruti Nagvekar,Anita Spadigam,Anita Dhupar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmoglein 3/PVA antibody [5G11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise