Mouse Monoclonal Desmoglein 3/PVA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-3-pva-antibody-5g11-ab14416--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46679.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-3-pva-antibody-5g11-ab14416--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132258.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (AB14416)")
Publications
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- Frontiers in immunology 13:8862262022Paraneoplastic Pemphigus Autoantibodies Against C-terminus of Desmoplakin Induced Acantholysis and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Wang,Rui Wang,Dingfang Bu,Leyi Wang,Yuexin Zhang,Yuan Chang,Chenyang Zhang,Xixue Chen,Xuejun Zhu,Zhi Liu,Mingyue WangPubMed 35911677
- Health science reports 5:e4492022Optimizing large-scale autologous human keratinocyte sheets for major burns-Toward an animal-free production and a more accessible clinical application.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Frese,Salim Elias Darwiche,Myrna Elisabeth Gunning,Simon Philipp Hoerstrup,Brigitte von Rechenberg,Pietro Giovanoli,Maurizio CalcagniPubMed 35028432
- Cytotherapy 23:536-5472021Thermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Frese,Salim E Darwiche,Brigitte von Rechenberg,Simon P Hoerstrup,Pietro Giovanoli,Maurizio CalcagniPubMed 33685808
- American journal of cancer research 11:495-5122021A new 7-gene survival score assay for pancreatic cancer patient prognosis prediction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisi Luo,Yufang Li,Chumei Huang,Yujing Lin,Yonghui Su,Hong Cen,Yutong Chen,Siqi Peng,Tianyi Ren,Rongzhi Xie,Linjuan ZengPubMed 33575083
- Contemporary oncology (Poznan, Poland) 20:374-3802016Determining the potential of desmoglein 3 as a sensitive and specific immunohistochemical marker for the detection of micrometastasis in patients with primary oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShruti Nagvekar,Anita Spadigam,Anita DhuparPubMed 28373818
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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Target data
Function
A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed:31835537). Required for adherens and desmosome junction assembly in response to mechanical force in keratinocytes (PubMed:31835537). Required for desmosome-mediated cell-cell adhesion of cells surrounding the telogen hair club and the basal layer of the outer root sheath epithelium, consequently is essential for the anchoring of telogen hairs in the hair follicle (PubMed:9701552). Required for the maintenance of the epithelial barrier via promoting desmosome-mediated intercellular attachment of suprabasal epithelium to basal cells (By similarity). May play a role in the protein stability of the desmosome plaque components DSP, JUP, PKP1, PKP2 and PKP3 (PubMed:22294297). Required for YAP1 localization at the plasma membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, PKP1 and YWHAG (PubMed:31835537). May also be involved in the positive regulation of YAP1 target gene transcription and as a result cell proliferation (PubMed:31835537). Positively regulates cellular contractility and cell junction formation via organization of cortical F-actin bundles and anchoring of actin to tight junctions, in conjunction with RAC1 (PubMed:22796473). The cytoplasmic pool of DSG3 is required for the localization of CDH1 and CTNNB1 at developing adherens junctions, potentially via modulation of SRC activity (PubMed:22294297). Inhibits keratinocyte migration via suppression of p38MAPK signaling, may therefore play a role in moderating wound healing (PubMed:26763450).
Alternative names
CDHF6, DSG3, Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, PVA
Recommended products
Mouse Monoclonal Desmoglein 3/PVA antibody. Suitable for IHC-P and reacts with Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 5G11
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Add glycerol to a final volume of 50% for extra stability and aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The clone number has been updated from 3G133 to 5G11 since both name the same clone and 5G11 is the original clone number.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Desmoglein 3 (DSG3) also known as anti-DSG3 is an important component in maintaining cellular adhesion within epithelial tissues. It belongs to the cadherin family and has a mass of approximately 130 kDa. You will find DSG3 expressed mainly in epithelial cells particularly in the skin and mucosal linings. As a transmembrane glycoprotein DSG3 interacts with other desmoglein proteins such as desmoglein 1 to form desmosomes which are essential for the mechanical coupling of cells.
- Biological function summary
This protein plays a fundamental role in cell adhesion by contributing to the integrity and strength of desmosomes. These are multi-protein complexes that secure cell-to-cell attachments in tissues exposed to mechanical stress. Within the desmosomes DSG3 interacts closely with other proteins including plakoglobin and plakophilins ensuring the stability of the cellular junctions. This makes DSG3 an integral part of maintaining tissue structure and resilience.
- Pathways
Desmoglein 3 participates notably in the WNT signaling pathway and is involved in pathways associated with cell adhesion. In these pathways its interaction with plakoglobin which is also known as gamma-catenin plays an important role. This interaction influences signaling processes that modulate cellular adhesion and proliferation. Desmosomes facilitated by DSG3 are connective points in epidermal growth regulation pathways emphasizing DSG3's involvement in cellular cohesion and communication.
- Associated diseases and disorders
Desmoglein 3 is strongly linked to the autoimmune disease pemphigus vulgaris. In this condition autoantibodies target DSG3 disrupting its function in desmosomes and leading to blistering of the skin and mucous membranes. Desmoglein 3's relationship with desmoglein 1 is also relevant in the disease process as these proteins' shared function in cell adhesion becomes compromised. Therefore understanding DSG3's interactions and functions is vital for exploring therapeutic strategies in treating such disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-3-pva-antibody-5g11-ab14416--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img46679.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416)
IHC image of Desmoglein 3/PVA staining in Human skin melanoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14416, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416), expandable thumbnail](https://content.abcam.com/products/images/desmoglein-3-pva-antibody-5g11-ab14416--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img132258.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416)")
This image is courtesy of an anonymous customer reviewImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmoglein 3/PVA antibody [5G11] (ab14416)
Immunohistochemical analysis of Human tongue tissue, staining Desmoglein 3/PVA with ab14416.
Tissue was fixed with formaldehyde and antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/500 in Tris buffered saline with 0.1% Tween 20) for 18 hours at 4°C. An HRP-conjugated goat anti-mouse polyclonal IgG (1/2) was used as the secondary antibody.
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